Adaptive optics two photon microscopy with direct wavefront sensing using autofluorescent guide-stars

2014 ◽  
Author(s):  
Xiaodong Tao ◽  
Andrew Norton ◽  
Matthew Kissel ◽  
Oscar Azucena ◽  
Joel Kubby
Keyword(s):  
Adaptive Optics ◽  
Wavefront Sensing ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals High-resolution two-photon transcranial imaging of brain using direct wavefront sensing

2020 ◽  
Author(s):  
Congping Chen ◽  
Zhongya Qin ◽  
Sicong He ◽  
Shaojun Liu ◽  
Shun-Fat Lau ◽  
...  
Keyword(s):  
High Resolution ◽  
Adaptive Optics ◽  
Pyramidal Neurons ◽  
Imaging System ◽  
Superficial Layer ◽  
Wavefront Sensing ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Transcranial Imaging

AbstractImaging of the brain in its native state at high resolution poses major challenges to visualization techniques. Two-photon microscopy integrated with the thinned-skull or optical clearing skull technique provides a minimally invasive tool for in vivo imaging of the cortex of mice without activating immune response and inducing brain injury. However, the imaging contrast and resolution are severely compromised by the optical heterogeneity of the skull, limiting the imaging depth to the superficial layer. Here, we develop adaptive optics two-photon microscopy for high-resolution transcranial imaging of layer 5 pyramidal neurons up to 700 μm below pia in living mice. In particular, an optimized configuration of imaging system and new wavefront sensing algorithm are proposed for accurate correction for the aberrations induced by the skull window and brain tissue. We investigated microglia-plaque interaction in living brain of Alzheimer’s disease and demonstrated high-precision laser dendrotomy and single-spine ablation.

scholarly journals Multidither coherent optical adaptive technique for deep tissue two-photon microscopy

2019 ◽  
Vol 12 (04) ◽  
pp. 1942003 ◽  
Author(s):  
Biwei Zhang ◽  
Wei Gong ◽  
Chenxue Wu ◽  
Lejia Hu ◽  
Xinpei Zhu ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
Diffraction Theory ◽  
Fluorescence Signal ◽  
Two Photon Microscopy ◽  
Scalar Diffraction ◽  
Two Photon ◽  
Scalar Diffraction Theory ◽  
Adaptive Technique ◽  
Photon Imaging ◽  
Two Photon Imaging

Two-photon microscopy normally suffers from the scattering of the tissue in biological imaging. Multidither coherent optical adaptive technique (COAT) can correct the scattered wavefront in parallel. However, the determination of the corrective phases may not be completely accurate using conventional method, which undermines the performance of this technique. In this paper, we theoretically demonstrate a method that can obtain more accurate corrective phases by determining the phase values from the square root of the fluorescence signal. A numerical simulation model is established to study the performance of adaptive optics in two-photon microscopy by combining scalar diffraction theory with vector diffraction theory. The results show that the distortion of the wavefront can be corrected more thoroughly with our method in two-photon imaging. In our simulation, with the scattering from a 450-[Formula: see text]m-thick mouse brain tissue, excitation focal spots with higher peak-to-background ratio (PBR) and images with higher contrast can be obtained. Hence, further enhancement of the multidither COAT correction performance in two-photon imaging can be expected.

scholarly journals Axial resolution improvement of two-photon microscopy by multi-frame reconstruction and adaptive optics

2020 ◽  
Vol 11 (11) ◽  
pp. 6634
Author(s):  
Shiwei Ye ◽  
Yixuan Yin ◽  
Jing Yao ◽  
Jun Nie ◽  
Yuchen Song ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
Axial Resolution ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Resolution Improvement

Aberration-free 3D imaging via DMD-based two-photon microscopy and sensorless adaptive optics

2020 ◽  
Vol 45 (9) ◽  
pp. 2656 ◽  
Author(s):  
Mindan Ren ◽  
Jialong Chen ◽  
Dihan Chen ◽  
Shih-Chi Chen
Keyword(s):  
Adaptive Optics ◽  
3D Imaging ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals Adaptive optics two-photon microscopy enables near-diffraction-limited and functional retinal imaging in vivo

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Zhongya Qin ◽  
Sicong He ◽  
Chao Yang ◽  
Jasmine Sum-Yee Yung ◽  
Congping Chen ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
Retinal Imaging ◽  
Two Photon Microscopy ◽  
Two Photon

Pulse front adaptive optics in two-photon microscopy

2015 ◽  
Vol 40 (21) ◽  
pp. 4999 ◽  
Author(s):  
Bangshan Sun ◽  
Patrick S. Salter ◽  
Martin J. Booth
Keyword(s):  
Adaptive Optics ◽  
Pulse Front ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals Image-based adaptive optics for two-photon microscopy

2009 ◽  
Vol 34 (16) ◽  
pp. 2495 ◽  
Author(s):  
Delphine Débarre ◽  
Edward J. Botcherby ◽  
Tomoko Watanabe ◽  
Shankar Srinivas ◽  
Martin J. Booth ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
Two Photon Microscopy ◽  
Two Photon
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