Interphase fluorescence in situ hybridization signal detection by computing intensity variance along the optical axis

2014 ◽  
Author(s):  
Zheng Li ◽  
Bin Zheng ◽  
Liqiang Ren ◽  
Hong Liu
Keyword(s):  
In Situ Hybridization ◽  
Signal Detection ◽  
Fluorescence In Situ Hybridization ◽  
Optical Axis ◽  
Hybridization Signal

scholarly journals Evidence That the Caudal Brainstem Is a Target for the Inhibitory Effect of Leptin on Food Intake

Endocrinology ◽  
2002 ◽  
Vol 143 (1) ◽  
pp. 239-246 ◽  
Author(s):  
Harvey J. Grill ◽  
Michael W. Schwartz ◽  
Joel M. Kaplan ◽  
James S. Foxhall ◽  
John Breininger ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Food Intake ◽  
Fluorescence In Situ Hybridization ◽  
Energy Homeostasis ◽  
Leptin Receptor ◽  
Neuronal Cell ◽  
Locus Ceruleus ◽  
Hybridization Signal ◽  
Dorsal Vagal Complex

Abstract Three experiments were performed to investigate the hypothesis that leptin action within the caudal brain stem (CBS) contributes to its intake inhibitory effects. The first experiment evaluated the anatomical distribution of leptin receptor mRNA in rat CBS using a sensitive fluorescence in situ hybridization method with a riboprobe specific for the long form of the leptin receptor (Ob-Rb). An Ob-Rb mRNA hybridization signal was detected in neurons of several CBS nuclei involved in the control of food intake, including the dorsal vagal complex and parabrachial nucleus. A strong hybridization signal was also obtained from neuronal cell bodies of a number of other structures including the hypoglossal, trigeminal, lateral reticular, and cochlear nuclei; locus ceruleus; and inferior olive. The anatomical profile revealed by fluorescence in situ hybridization was in good agreement with immunocytochemical analysis with an antibody specific to Ob-Rb. In a second experiment, exploring the relevance of CBS Ob-Rb to feeding behavior, rats were given a fourth intracerebroventricular (i.c.v.) injection of leptin (0.1, 0.83, or 5.0μ g; n = 9–11/group) or vehicle 30 min before lights-out on three consecutive days The two higher doses reduced food intake significantly at 2, 4, and 24 h after injection and caused significant reductions of body weight. The dose-response profiles for fourth i.c.v. administration were indistinguishable from those obtained from separate groups of rats that received leptin via a lateral i.c.v. cannula. In the last experiment, a ventricle-subthreshold dose of leptin (0.1 μg) microinjected unilaterally into the dorsal vagal complex suppressed food intake at 2, 4, and 24 h. The results indicate that the CBS contains neurons that are potentially direct targets for the action of leptin in the control of energy homeostasis.

scholarly journals Analysis of HER2 Gene Amplification Using an Automated Fluorescence in Situ Hybridization Signal Enumeration System

2007 ◽  
Vol 9 (2) ◽  
pp. 144-150 ◽  
Author(s):  
Rachel Stevens ◽  
Imad Almanaseer ◽  
Miguel Gonzalez ◽  
Derin Caglar ◽  
Ryan A. Knudson ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Gene Amplification ◽  
Hybridization Signal ◽  
Her2 Gene ◽  
Her2 Gene Amplification

890: Multiple Fluorescence in Situ Hybridization (FISH) Probes Increase Sensitivity for Detection of Bladder Cancer

2006 ◽  
Vol 175 (4S) ◽  
pp. 287-288 ◽  
Author(s):  
Juliann M. Dziubinski ◽  
Michael F. Sarosdy ◽  
Paul R. Kahn ◽  
Mark D. Ziffer ◽  
William R. Love ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization

588: Detection of Chromosome 8 Alterations in Paired Needle Biopsy and Prostatectomy Specimens Using Fluorescence In-Situ Hybridization

2004 ◽  
Vol 171 (4S) ◽  
pp. 156-156
Author(s):  
Chandler D. Dora ◽  
Yasushi Kondo ◽  
Fusheng X. Lan ◽  
Jeffrey M. Slezak ◽  
Erik J. Bergstralh ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Needle Biopsy ◽  
Chromosome 8
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