Bi-modal imaging of atherosclerotic plaques: Automated method for co-registration between fluorescence lifetime imaging and intravascular ultrasound data

2014 ◽  
Author(s):  
Dimitris Gorpas ◽  
Hussain Fatakdawala ◽  
Julien Bec ◽  
Dinglong Ma ◽  
Diego R. Yankelevich ◽  
...  
Keyword(s):  
Intravascular Ultrasound ◽  
Fluorescence Lifetime ◽  
Atherosclerotic Plaques ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
Automated Method

scholarly journals Fluorescence lifetime imaging for the characterization of the biochemical composition of atherosclerotic plaques

2011 ◽  
Vol 16 (9) ◽  
pp. 096018 ◽  
Author(s):  
Jennifer Phipps ◽  
Yinghua Sun ◽  
Ramez Saroufeem ◽  
Nisa Hatami ◽  
Michael C. Fishbein ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Biochemical Composition ◽  
Atherosclerotic Plaques ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging

scholarly journals Fluorescence Lifetime Imaging and Intravascular Ultrasound: Co-Registration Study Using Ex Vivo Human Coronaries

2015 ◽  
Vol 34 (1) ◽  
pp. 156-166 ◽  
Author(s):  
Dimitris Gorpas ◽  
Hussain Fatakdawala ◽  
Julien Bec ◽  
Dinglong Ma ◽  
Diego R. Yankelevich ◽  
...  
Keyword(s):  
Intravascular Ultrasound ◽  
Fluorescence Lifetime ◽  
Ex Vivo ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging

Abstract 15508: Random Forest Classifier-incoporated Intravascular Optical Coherence Tomography-fluorescence Lifetime Imaging (oct-flim) Provides Automated Characterization of Key Biochemical Components of Coronary Atherosclerotic Plaques

Circulation ◽  
2020 ◽  
Vol 142 (Suppl_3) ◽  
Author(s):  
Sunwon Kim ◽  
Hyeong Soo Nam ◽  
Woo Jae Kang ◽  
Joon Woo Song ◽  
Hyun Jung Kim ◽  
...  
Keyword(s):  
High Resolution ◽  
Random Forest ◽  
Fluorescence Lifetime ◽  
Biochemical Characterization ◽  
Random Forest Classifier ◽  
Atherosclerotic Plaques ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
Biochemical Components

Introduction: Fluorescence lifetime imaging (FLIm) allows label-free biochemical characterization of atheroma, however, multispectral FLIm produces massive data throughput making image interpretation problematic. We investigated whether machine learning algorithm can be applied to intravascular OCT-FLIm for automated biochemical characterization of coronary plaques. Methods and Results: We built a fully-integrated, high-speed OCT-FLIm system and a low-profile, dual-modal imaging catheter that can provide high-resolution OCT images and correctly co-registered multispectral fluorescence lifetime (FL) readouts: ch.1 and ch.2 FL, and FL intensity ratio (ch.2/ch.1). Rapid intracoronary imaging (10-20 mm/s, 100 rps) was safely performed in atheromatous pigs. There were significant differences in FL measurements according to plaque types (high-risk vs. fibrotic: p<0.001). Multispectral FL measurements sampled selectively from histologically-proven plaque components (lipid, macrophage, lipid+macrophage, SMC) were analyzed. Each component was distinguishable from one another either by difference of FLs or intensity ratio. Random forest classifier (RFC), trained with component-labeled multispectral FL dataset, accurately classified key biochemical components of atherosclerotic plaques. RFC-determined biochemical characteristics of target plaque were consistent across two repeated imaging data (intraclass correlation, p<0.0001) and corroborated closely with those derived from quantitative immunohistochemistries. Conclusions: Our OCT-FLIm incorporating RFC-based systematic multispectral FL analysis could provide high-resolution plaque imaging with automated biochemical characterization in beating coronary environment. The present imaging strategy enabling comprehensive characterization of multiple components of atherosclerotic plaques will open a new avenue in the field of cardiovascular imaging.

scholarly journals Rotational multispectral fluorescence lifetime imaging and intravascular ultrasound: bimodal system for intravascular applications

2014 ◽  
Vol 19 (6) ◽  
pp. 066004 ◽  
Author(s):  
Dinglong Ma ◽  
Julien Bec ◽  
Diego R. Yankelevich ◽  
Dimitris Gorpas ◽  
Hussain Fatakdawala ◽  
...  
Keyword(s):  
Intravascular Ultrasound ◽  
Fluorescence Lifetime ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging

Th-P17:426 Fluorescence lifetime imaging of unstained human atherosclerotic plaques

2006 ◽  
Vol 7 (3) ◽  
pp. 587 ◽  
Author(s):  
L. Hegyi ◽  
C. Talbot ◽  
C. Monaco ◽  
A. Sandison ◽  
A.H. Davies ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Atherosclerotic Plaques ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging
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