scholarly journals Wavefront shaping of a Bessel light field enhances light sheet microscopy with scattered light

2014 ◽  
Author(s):  
J. Nylk ◽  
C. Mitchell ◽  
T. Vettenburg ◽  
F. J. Gunn-Moore ◽  
K. Dholakia
Keyword(s):  
Light Field ◽  
Scattered Light ◽  
Light Sheet ◽  
Light Sheet Microscopy ◽  
Wavefront Shaping

scholarly journals Light-Sheet Microscopy for Surface Topography Measurements and Quantitative Analysis

Sensors ◽  
2020 ◽  
Vol 20 (10) ◽  
pp. 2842 ◽  
Author(s):  
Zhanpeng Xu ◽  
Erik Forsberg ◽  
Yang Guo ◽  
Fuhong Cai ◽  
Sailing He
Keyword(s):  
Surface Topography ◽  
Spatial Resolution ◽  
Scattered Light ◽  
Three Dimensional ◽  
Light Sheet ◽  
Field Of View ◽  
Large Field ◽  
Linear Calibration ◽  
Industrial Sample ◽  
Light Sheet Microscopy

A novel light-sheet microscopy (LSM) system that uses the laser triangulation method to quantitatively calculate and analyze the surface topography of opaque samples is discussed. A spatial resolution of at least 10 μm in z-direction, 10 μm in x-direction and 25 μm in y-direction with a large field-of-view (FOV) is achieved. A set of sample measurements that verify the system′s functionality in various applications are presented. The system has a simple mechanical structure, such that the spatial resolution is easily improved by replacement of the objective, and a linear calibration formula, which enables convenient system calibration. As implemented, the system has strong potential for, e.g., industrial sample line inspections, however, since the method utilizes reflected/scattered light, it also has the potential for three-dimensional analysis of translucent and layered structures.

scholarly journals A Hybrid of Light-Field and Light-Sheet Imaging to Decouple Myocardial Biomechanics from Intracardiac Flow Dynamics

2020 ◽  
Author(s):  
Zhaoqiang Wang ◽  
Yichen Ding ◽  
Sandro Satta ◽  
Mehrdad Roustaei ◽  
Peng Fei ◽  
...  
Keyword(s):  
Blood Flow ◽  
Blood Cells ◽  
Myocardial Contractility ◽  
Light Field ◽  
Cardiac Mechanics ◽  
Light Sheet ◽  
Flow Dynamics ◽  
Three Dimensions ◽  
Light Sheet Microscopy ◽  
Intracardiac Flow

AbstractBiomechanical forces intimately contribute to cardiac morphogenesis. However, 4-D (3-D space + time) imaging is needed to investigate the developmental cardiac mechanics with high temporal and spatial resolution. We hereby integrated light-sheet fluorescence microscopy (LSFM) with light-field microscopy (LFM), to simultaneously visualize myocardial contractility and intracardiac blood flow in three dimensions at 200 volumes per second (vps). LSFM allows for reconstruction of the myocardial contraction in zebrafish embryo; and LFM enables simultaneous tracking of the blood cells entering and leaving the contracting heart. We herein established particle tracking velocimetry to interrogate the trajectories of intracardiac blood cells, and we demonstrated deformable image registration to reveal a decrease in the myocardial contractility from atrioventricular (AV) canal to the outflow tract (OFT). We imaged myocardium undergoing torsional contraction and blood flow undergoing regurgitation. Taken together, the integration of light-field and light-sheet microscopy, followed by an image-based analysis pipeline, provides the biomechanical insights into coupling myocardial kinetics with rotational contraction along with intracardiac flow dynamics during development.

scholarly journals Enhanced Light Sheet Elastic Scattering Microscopy by Using a Supercontinuum Laser

2019 ◽  
Vol 2 (3) ◽  
pp. 57 ◽  
Author(s):  
Diego Di Battista ◽  
David Merino ◽  
Giannis Zacharakis ◽  
Pablo Loza-Alvarez ◽  
Omar E. Olarte
Keyword(s):  
Elastic Scattering ◽  
Spatial Scales ◽  
Scattered Light ◽  
Spatial Coherence ◽  
Light Sheet ◽  
Structural Features ◽  
Light Sheet Microscopy ◽  
Excitation Laser ◽  
Polarization Filtering ◽  
Microscopy Techniques

Light sheet fluorescence microscopy techniques have revolutionized biological microscopy enabling low-phototoxic long-term 3D imaging of living samples. Although there exist many light sheet microscopy (LSM) implementations relying on fluorescence, just a few works have paid attention to the laser elastic scattering source of contrast available in every light sheet microscope. Interestingly, elastic scattering can potentially disclose valuable information from the structure and composition of the sample at different spatial scales. However, when coherent scattered light is detected with a camera sensor, a speckled intensity is generated on top of the native imaged features, compromising their visibility. In this work, we propose a novel light sheet based optical setup which implements three strategies for dealing with speckles of elastic scattering images: (i) polarization filtering; (ii) reducing the temporal coherence of the excitation laser light; and, (iii) reducing the spatial coherence of the light sheet. Finally, we show how these strategies enable pristine light-sheet elastic-scattering imaging of structural features in challenging biological samples avoiding the deleterious effects of speckle, and without relying on, but complementing, fluorescent labelling.

scholarly journals Protocol for the Design and Assembly of a Light Sheet Light Field Microscope

2019 ◽  
Vol 2 (3) ◽  
pp. 56 ◽  
Author(s):  
Jorge Madrid-Wolff ◽  
Manu Forero-Shelton
Keyword(s):  
Neural Activity ◽  
Light Field ◽  
Signal To Noise Ratio ◽  
Light Sheet ◽  
High Temporal Resolution ◽  
Single Camera ◽  
Signal To Noise ◽  
Light Sheet Microscopy ◽  
Recent Method ◽  
Noise Ratio

Light field microscopy is a recent development that makes it possible to obtain images of volumes with a single camera exposure, enabling studies of fast processes such as neural activity in zebrafish brains at high temporal resolution, at the expense of spatial resolution. Light sheet microscopy is also a recent method that reduces illumination intensity while increasing the signal-to-noise ratio with respect to confocal microscopes. While faster and gentler to samples than confocals for a similar resolution, light sheet microscopy is still slower than light field microscopy since it must collect volume slices sequentially. Nonetheless, the combination of the two methods, i.e., light field microscopes that have light sheet illumination, can help to improve the signal-to-noise ratio of light field microscopes and potentially improve their resolution. Building these microscopes requires much expertise, and the resources for doing so are limited. Here, we present a protocol to build a light field microscope with light sheet illumination. This protocol is also useful to build a light sheet microscope.

scholarly journals Multidirectional digital scanned light-sheet microscopy enables uniform fluorescence excitation and contrast-enhanced imaging

2018 ◽  
Author(s):  
Adam K. Glaser ◽  
Ye Chen ◽  
Chengbo Yin ◽  
Linpeng Wei ◽  
Lindsey A. Barner ◽  
...  
Keyword(s):  
Scattered Light ◽  
Light Sheet ◽  
Line Detection ◽  
Fluorescence Excitation ◽  
Light Sheet Microscopy ◽  
Contrast Enhanced ◽  
Selective Plane Illumination Microscopy ◽  
Light Sheet Fluorescence Microscopy ◽  
Enhanced Imaging ◽  
Imaging Plane

AbstractLight-sheet fluorescence microscopy (LSFM) has emerged as a powerful method for rapid and optically efficient 3D microscopy. Initial LSFM designs utilized a static sheet of light, termed selective plane illumination microscopy (SPIM), which exhibited shadowing artifacts and deteriorated contrast due to light scattering. These issues have been addressed, in part, by multidirectional selective plane illumination microscopy (mSPIM), in which rotation of the light sheet is used to mitigate shadowing artifacts, and digital scanned light-sheet microscopy (DSLM), in which confocal line detection is used to reject scattered light. Here we present a simple passive multidirectional digital scanned light-sheet microscopy (mDSLM) architecture that combines the benefits of mSPIM and DSLM. By utilizing an elliptical Gaussian beam with increased angular diversity in the imaging plane, mDSLM provides shadow-free contrast-enhanced imaging of fluorescently labeled samples.One Sentence SummaryGlaser et al. describe a light-sheet microscopy architecture that enables passive multidirectional illumination with confocal line detection to enable both uniform fluorescence excitation and contrast-enhanced imaging of fluorescently labeled samples.

scholarly journals Achromatic terahertz Airy beam generation with dielectric metasurfaces

Nanophotonics ◽  
2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Qingqing Cheng ◽  
Juncheng Wang ◽  
Ling Ma ◽  
Zhixiong Shen ◽  
Jing Zhang ◽  
...  
Keyword(s):  
Biomedical Imaging ◽  
Frequency Dispersion ◽  
Light Sheet ◽  
Photonic Devices ◽  
Self Healing ◽  
Beam Focusing ◽  
Light Sheet Microscopy ◽  
Airy Beam ◽  
Potential Applications ◽  
Airy Beams

AbstractAiry beams exhibit intriguing properties such as nonspreading, self-bending, and self-healing and have attracted considerable recent interest because of their many potential applications in photonics, such as to beam focusing, light-sheet microscopy, and biomedical imaging. However, previous approaches to generate Airy beams using photonic structures have suffered from severe chromatic problems arising from strong frequency dispersion of the scatterers. Here, we design and fabricate a metasurface composed of silicon posts for the frequency range 0.4–0.8 THz in transmission mode, and we experimentally demonstrate achromatic Airy beams exhibiting autofocusing properties. We further show numerically that a generated achromatic Airy-beam-based metalens exhibits self-healing properties that are immune to scattering by particles and that it also possesses a larger depth of focus than a traditional metalens. Our results pave the way to the realization of flat photonic devices for applications to noninvasive biomedical imaging and light-sheet microscopy, and we provide a numerical demonstration of a device protocol.

scholarly journals Single-Molecule Light-Sheet Microscopy with Local Nanopipette Delivery

2021 ◽  
Vol 93 (8) ◽  
pp. 4092-4099
Author(s):  
Bing Li ◽  
Aleks Ponjavic ◽  
Wei-Hsin Chen ◽  
Lee Hopkins ◽  
Craig Hughes ◽  
...  
Keyword(s):  
Single Molecule ◽  
Light Sheet ◽  
Light Sheet Microscopy

scholarly journals Effect of captopril on post-infarction remodelling visualized by light sheet microscopy and echocardiography

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Urmas Roostalu ◽  
Louise Thisted ◽  
Jacob Lercke Skytte ◽  
Casper Gravesen Salinas ◽  
Philip Juhl Pedersen ◽  
...  
Keyword(s):  
Morphological Changes ◽  
Light Sheet ◽  
Automated Image Analysis ◽  
Border Zone ◽  
Vascular Density ◽  
Mouse Heart ◽  
Imaging Method ◽  
Light Sheet Microscopy ◽  
Post Surgery ◽  
Captopril Treatment

AbstractAngiotensin converting enzyme inhibitors, among them captopril, improve survival following myocardial infarction (MI). The mechanisms of captopril action remain inadequately understood due to its diverse effects on multiple signalling pathways at different time periods following MI. Here we aimed to establish the role of captopril in late-stage post-MI remodelling. Left anterior descending artery (LAD) ligation or sham surgery was carried out in male C57BL/6J mice. Seven days post-surgery LAD ligated mice were allocated to daily vehicle or captopril treatment continued over four weeks. To provide comprehensive characterization of the changes in mouse heart following MI a 3D light sheet imaging method was established together with automated image analysis workflow. The combination of echocardiography and light sheet imaging enabled to assess cardiac function and the underlying morphological changes. We show that delayed captopril treatment does not affect infarct size but prevents left ventricle dilation and hypertrophy, resulting in improved ejection fraction. Quantification of lectin perfused blood vessels showed improved vascular density in the infarct border zone in captopril treated mice in comparison to vehicle dosed control mice. These results validate the applicability of combined echocardiographic and light sheet assessment of drug mode of action in preclinical cardiovascular research.

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