High-sensitivity single molecule fluorescence detection using scanning single-molecule counting

2014 ◽  
Author(s):  
Mitsushiro Yamaguchi ◽  
Tetsuya Tanabe ◽  
Hidetaka Nakata ◽  
Takuya Hanashi ◽  
Kazutaka Nishikawa ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Single Molecule ◽  
High Sensitivity ◽  
Single Molecule Fluorescence

scholarly journals Single-Molecule Fluorescence Detection of a Synthetic Heparan Sulfate Disaccharide

ChemPhysChem ◽  
2016 ◽  
Vol 17 (21) ◽  
pp. 3442-3446 ◽  
Author(s):  
Charlotte E. Dalton ◽  
Steven D. Quinn ◽  
Aidan Rafferty ◽  
Michael J. Morten ◽  
John M. Gardiner ◽  
...  
Keyword(s):  
Heparan Sulfate ◽  
Fluorescence Detection ◽  
Single Molecule ◽  
Single Molecule Fluorescence

Nucleic acid amplification-integrated single-molecule fluorescence imaging for in vitro and in vivo biosensing

2021 ◽  
Author(s):  
Fei Ma ◽  
Chen-Chen Li ◽  
Chun-Yang Zhang
Keyword(s):  
Nucleic Acid ◽  
Fluorescence Imaging ◽  
Single Molecule ◽  
High Sensitivity ◽  
Nucleic Acid Amplification ◽  
Single Molecule Fluorescence

Single-molecule fluorescence imaging is among the most advanced analytical technologies and has been widely adopted for biosensing due to its distinct advantages of simplicity, rapidity, high sensitivity, low sample consumption,...

scholarly journals Probing DNA ‐ Transcription Factor Interactions Using Single‐Molecule Fluorescence Detection in Nanofluidic Devices

2021 ◽  
pp. 2100953
Author(s):  
Mattia Fontana ◽  
Šarunė Ivanovaitė ◽  
Simon Lindhoud ◽  
Elmar van der Wijk ◽  
Klaus Mathwig ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Fluorescence Detection ◽  
Single Molecule ◽  
Single Molecule Fluorescence ◽  
Dna Transcription ◽  
Nanofluidic Devices ◽  
Factor Interactions

scholarly journals Systematic Assessment of Burst Impurity in Confocal-Based Single-Molecule Fluorescence Detection Using Brownian Motion Simulations

Molecules ◽  
2019 ◽  
Vol 24 (14) ◽  
pp. 2557 ◽  
Author(s):  
Dolev Hagai ◽  
Eitan Lerner
Keyword(s):  
Brownian Motion ◽  
Fluorescence Detection ◽  
Single Molecule ◽  
Three Dimensional ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Single Molecule Fluorescence ◽  
Experimental Conditions ◽  
Systematic Assessment ◽  
Burst Analysis

Single-molecule fluorescence detection (SMFD) experiments are useful in distinguishing sub-populations of molecular species when measuring heterogeneous samples. One experimental platform for SMFD is based on a confocal microscope, where molecules randomly traverse an effective detection volume. The non-uniformity of the excitation profile and the random nature of Brownian motion, produce fluctuating fluorescence signals. For these signals to be distinguished from the background, burst analysis is frequently used. Yet, the relation between the results of burst analyses and the underlying information of the diffusing molecules is still obscure and requires systematic assessment. In this work we performed three-dimensional Brownian motion simulations of SMFD, and tested the positions at which molecules emitted photons that passed the burst analysis criteria for different values of burst analysis parameters. The results of this work verify which of the burst analysis parameters and experimental conditions influence both the position of molecules in space when fluorescence is detected and taken into account, and whether these bursts of photons arise purely from single molecules, or not entirely. Finally, we show, as an example, the effect of bursts that are not purely from a single molecule on the accuracy in single-molecule Förster resonance energy transfer measurements.

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