Influence of serum proteins on the accumulation of aminolaevulinic acid-induced protoporphyrin IX in cells in culture

1994 ◽  
Author(s):  
M. M. Weir ◽  
David I. Vernon ◽  
Stanley B. Brown
Keyword(s):  
Serum Proteins ◽  
Protoporphyrin Ix ◽  
Cells In Culture ◽  
Aminolaevulinic Acid ◽  
In Cells

scholarly journals Factors affecting aminolaevulinic acid-induced generation of protoporphyrin IX

1997 ◽  
Vol 76 (6) ◽  
pp. 705-712 ◽  
Author(s):  
L Wyld ◽  
JL Burn ◽  
MWR Reed ◽  
NJ Brown
Keyword(s):  
Protoporphyrin Ix ◽  
Factors Affecting ◽  
Aminolaevulinic Acid

scholarly journals Photodynamic detection of a feline meningioma using 5-aminolaevulinic acid hydrochloride

2020 ◽  
Vol 6 (1) ◽  
pp. 205511692090742
Author(s):  
Tomohiro Osaki ◽  
Kengo Gonda ◽  
Yusuke Murahata ◽  
Yuji Sunden ◽  
Takao Amaha ◽  
...  
Keyword(s):  
Brain Tumours ◽  
High Performance ◽  
Protein Concentration ◽  
Protoporphyrin Ix ◽  
Aminolaevulinic Acid ◽  
Case Summary ◽  
Axial Mass ◽  
Acid Hydrochloride ◽  
Human Brain Tumours ◽  
Spectrum Evaluation

Case summary The present study describes the case of a feline meningioma that was detected using 5-aminolaevulinic acid hydrochloride (5-ALA)-induced protoporphyrin IX (PpIX) fluorescence at surgery. An extra-axial mass in the temporoparietal region was observed by MRI. Following craniectomy and durotomy, photodynamic detection (PDD) was performed for detection of the tumour. Intratumour PpIX was detected using fluorescence spectrum evaluation and high-performance liquid chromatography. PDD revealed bright fluorescence of PpIX induced by 5-ALA, facilitating fluorescence-guided resection of the tumour tissue. Postoperative examination demonstrated an intratumour PpIX protein concentration of 16.8 nmol/g, and based on histopathological findings we diagnosed the mass as meningioma. Relevance and novel information PDD using 5-ALA has been used to identify the surgical margins during resection of primary human brain tumours. Recently, we have reported post-mortem PDD using 5-ALA for a canine glioblastoma. To our knowledge, this technique has not been previously used for the detection and resection of feline brain tumours. Our findings suggest that PDD using 5-ALA is useful for intraoperative fluorescence-guided resection of malignant meningioma in cats.

scholarly journals Molecular cloning and characterization of a transcription factor for the copia retrotransposon with homology to the BTB-containing lola neurogenic factor.

1997 ◽  
Vol 17 (1) ◽  
pp. 482-494 ◽  
Author(s):  
L Cavarec ◽  
S Jensen ◽  
J F Casella ◽  
S A Cristescu ◽  
T Heidmann
Keyword(s):  
Transcription Factor ◽  
Transcriptional Activation ◽  
Axon Growth ◽  
Expression Vectors ◽  
Recognition Sequence ◽  
Enhancer Sequence ◽  
Cells In Culture ◽  
Copia Retrotransposon ◽  
Gel Shift ◽  
In Cells

By transfection experiments, we previously identified a 72-bp enhancer sequence within the Drosophila copia retrotransposon which is involved in the control of the transcription level of this mobile element in cells in culture. Gel shift assays with nuclear extracts from Drosophila hydei-derived DH-33 cells further demonstrated specific interactions of at least two nuclear factors with this enhancer sequence. Using this sequence as a probe for the screening of an expression cDNA library that we constructed from DH-33 cells RNA, we have isolated a cDNA clone encoding a 110-kDa protein with features common to those of known transcription factors; these include a two-zinc-finger motif at the C terminus, three glutamine-rich domains in the presumptive activation domain of the protein, and an N-terminal domain which shares homology with the Bric-à-brac, Tramtrack, and Broad-Complex BTB boxes. The precise DNA recognition sequence for this transcription factor has been determined by both gel shift assays and footprinting experiments with a recombinant protein made in bacteria. The functionality of the cloned element was demonstrated upon transcriptional activation of copia reporter genes, as well as of a minimal promoter coupled with the identified target DNA sequence, in cotransfection assays in cells in culture with an expression vector for the cloned factor. Southern blot and nucleotide sequence analyses revealed a related gene in Drosophila melanogaster (the lola gene) previously identified by a genetic approach as involved in axon growth and guidance. Transfection assays in cells in culture with lola gene expression vectors and in situ hybridization experiments with lola gene mutants finally provided evidence that the copia retrotransposon is regulated by this neurogenic gene in D.melanogaster, with a repressor effect in the central nervous systems of the embryos.

scholarly journals The reconstitution of oxidase activity in membranes derived from a 5-aminolaevulinic acid-requiring mutant of Escherichia coli

1973 ◽  
Vol 136 (4) ◽  
pp. 877-884 ◽  
Author(s):  
Bruce A. Haddock
Keyword(s):  
Escherichia Coli ◽  
Electron Transport ◽  
Cytochrome B ◽  
Oxidase Activity ◽  
Nadh Dehydrogenase ◽  
Carbon Sources ◽  
Protoporphyrin Ix ◽  
Side Chain ◽  
E Coli ◽  
Aminolaevulinic Acid

1. The reconstitution of oxidase activity in cell-free extracts of a mutant of Escherichia coli K12Ymel, that require 5-aminolaevulinic acid for growth on non-fermentable carbon sources, is described. 2. The reconstitution is dependent on haematin or a haem extract from a prototrophic strain of E. coli, and the product of the reaction has been identified as NADH-reducible cytochrome b. 3. The requirement for haematin cannot be replaced by four other porphyrins. Coproporphyrin III does not inhibit the haematin-dependent reconstitution, mesoporphyrin IX and protoporphyrin IX apparently compete with haematin for a binding site on the cytochrome apoprotein(s) and deuteroporphyrin IX binds to cytochrome apoprotein(s) and cannot be subsequently replaced by haematin. 4. The properties of electron-transport particles from cell-free extracts of the mutant strain, grown aerobically in the presence or absence of 5-aminolaevulinic acid, are described. In the absence of 5-aminolaevulinic acid no detectable cytochromes are produced, and oxidase activities are lowered but there is no apparent effect on the activities of the NADH dehydrogenase and d-lactate dehydrogenase. 5. The reconstitution of oxidase activity by electron-transport particles from cells grown in the absence of 5-aminolaevulinic acid requires ATP and haematin, and the product of the reaction was identified as NADH-reducible cytochrome b. 6. It is concluded that the cytochrome apoproteins are synthesized and incorporated into the cytoplasmic membrane of E. coli in the absence of haem synthesis. The subsequent reconstitution of functional cytochrome(s) requires protohaem, but the nature of the side chain on the 2 and 4 positions of the porphyrin appears to be important.

Sign in / Sign up

Export Citation Format

Share Document