Investigation of mechanical property of a cell by optical tweezers

2013 ◽  
Author(s):  
T. Sugiura
Keyword(s):  
Mechanical Property ◽  
Optical Tweezers ◽  
A Cell

scholarly journals Conformation and mechanical property of rpoS mRNA inhibitory stem studied by optical tweezers and X-ray scattering

PLoS ONE ◽  
2019 ◽  
Vol 14 (9) ◽  
pp. e0222938
Author(s):  
Xinyao Hu ◽  
Xuanling Li ◽  
Lingna Yang ◽  
Yilin Zhu ◽  
Yunyu Shi ◽  
...  
Keyword(s):  
Mechanical Property ◽  
Optical Tweezers ◽  
X Ray ◽  
X Ray Scattering ◽  
Rpos Mrna ◽  
Ray Scattering

scholarly journals A cell-laden nanofiber/hydrogel composite structure with tough-soft mechanical property

2013 ◽  
Vol 102 (21) ◽  
pp. 211914 ◽  
Author(s):  
Jinah Jang ◽  
Hana Oh ◽  
Jongwan Lee ◽  
Tae-Ha Song ◽  
Young Hun Jeong ◽  
...  
Keyword(s):  
Mechanical Property ◽  
Composite Structure ◽  
Hydrogel Composite ◽  
A Cell

Measuring local properties inside a cell‐mimicking structure using rotating optical tweezers

2019 ◽  
Vol 12 (7) ◽  
Author(s):  
Shu Zhang ◽  
Lachlan J. Gibson ◽  
Alexander B. Stilgoe ◽  
Timo A. Nieminen ◽  
Halina Rubinsztein‐Dunlop
Keyword(s):  
Optical Tweezers ◽  
A Cell ◽  
Local Properties

scholarly journals Force and Stepwise Movements of Gliding Motility in Human Pathogenic Bacterium Mycoplasma pneumoniae

2021 ◽  
Vol 12 ◽  
Author(s):  
Masaki Mizutani ◽  
Yuya Sasajima ◽  
Makoto Miyata
Keyword(s):  
Optical Tweezers ◽  
Mycoplasma Pneumoniae ◽  
Binding Activity ◽  
Gliding Motility ◽  
Pathogenic Bacterium ◽  
Step Size ◽  
Bacterial Surface ◽  
Human Pathogenic Bacterium ◽  
A Cell ◽  
Stall Force

Mycoplasma pneumoniae, a human pathogenic bacterium, binds to sialylated oligosaccharides and glides on host cell surfaces via a unique mechanism. Gliding motility is essential for initiating the infectious process. In the present study, we measured the stall force of an M. pneumoniae cell carrying a bead that was manipulated using optical tweezers on two strains. The stall forces of M129 and FH strains were averaged to be 23.7 and 19.7 pN, respectively, much weaker than those of other bacterial surface motilities. The binding activity and gliding speed of the M129 strain on sialylated oligosaccharides were eight and two times higher than those of the FH strain, respectively, showing that binding activity is not linked to gliding force. Gliding speed decreased when cell binding was reduced by addition of free sialylated oligosaccharides, indicating the existence of a drag force during gliding. We detected stepwise movements, likely caused by a single leg under 0.2-0.3 mM free sialylated oligosaccharides. A step size of 14-19 nm showed that 25-35 propulsion steps per second are required to achieve the usual gliding speed. The step size was reduced to less than half with the load applied using optical tweezers, showing that a 2.5 pN force from a cell is exerted on a leg. The work performed in this step was 16-30% of the free energy of the hydrolysis of ATP molecules, suggesting that this step is linked to the elementary process of M. pneumoniae gliding. We discuss a model to explain the gliding mechanism, based on the information currently available.

scholarly journals Directed Binding of Gliding Bacterium,Mycoplasma mobile, Shown by Detachment Force and Bond Lifetime

mBio ◽  
2016 ◽  
Vol 7 (3) ◽  
Author(s):  
Akihiro Tanaka ◽  
Daisuke Nakane ◽  
Masaki Mizutani ◽  
Takayuki Nishizaka ◽  
Makoto Miyata
Keyword(s):  
Optical Tweezers ◽  
Atp Hydrolysis ◽  
Critical Role ◽  
Influenza Viruses ◽  
Polystyrene Bead ◽  
Detachment Force ◽  
A Cell ◽  
Gliding Bacterium ◽  
Cellular Protrusion ◽  
Gliding Mechanism

ABSTRACTMycoplasma mobile, a fish-pathogenic bacterium, features a protrusion that enables it to glide smoothly on solid surfaces at a velocity of up to 4.5 µm s−1in the direction of the protrusion.M. mobileglides by a repeated catch-pull-release of sialylated oligosaccharides fixed on a solid surface by hundreds of 50-nm flexible “legs” sticking out from the protrusion. This gliding mechanism may be explained by a possible directed binding of each leg with sialylated oligosaccharides, by which the leg can be detached more easily forward than backward. In the present study, we used a polystyrene bead held by optical tweezers to detach a starved cell at rest from a glass surface coated with sialylated oligosaccharides and concluded that the detachment force forward is 1.6- to 1.8-fold less than that backward, which may be linked to a catch bond-like behavior of the cell. These results suggest that this directed binding has a critical role in the gliding mechanism.IMPORTANCEMycoplasmaspecies are the smallest bacteria and are parasitic and occasionally commensal, as represented byMycoplasma pneumoniae, which causes so-called “walking pneumonia” in humans. Dozens of species glide on host tissues, always in the direction of the characteristic cellular protrusion, by novel mechanisms. The fastest species,Mycoplasma mobile, catches, pulls, and releases sialylated oligosaccharides (SOs), which are common targets among influenza viruses, by means of a specific receptor based on the energy of ATP hydrolysis. Here, force measurements made with optical tweezers revealed that the force required to detach a cell from SOs is smaller forward than backward along the gliding direction. The directed binding should be a clue to elucidate this novel motility mechanism.

scholarly journals Tele–Robotic Platform for Dexterous Optical Single-Cell Manipulation

Micromachines ◽  
2019 ◽  
Vol 10 (10) ◽  
pp. 677 ◽  
Author(s):  
Edison Gerena ◽  
Florent Legendre ◽  
Akshay Molawade ◽  
Youen Vitry ◽  
Stéphane Régnier ◽  
...  
Keyword(s):  
Single Cell ◽  
Optical Tweezers ◽  
Biomedical Applications ◽  
High Speed ◽  
Cell Manipulation ◽  
Optical Traps ◽  
A Cell ◽  
Six Dof ◽  
Master Device ◽  
Single Cell Manipulation

Single-cell manipulation is considered a key technology in biomedical research. However, the lack of intuitive and effective systems makes this technology less accessible. We propose a new tele–robotic solution for dexterous cell manipulation through optical tweezers. A slave-device consists of a combination of robot-assisted stages and a high-speed multi-trap technique. It allows for the manipulation of more than 15 optical traps in a large workspace with nanometric resolution. A master-device (6+1 degree of freedom (DoF)) is employed to control the 3D position of optical traps in different arrangements for specific purposes. Precision and efficiency studies are carried out with trajectory control tasks. Three state-of-the-art experiments were performed to verify the efficiency of the proposed platform. First, the reliable 3D rotation of a cell is demonstrated. Secondly, a six-DoF teleoperated optical-robot is used to transport a cluster of cells. Finally, a single-cell is dexterously manipulated through an optical-robot with a fork end-effector. Results illustrate the capability to perform complex tasks in efficient and intuitive ways, opening possibilities for new biomedical applications.

scholarly journals Single-Cell Elasticity Measurement with an Optically Actuated Microrobot

Micromachines ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 882
Author(s):  
István Grexa ◽  
Tamás Fekete ◽  
Judit Molnár ◽  
Kinga Molnár ◽  
Gaszton Vizsnyiczai ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Target Cells ◽  
Lateral Direction ◽  
Two Photon ◽  
Holographic Optical Tweezers ◽  
Feasible Alternative ◽  
A Cell ◽  
Cell Elasticity ◽  
Force Range ◽  
Elasticity Measurement

A cell elasticity measurement method is introduced that uses polymer microtools actuated by holographic optical tweezers. The microtools were prepared with two-photon polymerization. Their shape enables the approach of the cells in any lateral direction. In the presented case, endothelial cells grown on vertical polymer walls were probed by the tools in a lateral direction. The use of specially shaped microtools prevents the target cells from photodamage that may arise during optical trapping. The position of the tools was recorded simply with video microscopy and analyzed with image processing methods. We critically compare the resulting Young’s modulus values to those in the literature obtained by other methods. The application of optical tweezers extends the force range available for cell indentations measurements down to the fN regime. Our approach demonstrates a feasible alternative to the usual vertical indentation experiments.

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