Multispot two-photon imaging of calcium waves dynamics in cardial tissue at 16Hz frame rate

2013 ◽  
Author(s):  
C. de Mauro ◽  
C. A. Cecchetti ◽  
D. Alfieri ◽  
G. Borile ◽  
A. Urbani ◽  
...  
Keyword(s):  
Frame Rate ◽  
Calcium Waves ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging

Detection of calcium waves in mice heart tissue with multispot two-photon imaging

2013 ◽  
Author(s):  
C. de Mauro ◽  
C. A. Cecchetti ◽  
D. Alfieri ◽  
G. Borile ◽  
A. Urbani ◽  
...  
Keyword(s):  
Heart Tissue ◽  
Calcium Waves ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging

Multispot two-photon imaging of mice heart tissue detecting calcium waves

2012 ◽  
Author(s):  
C. de Mauro ◽  
C. A. Cecchetti ◽  
D. Alfieri ◽  
G. Borile ◽  
M. Mongillo ◽  
...  
Keyword(s):  
Heart Tissue ◽  
Calcium Waves ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging

scholarly journals Volumetric Two-photon Imaging of Neurons Using Stereoscopy (vTwINS)

2016 ◽  
Author(s):  
Alexander Song ◽  
Adam S. Charles ◽  
Sue Ann Koay ◽  
Jeff L. Gauthier ◽  
Stephan Y. Thiberge ◽  
...  
Keyword(s):  
Calcium Imaging ◽  
Laser Scanning ◽  
Large Scale ◽  
Laser Scanning Microscopy ◽  
Frame Rate ◽  
Neural Population ◽  
Imaging Method ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging

AbstractTwo-photon laser scanning microscopy of calcium dynamics using fluorescent indicators is a widely used imaging method for large scale recording of neural activity in vivo. Here we introduce volumetric Two-photon Imaging of Neurons using Stereoscopy (vTwINS), a volumetric calcium imaging method that employs an elongated, V-shaped point spread function to image a 3D brain volume. Single neurons project to spatially displaced image pairs in the resulting 2D image, and the separation distance between images is proportional to depth in the volume. To demix the fluorescence time series of individual neurons, we introduce a novel orthogonal matching pursuit algorithm that also infers source locations within the 3D volume. We illustrate vTwINS by imaging neural population activity in mouse primary visual cortex and hippocampus. Our results demonstrate that vTwINS provides an effective method for volumetric two-photon calcium imaging that increases the number of neurons recorded while maintaining a high frame-rate.

Biocompatible well-defined chromophore–polymer conjugates for photodynamic therapy and two-photon imaging

2013 ◽  
Vol 4 (1) ◽  
pp. 61-67 ◽  
Author(s):  
Cristina Cepraga ◽  
Thibault Gallavardin ◽  
Sophie Marotte ◽  
Pierre-Henri Lanoë ◽  
Jean-Christophe Mulatier ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Two Photon ◽  
Polymer Conjugates ◽  
Photon Imaging ◽  
Two Photon Imaging
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