Automated analysis of 3D morphology of human red blood cells via off-axis digital holographic microscopy

2013 ◽  
Author(s):  
Inkyu Moon
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Automated Analysis ◽  
Digital Holographic Microscopy ◽  
Human Red Blood Cells ◽  
3D Morphology ◽  
Holographic Microscopy

Tomography of red blood cells by multiple-wavelength digital holographic microscopy

2009 ◽  
Author(s):  
Jonas Kühn ◽  
Frédéric Montfort ◽  
Tristan Colomb ◽  
Benjamin Rappaz ◽  
Corinne Moratal ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Digital Holographic Microscopy ◽  
Multiple Wavelength ◽  
Holographic Microscopy

scholarly journals Biomechanics of Ex Vivo-Generated Red Blood Cells Investigated by Optical Tweezers and Digital Holographic Microscopy

Cells ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 552
Author(s):  
Claudia Bernecker ◽  
Maria Augusta R. B. F. Lima ◽  
Catalin D. Ciubotaru ◽  
Peter Schlenke ◽  
Isabel Dorn ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Optical Tweezers ◽  
Blood Cells ◽  
Ex Vivo ◽  
Single Cells ◽  
Membrane Lipid ◽  
Membrane Properties ◽  
Digital Holographic Microscopy ◽  
Holographic Microscopy

Ex vivo-generated red blood cells are a promising resource for future safe blood products, manufactured independently of voluntary blood donations. The physiological process of terminal maturation from spheroid reticulocytes to biconcave erythrocytes has not been accomplished yet. A better biomechanical characterization of cultured red blood cells (cRBCs) will be of utmost interest for manufacturer approval and therapeutic application. Here, we introduce a novel optical tweezer (OT) approach to measure the deformation and elasticity of single cells trapped away from the coverslip. To investigate membrane properties dependent on membrane lipid content, two culture conditions of cRBCs were investigated, cRBCPlasma with plasma and cRBCHPL supplemented with human platelet lysate. Biomechanical characterization of cells under optical forces proves the similar features of native RBCs and cRBCHPL, and different characteristics for cRBCPlasma. To confirm these results, we also applied a second technique, digital holographic microscopy (DHM), for cells laid on the surface. OT and DHM provided related results in terms of cell deformation and membrane fluctuations, allowing a reliable discrimination between cultured and native red blood cells. The two techniques are compared and discussed in terms of application and complementarity.

Automated segmentation of multiple red blood cells with digital holographic microscopy

2013 ◽  
Vol 18 (2) ◽  
pp. 026006 ◽  
Author(s):  
Faliu Yi ◽  
Inkyu Moon ◽  
Bahram Javidi ◽  
Daniel Boss ◽  
Pierre Marquet
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Digital Holographic Microscopy ◽  
Automated Segmentation ◽  
Holographic Microscopy
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