Mouse neuroblastoma cell based model and the effect of epileptic events on calcium oscillations and neural spikes

2013 ◽  
Author(s):  
Suhwan Kim ◽  
Juyeong Baek ◽  
Unsang Jung ◽  
Sangwon Lee ◽  
Woonggyu Jung ◽  
...  
Keyword(s):  
Neuroblastoma Cell ◽  
Calcium Oscillations ◽  
Mouse Neuroblastoma Cell ◽  
Neural Spikes ◽  
Mouse Neuroblastoma

scholarly journals Species-Independent Inhibition of Abnormal Prion Protein (PrP) Formation by a Peptide Containing a Conserved PrP Sequence

1999 ◽  
Vol 73 (8) ◽  
pp. 6245-6250 ◽  
Author(s):  
Joëlle Chabry ◽  
Suzette A. Priola ◽  
Kathy Wehrly ◽  
Jane Nishio ◽  
James Hope ◽  
...  
Keyword(s):  
Prion Protein ◽  
Transmissible Spongiform Encephalopathy ◽  
Neuroblastoma Cell ◽  
Spongiform Encephalopathy ◽  
Inhibitory Effects ◽  
Mouse Neuroblastoma Cell ◽  
Mouse Neuroblastoma ◽  
Scrapie Strain ◽  
Species Specific ◽  
Scrapie Strains

ABSTRACT Conversion of the normal protease-sensitive prion protein (PrP) to its abnormal protease-resistant isoform (PrP-res) is a major feature of the pathogenesis associated with transmissible spongiform encephalopathy (TSE) diseases. In previous experiments, PrP conversion was inhibited by a peptide composed of hamster PrP residues 109 to 141, suggesting that this region of the PrP molecule plays a crucial role in the conversion process. In this study, we used PrP-res derived from animals infected with two different mouse scrapie strains and one hamster scrapie strain to investigate the species specificity of these conversion reactions. Conversion of PrP was found to be completely species specific; however, despite having three amino acid differences, peptides corresponding to the hamster and mouse PrP sequences from residues 109 to 141 inhibited both the mouse and hamster PrP conversion systems equally. Furthermore, a peptide corresponding to hamster PrP residues 119 to 136, which was identical in both mouse and hamster PrP, was able to inhibit PrP-res formation in both the mouse and hamster cell-free systems as well as in scrapie-infected mouse neuroblastoma cell cultures. Because the PrP region from 119 to 136 is very conserved in most species, this peptide may have inhibitory effects on PrP conversion in a wide variety of TSE diseases.

Characterization of multidrug transporter-mediated efflux of avermectins in human and mouse neuroblastoma cell lines

2015 ◽  
Vol 235 (3) ◽  
pp. 189-198 ◽  
Author(s):  
Abigail M. Dalzell ◽  
Pratibha Mistry ◽  
Jayne Wright ◽  
Faith. M. Williams ◽  
Colin. D.A. Brown
Keyword(s):  
Cell Lines ◽  
Neuroblastoma Cell ◽  
Multidrug Transporter ◽  
Mouse Neuroblastoma Cell ◽  
Mouse Neuroblastoma ◽  
Neuroblastoma Cell Lines ◽  
Human And Mouse

Clusterin Overexpression is Responsible for the Anti-apoptosis Effect in a Mouse Neuroblastoma Cell Line, B103

2003 ◽  
Vol 58 (1-2) ◽  
pp. 148-152 ◽  
Author(s):  
Kwan-Hee You ◽  
Young-Mi Ji ◽  
O-Yu Kwon
Keyword(s):  
Cell Line ◽  
Functional Role ◽  
Neuroblastoma Cell ◽  
Neuroblastoma Cell Line ◽  
Apoptotic Index ◽  
Mouse Neuroblastoma Cell ◽  
Mouse Neuroblastoma ◽  
Apoptosis Inhibition ◽  
Clusterin Expression

The functional role of clusterin in apoptosis was examined using flow cytometry. Clusterin cDNA was transfected into the mouse neuroblastoma cell line, B103, in order to determine if clusterin overexpression inhibits apoptosis. The increased clusterin expression level in the B103 cells tended to suppress the apoptotic index. This suggests an association of clusterin gene expression with apoptosis inhibition. These results support the conclusion that clusterin expression in B103 cells has an antiapoptotic influence

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