Escherichia coli biosensors for environmental, food industry and biological warfare agent detection

2013 ◽  
Author(s):  
R. C. S. B. Allil ◽  
M. M. Werneck ◽  
J. L. da Silva-Neto ◽  
M. A. L. Miguel ◽  
D. M. C. Rodrigues ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Food Industry ◽  
Biological Warfare ◽  
Biological Warfare Agent

scholarly journals Glanders: re-emergence of an ancient zoonosis

2020 ◽  
Vol 41 (1) ◽  
pp. 41
Author(s):  
Patricia Ellis
Keyword(s):  
Middle East ◽  
Human Health ◽  
Exponential Growth ◽  
First World War ◽  
Biological Warfare ◽  
World War ◽  
Biological Warfare Agent ◽  
The First World War ◽  
Global Spread ◽  
First World

Glanders, although known to be endemic in certain regions/countries of the Old and New Worlds for centuries, had been largely overlooked as a threat to equine and human health until the disease re-emerged in the Middle East in 2004. The exponential growth in international horse movements, both legal and illegal, mainly for performance purposes, has enhanced the risk of global spread of glanders in the Middle East and elsewhere. Ever since the First World War, the glanders bacillus has been recognised as a potential biological warfare agent.

scholarly journals Optimization of Electrolysis Parameters for Green Sanitation Chemicals Production Using Response Surface Methodology

Processes ◽  
2020 ◽  
Vol 8 (7) ◽  
pp. 792
Author(s):  
Nurul Izzah Khalid ◽  
Nurul Shaqirah Sulaiman ◽  
Norashikin Ab Aziz ◽  
Farah Saleena Taip ◽  
Shafreeza Sobri ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Food Industry ◽  
Chemical Properties ◽  
Bactericidal Effect ◽  
Electrolyzed Water ◽  
Optimum Parameters ◽  
Physico Chemical ◽  
Different Types ◽  
Different Temperatures ◽  
Optimized Conditions

Electrolyzed water (EW) shows great potential as a green and economical sanitation solution for the food industry. However, only limited studies have investigated the optimum electrolysis parameters and the bactericidal effect of acidic electrolyzed water (AcEW) and alkaline electrolyzed water (AlEW). Here, the Box–Behnken experimental design was used to identify the optimum parameters. The tests were conducted with different types of electrodes, electrical voltages, electrolysis times, and NaCl concentrations. There were no obvious differences observed in the physico-chemical properties of EW when different electrodes were used. However, stainless steel was chosen as it meets most of the selection criteria. The best-optimized conditions for AcEW were at 11.39 V, 0.65 wt.% NaCl, and 7.23 min, while the best-optimized conditions for AlEW were at 10.32 V, 0.6 wt.% NaCl, and 7.49 min. The performance of the optimum EW (AcEW and AlEW) compared with commercial cleaning detergents for the food industry was then evaluated. The bactericidal activity of AcEW and AlEW was examined against Escherichia coli ATCC 10536 at different temperatures (30 °C and 50 °C) for 30 s. The results show that both AcEW and AlEW have the ability to reduce the Escherichia coli to non-detectable levels (less than 2 log CFU/mL).

Standoff lidar simulation for biological warfare agent detection, tracking, and classification

2010 ◽  
Author(s):  
Erika Jönsson ◽  
Ove Steinvall ◽  
Ove Gustafsson ◽  
Fredrik Kullander ◽  
Per Jonsson
Keyword(s):  
Biological Warfare ◽  
Biological Warfare Agent

scholarly journals Cloning and expression of the acyl thioesterase 2 (ALT2) gene in Escherichia coli for producing short- and medium-chain 2-methylketones

2015 ◽  
Vol 18 (2) ◽  
pp. 87-98
Author(s):  
Vy Le Uyen Khuat ◽  
Thao Phuong Do ◽  
Thuong Thi Hong Nguyen
Keyword(s):  
Escherichia Coli ◽  
Food Industry ◽  
Biosynthetic Pathway ◽  
Biodiesel Production ◽  
Cloning And Expression ◽  
Melting Points ◽  
E Coli ◽  
Flame Ionization ◽  
Fatty Acid Biosynthetic Pathway ◽  
Natural Insecticides

2-Methylketones are organic compounds that are more widely used in the food industry and cosmetics. Some of methylketones found in plants act as pheromones and natural insecticides. Recently, methylketones have been recognized as strong candidates for the production of renewable energy as they possess not only favourable cetane numbers but also lower hydrophilicity and melting points than fatty acids which have been used in biodiesel production. In addition, short chain methylketones have much lower melting points than long chain methylketones. In this study, we cloned and expressed in Escherichia coli C41(DE3) cells a gene for acyl thioesterase 2 (ALT2) isolated from Arabidopsis thaliana. The ALT2 enzyme could hydrolyze 3-ketoacyl-ACP (also called β-ketoacyl-ACP) intermediates in the fatty acid biosynthetic pathway into the corresponding 3-ketoacid. The resulting 3- ketoacids are unstable compounds that will be decarboxylated to produce n-1 methylketones. Methylketones released in the growth medium of E. coli expressing ALT2 were extracted by hexane and analyzed by gas chromatography with the flame ionization detector (GC-FID). The results showed that the E. coli C41(DE3) cells expressing ALT2 recombinantly produced 2-nonanone (9C), 2- undecanone (11C) and 2-tridecanone (13C) in the spent medium when were induced with 0.25 mM IPTG at 37 oC for 3.5 hours.

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