A preliminary investigation on the interaction between sol-gel immobilized glucose oxidase and freely diffusing glucose by means of two-photon microscopy

2013 ◽  
Author(s):  
I. Delfino ◽  
M. Portaccio ◽  
M. De Rosa ◽  
M. Lepore
Keyword(s):  
Glucose Oxidase ◽  
Preliminary Investigation ◽  
Sol Gel ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Immobilized Glucose Oxidase

scholarly journals Glucose Sensing by Time-Resolved Fluorescence of Sol-Gel Immobilized Glucose Oxidase

Sensors ◽  
2011 ◽  
Vol 11 (4) ◽  
pp. 3483-3497 ◽  
Author(s):  
Rosario Esposito ◽  
Bartolomeo Della Ventura ◽  
Sergio De Nicola ◽  
Carlo Altucci ◽  
Raffaele Velotta ◽  
...  
Keyword(s):  
Glucose Oxidase ◽  
Sol Gel ◽  
Glucose Sensing ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Immobilized Glucose Oxidase

Chondrocyte Repopulation of Allograft Cartilage: A Preliminary Investigation and Strategy for Developing Cartilage Matrices for Reconstruction

2002 ◽  
Vol 127 (4) ◽  
pp. 265-270 ◽  
Author(s):  
Timothy F. Kelley ◽  
Frank M. Sutton ◽  
Vincent P. Wallace ◽  
Brian J. F. Wong
Keyword(s):  
Experimental Study ◽  
Medical Center ◽  
Academic Medical Center ◽  
Preliminary Investigation ◽  
Chondrocyte Viability ◽  
Freeze Thaw ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Academic Medical ◽  
Cultured Chondrocytes

OBJECTIVE: To evaluate the ability of devitalized cartilage to sustain repopulation by allogenic chondrocytes and to use 2-photon microscopy to assess cultured chondrocyte viability. STUDY DESIGN AND SETTING: At an academic medical center, we conducted experimental analysis of lagomorph nasal septa devitalized by subjecting them to either 1) chemical dehydration in ethanol, 2) lyophilization (freeze dry), or 3) repeated freeze-thaw (12 cycles) in liquid nitrogen. The processed cartilages were seeded with chondrocytes isolated from the septa of either the same rabbit or another rabbit and cultured for 4 weeks. They were assessed with a variety of techniques. RESULTS: The seeded chondrocytes maintained viability on the devitalized cartilage throughout the study period. Two-photon microscopy was effective in assessing the viability of cultured chondrocytes. CONCLUSION: Isolated lagomorph chondrocytes can be maintained in allogenic cartilage grafts devitalized using conventional physicochemical techniques. SIGNIFICANCE: This is the first experimental study to demonstrate that cultured chondrocytes can be maintained on devitalized cartilage.

In vivo imaging of liver injury and regeneration by functional two-photon microscopy

2016 ◽  
Vol 54 (12) ◽  
pp. 1343-1404
Author(s):  
A Ghallab ◽  
R Reif ◽  
R Hassan ◽  
AS Seddek ◽  
JG Hengstler
Keyword(s):  
Liver Injury ◽  
In Vivo Imaging ◽  
Two Photon Microscopy ◽  
Two Photon

Inorganic-organic Hybrid Materials for Real 3-D Sub-νm Lithography

2003 ◽  
Vol 780 ◽  
Author(s):  
R. Houbertz ◽  
J. Schulz ◽  
L. Fröhlich ◽  
G. Domann ◽  
M. Popall ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Sol Gel ◽  
Laser Pulses ◽  
Femtosecond Laser Pulses ◽  
Hybrid Polymer ◽  
Organic Hybrid ◽  
Two Photon ◽  
Applied Technology ◽  
Scale Structures ◽  
Sol Gel Synthesis

AbstractReal 3-D sub-νm lithography was performed with two-photon polymerization (2PP) using inorganic-organic hybrid polymer (ORMOCER®) resins. The hybrid polymers were synthesized by hydrolysis/polycondensation reactions (modified sol-gel synthesis) which allows one to tailor their material properties towards the respective applications, i.e., dielectrics, optics or passivation. Due to their photosensitive organic functionalities, ORMOCER®s can be patterned by conventional photo-lithography as well as by femtosecond laser pulses at 780 nm. This results in polymerized (solid) structures where the non-polymerized parts can be removed by conventional developers.ORMOCER® structures as small as 200 nm or even below were generated by 2PP of the resins using femtosecond laser pulses. It is demonstrated that ORMOCER®s have the potential to be used in components or devices built up by nm-scale structures such as, e.g., photonic crystals. Aspects of the materials in conjunction to the applied technology are discussed.

scholarly journals Adaptive Optical Two-Photon Microscopy for Surface-Profiled Living Biological Specimens

ACS Omega ◽  
2020 ◽  
Author(s):  
Kazushi Yamaguchi ◽  
Kohei Otomo ◽  
Yuichi Kozawa ◽  
Motosuke Tsutsumi ◽  
Tomoko Inose ◽  
...  
Keyword(s):  
Two Photon Microscopy ◽  
Two Photon

scholarly journals Publisher Correction: Miniature two-photon microscopy for enlarged field-of-view, multi-plane and long-term brain imaging

2021 ◽  
Vol 18 (2) ◽  
pp. 220-220
Author(s):  
Weijian Zong ◽  
Runlong Wu ◽  
Shiyuan Chen ◽  
Junjie Wu ◽  
Hanbin Wang ◽  
...  
Keyword(s):  
Brain Imaging ◽  
Field Of View ◽  
Two Photon Microscopy ◽  
Two Photon
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