New approach to double-helix point spread function design for 3D super-resolution microscopy

2013 ◽  
Author(s):  
Ginni Grover ◽  
Rafael Piestun
Keyword(s):  
Point Spread Function ◽  
Double Helix ◽  
Super Resolution ◽  
New Approach ◽  
Point Spread ◽  
Function Design ◽  
Spread Function ◽  
Super Resolution Microscopy

scholarly journals Three-Dimensional Super-Resolution in Eukaryotic Cells Using the Double-Helix Point Spread Function

2017 ◽  
Vol 112 (7) ◽  
pp. 1444-1454 ◽  
Author(s):  
Alexander R. Carr ◽  
Aleks Ponjavic ◽  
Srinjan Basu ◽  
James McColl ◽  
Ana Mafalda Santos ◽  
...  
Keyword(s):  
Point Spread Function ◽  
Double Helix ◽  
Three Dimensional ◽  
Super Resolution ◽  
Eukaryotic Cells ◽  
Point Spread ◽  
Spread Function

scholarly journals Metasurface integrated with double-helix point spread function and metalens for three-dimensional imaging

Nanophotonics ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 451-458 ◽  
Author(s):  
Chunqi Jin ◽  
Jihua Zhang ◽  
Chunlei Guo
Keyword(s):  
Particle Tracking ◽  
Point Spread Function ◽  
Double Helix ◽  
Three Dimensional ◽  
Super Resolution ◽  
Single Particle Tracking ◽  
Point Sources ◽  
Phase Mask ◽  
Point Spread ◽  
Spread Function

AbstractMetasurfaces are two-dimensional arrangements of antennas that control the propagation of electromagnetic waves with a subwavelength thickness and resolution. Previously, metasurfaces have been mostly used to obtain the function of a single optical element. Here, we demonstrate a plasmonic metasurface that represents the combination of a phase mask generating a double-helix point spread function (DH-PSF) and a metalens for imaging. DH-PSF has been widely studied in three-dimensional (3D) super-resolution imaging, biomedical imaging, and particle tracking, but the current DH-PSFs are inefficient, bulky, and difficult to integrate. The multielement metasurface, which we label as DH-metalens, enables a DH-PSF with transfer efficiency up to 70.3% and an ultrahigh level of optical system integration, three orders of magnitude smaller than those realized by conventional phase elements. Moreover, the demonstrated DH-metalens can work in broadband visible wavelengths and in multiple incident polarization states. Finally, we demonstrate the application of the DH-metalens in 3D imaging of point sources. These results pave ways for realizing integrated DH-PSFs, which have applications in 3D super-resolution microscopy, single particle tracking/imaging, and machine vision.

scholarly journals Super-resolution photon-efficient imaging by nanometric double-helix point spread function localization of emitters (SPINDLE)

2012 ◽  
Vol 20 (24) ◽  
pp. 26681 ◽  
Author(s):  
Ginni Grover ◽  
Keith DeLuca ◽  
Sean Quirin ◽  
Jennifer DeLuca ◽  
Rafael Piestun
Keyword(s):  
Point Spread Function ◽  
Double Helix ◽  
Super Resolution ◽  
Point Spread ◽  
Spread Function

scholarly journals High-fidelity structured illumination microscopy by point-spread-function engineering

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Gang Wen ◽  
Simin Li ◽  
Linbo Wang ◽  
Xiaohu Chen ◽  
Zhenglong Sun ◽  
...  
Keyword(s):  
Point Spread Function ◽  
Super Resolution ◽  
Reconstruction Algorithm ◽  
High Fidelity ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Imaging Tool ◽  
Point Spread ◽  
Spread Function ◽  
Point Spread Function Engineering

AbstractStructured illumination microscopy (SIM) has become a widely used tool for insight into biomedical challenges due to its rapid, long-term, and super-resolution (SR) imaging. However, artifacts that often appear in SIM images have long brought into question its fidelity, and might cause misinterpretation of biological structures. We present HiFi-SIM, a high-fidelity SIM reconstruction algorithm, by engineering the effective point spread function (PSF) into an ideal form. HiFi-SIM can effectively reduce commonly seen artifacts without loss of fine structures and improve the axial sectioning for samples with strong background. In particular, HiFi-SIM is not sensitive to the commonly used PSF and reconstruction parameters; hence, it lowers the requirements for dedicated PSF calibration and complicated parameter adjustment, thus promoting SIM as a daily imaging tool.

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