In vivo flow cytometry visualizes the effects of tumor resection on metastasis by real-time monitoring of rare circulating cancer cells

2013 ◽  
Author(s):  
Dan Wei ◽  
Zhichao Fan ◽  
Xueding Wang ◽  
Xunbin Wei
Keyword(s):  
Flow Cytometry ◽  
Cancer Cells ◽  
Real Time ◽  
Tumor Resection ◽  
Real Time Monitoring ◽  
Circulating Cancer Cells ◽  
In Vivo Flow Cytometry

scholarly journals Noninvasive and real-time monitoring of Au nanoparticle promoted cancer metastasis using in vivo flow cytometry

2021 ◽  
Vol 12 (4) ◽  
pp. 1846
Author(s):  
Wen Pang ◽  
Shihui Ding ◽  
Liyun Lin ◽  
Chen Wang ◽  
Man Lei ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Real Time ◽  
Cancer Metastasis ◽  
Au Nanoparticle ◽  
Real Time Monitoring ◽  
In Vivo Flow Cytometry

scholarly journals Real-time monitoring of circulating tumor cell (CTC) release after nanodrug or tumor radiotherapy using in vivo flow cytometry

2017 ◽  
Vol 492 (3) ◽  
pp. 507-512 ◽  
Author(s):  
Nathan A. Koonce ◽  
Mazen A. Juratli ◽  
Chengzhong Cai ◽  
Mustafa Sarimollaoglu ◽  
Yulian A. Menyaev ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Tumor Cell ◽  
Real Time ◽  
Circulating Tumor Cell ◽  
Real Time Monitoring ◽  
In Vivo Flow Cytometry

Monitoring circulating cancer cells by multichannel in vivo flow cytometry

2012 ◽  
Author(s):  
Costas Pitsillides ◽  
Konstantinos Kapnisis ◽  
Marianna Prokopi ◽  
Christina Kousparou ◽  
Andreas Anayiotos
Keyword(s):  
Flow Cytometry ◽  
Cancer Cells ◽  
Circulating Cancer Cells ◽  
In Vivo Flow Cytometry

Real-time monitoring of circulating tumor cell release during tumor manipulation using in vivo photoacoustic and fluorescent flow cytometry

Head & Neck ◽  
2013 ◽  
Vol 36 (8) ◽  
pp. 1207-1215 ◽  
Author(s):  
Mazen A. Juratli ◽  
Mustafa Sarimollaoglu ◽  
Eric R. Siegel ◽  
Dmitry A. Nedosekin ◽  
Ekaterina I. Galanzha ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Tumor Cell ◽  
Real Time ◽  
Circulating Tumor Cell ◽  
Real Time Monitoring ◽  
Cell Release

STUDYING THE ROLE OF MACROPHAGES IN CIRCULATING PROSTATE CANCER CELLS BY IN VIVO FLOW CYTOMETRY

2012 ◽  
Vol 05 (04) ◽  
pp. 1250027 ◽  
Author(s):  
JIN GUO ◽  
ZHICHAO FAN ◽  
ZHENGQIN GU ◽  
XUNBIN WEI
Keyword(s):  
Prostate Cancer ◽  
Flow Cytometry ◽  
Cancer Cells ◽  
The Body ◽  
Prostate Cancer Cells ◽  
In Vivo Flow Cytometry ◽  
Kinetics Of ◽  
Deficient Group

Metastasis is a very complicated multi-step process and accounts for the low survival rate of the cancerous patients. To metastasize, the malignant cells must detach from the primary tumor and migrate to secondary sites in the body through either blood or lymph circulation. Macrophages appear to be directly involved in tumor progression and metastasis. However, the role of macrophages in affecting cancer metastasis has not been fully elucidated. Here, we have utilized an emerging technique, namely in vivo flow cytometry (IVFC) to study the depletion kinetics of circulating prostate cancer cells in mice and determine how depletion of macrophages by the liposome-encapsulated clodronate affects the depletion kinetics. Our results show different depletion kinetics of PC-3 cells between the macrophage-deficient group and the control group. The number of circulating tumor cells (CTCs) in the macrophage-deficient group decreases in a slower manner compared to the control mice group. The differences in depletion kinetics indicate that the absence of macrophages facilitates the stay of prostate cancer cells in circulation. In addition, our imaging data suggest that macrophages might be able to arrest, phagocytose and digest PC-3 cells. Therefore, phagocytosis may mainly contribute to the depletion kinetic differences. The developed methods elaborated here would be useful to study the relationship between macrophages and tumor metastasis in small animal cancer models.

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