scholarly journals Monitoring subunit rotation in single FRET-labeled FoF1-ATP synthase in an anti-Brownian electrokinetic trap

2013 ◽  
Author(s):  
Thomas Heitkamp ◽  
Hendrik Sielaff ◽  
Anja Korn ◽  
Marc Renz ◽  
Nawid Zarrabi ◽  
...  
Keyword(s):  
Atp Synthase ◽  
Subunit Rotation ◽  
Fof1 Atp Synthase

scholarly journals Spotlighting motors and controls of single FoF1-ATP synthase

2013 ◽  
Vol 41 (5) ◽  
pp. 1219-1226 ◽  
Author(s):  
Michael Börsch ◽  
Thomas M. Duncan
Keyword(s):  
Escherichia Coli ◽  
Atp Synthase ◽  
Single Molecule ◽  
Conformational Changes ◽  
Structural Information ◽  
E Coli ◽  
Single Molecule Fret ◽  
Membrane Enzyme ◽  
Subunit Rotation ◽  
Fof1 Atp Synthase

Subunit rotation is the mechanochemical intermediate for the catalytic activity of the membrane enzyme FoF1-ATP synthase. smFRET (single-molecule FRET) studies have provided insights into the step sizes of the F1 and Fo motors, internal transient elastic energy storage and controls of the motors. To develop and interpret smFRET experiments, atomic structural information is required. The recent F1 structure of the Escherichia coli enzyme with the ϵ-subunit in an inhibitory conformation initiated a study for real-time monitoring of the conformational changes of ϵ. The present mini-review summarizes smFRET rotation experiments and previews new smFRET data on the conformational changes of the CTD (C-terminal domain) of ϵ in the E. coli enzyme.

scholarly journals The Regulatory C-Terminal Domain of Subunit   of FoF1 ATP Synthase Is Dispensable for Growth and Survival of Escherichia coli

2011 ◽  
Vol 193 (8) ◽  
pp. 2046-2052 ◽  
Author(s):  
N. Taniguchi ◽  
T. Suzuki ◽  
M. Berney ◽  
M. Yoshida ◽  
G. M. Cook
Keyword(s):  
Escherichia Coli ◽  
Atp Synthase ◽  
Growth And Survival ◽  
Terminal Domain ◽  
Fof1 Atp Synthase

scholarly journals Single-molecule Imaging of ATP-driven Stepping Rotation of FoF1-ATP Synthase Reconstituted into Supported Membrane

2009 ◽  
Vol 96 (3) ◽  
pp. 29a
Author(s):  
Ryota Iino ◽  
Khek-Chian Tham ◽  
Kazuhito V. Tabata ◽  
Hiroshi Ueno ◽  
Hiroyuki Noji
Keyword(s):  
Atp Synthase ◽  
Single Molecule ◽  
Single Molecule Imaging ◽  
Supported Membrane ◽  
Fof1 Atp Synthase
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