Lensless imaging system to quantify cell proliferation

2013 ◽  
Author(s):  
S. Vinjimore Kesavan ◽  
C. P. Allier ◽  
F. Navarro ◽  
F. Mittler ◽  
B. Chalmond ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Imaging System ◽  
Lensless Imaging

Mutual-intensity lensless imaging system

1993 ◽  
Vol 18 (5) ◽  
pp. 394 ◽  
Author(s):  
Pang-chen Sun
Keyword(s):  
Imaging System ◽  
Lensless Imaging ◽  
Mutual Intensity

Evolution process from ghost diffraction to ghost imaging in a lensless imaging system

2011 ◽  
Vol 50 (32) ◽  
pp. 6098 ◽  
Author(s):  
Yanfeng Bai ◽  
Haiyan Gao ◽  
Taigang Liu ◽  
Teng Qiu ◽  
Haiqing Zhou
Keyword(s):  
Imaging System ◽  
Evolution Process ◽  
Ghost Imaging ◽  
Lensless Imaging

scholarly journals Super-Resolution Lensless Imaging of Cells Using Brownian Motion

2019 ◽  
Vol 9 (10) ◽  
pp. 2080
Author(s):  
Yuan Fang ◽  
Ningmei Yu ◽  
Yuquan Jiang
Keyword(s):  
Brownian Motion ◽  
Imaging System ◽  
Imaging Technique ◽  
Super Resolution ◽  
Image Sensor ◽  
Oxide Semiconductor ◽  
Objective Lens ◽  
Cell Detection ◽  
Lensless Imaging ◽  
Cell Image

The lensless imaging technique, which integrates a microscope into a complementary metal oxide semiconductor (CMOS) digital image sensor, has become increasingly important for the miniaturization of biological microscope and cell detection equipment. However, limited by the pixel size of the CMOS image sensor (CIS), the resolution of a cell image without optical amplification is low. This is also a key defect with the lensless imaging technique, which has been studied by a many scholars. In this manuscript, we propose a method to improve the resolution of the cell images using the Brownian motion of living cells in liquid. A two-step algorithm of motion estimation for image registration is proposed. Then, the raw holographic images are reconstructed using normalized convolution super-resolution algorithm. The result shows that the effect of the collected cell image under the lensless imaging system is close to the effect of a 10× objective lens.

scholarly journals Testing Cellular Proliferation Responses to Oxidative, Glucocorticoid and Metabolic Challenges in the Baboon

2020 ◽  
Vol 4 (Supplement_1) ◽  
pp. 890-890
Author(s):  
Daniel Adekunbi ◽  
Cun Li ◽  
Peter Nathanielsz ◽  
Adam Salmon
Keyword(s):  
Oxidative Stress ◽  
Cell Proliferation ◽  
Cellular Response ◽  
Cellular Proliferation ◽  
Imaging System ◽  
Inhibitory Effect ◽  
Model Systems ◽  
Average Life Span ◽  
Young Males ◽  
Low Glucose

Abstract Aging is associated with progressive loss of cellular homeostasis which results from intrinsic and extrinsic challenges. Testing how such challenges relate to the aging process is often limited by the available model systems. We use primary cells (fibroblasts) isolated from baboons as a non-human primate cellular model to address how stressful challenges affect resilience. Using a real-time live-cell imaging system, we characterized a protocol for testing the effects of pro-oxidant compounds (e.g hydrogen peroxide (H2O2), paraquat and thapsigargin), dexamethasone and low glucose environment on cellular proliferation in fibroblasts derived from baboons across the life-course. The inhibitory effect of H2O2 (50 and 100µM), an oxidative stress, on cell proliferation was dose-dependent, with a higher impact in old males (age 14.52-14.80 years; average life span 21 years) compared to young males (6.35-6.39 years). Exposure to a different oxidative stress, paraquat (100 and 200µM) tended to reduce cell proliferation rate with age in males but not females. Inhibitory effects of thapsigargin, an endoplasmic reticulum stress inducer, on cell proliferation were dependent on challenge duration (2 vs 24h), concentration (0.1 and 1µM) and donor age, with greater resilience in young males than young females (4.33-6.70 years). Dexamethasone (100 and 500µM), a glucocorticoid, reduced cell proliferation dose-dependently, with older males exhibiting more resilience than females. In response to low glucose (1mM), cell proliferation reduced with age. Donor’s chronological age and sex are important variables in cellular response to challenge compounds faced during aging, which will guide our on-going studies on the cellular transcriptome and proteome.

Elliptocyte detection technology based on super-resolution algorithms for a lensless imaging system

2020 ◽  
Vol 32 (2) ◽  
pp. 025701
Author(s):  
Jianwei Li ◽  
Li Dai ◽  
Ningmei Yu ◽  
Zhengpeng Li ◽  
Shuaijun Li
Keyword(s):  
Imaging System ◽  
Super Resolution ◽  
Lensless Imaging ◽  
Detection Technology

Measurement of red blood cell size based on a lensless imaging system

2020 ◽  
Author(s):  
Jianwei Li ◽  
Li Dai ◽  
Ningmei Yu ◽  
Zhengpeng Li ◽  
Shuaijun Li
Keyword(s):  
Blood Cell ◽  
Cell Size ◽  
Red Blood Cell ◽  
Imaging System ◽  
Lensless Imaging

scholarly journals Interleukin (IL)-22 from IL-20 Subfamily of Cytokines Induces Colonic Epithelial Cell Proliferation Predominantly through ERK1/2 Pathway

2019 ◽  
Vol 20 (14) ◽  
pp. 3468 ◽  
Author(s):  
Md. Moniruzzaman ◽  
Ran Wang ◽  
Varinder Jeet ◽  
Michael A. McGuckin ◽  
Sumaira Z. Hasnain
Keyword(s):  
Cell Proliferation ◽  
Epithelial Cell ◽  
Epithelial Cells ◽  
Imaging System ◽  
Intestinal Epithelial Cells ◽  
Human Colon ◽  
Epithelial Cell Line ◽  
Intestinal Epithelial ◽  
Human Colon Cancer ◽  
Transcriptional Changes

The interleukin (IL)-20 subfamily of cytokines consists of IL-19, IL-20, IL-22, IL-24, and IL-26, and the expression of IL-20, IL-22, and IL-24 is reported to be higher in the colon of patients with ulcerative colitis. Although the receptors for these cytokines are highly expressed in the colon epithelium, their effects on epithelial renewal are not clearly understood. This study evaluated the effects of IL-20, IL-22, and IL-24 in epithelial renewal using the LS174T human colon cancer epithelial cell line. LS174T cells were treated with IL-20, IL-22, and IL-24 (25, 50, and 100 ng/mL) and a live-cell imaging system was used to evaluate the effects on cell proliferation. Following treatment, the signaling pathways contributing to cell proliferation were investigated through Western blotting in LS174T cells and downstream transcriptional changes through qRT-PCR in LS174T cells, and RNA-Seq in primary murine intestinal epithelial cells. Our results demonstrated that only IL-22 promoted LS174T cell proliferation, mediated via extracellular-signal-regulated kinase (ERK)1/2-mediated downstream regulation of p90RSK, c-Jun, and transcriptional changes of TRIM15 and STOM. IL-22 also promoted expression of ERK1/2-independent genes such as DDR2, LCN2, and LRG1, which are known to be involved in cell proliferation and migration. This study suggests that IL-22 induces cell proliferation in highly proliferative cells such as intestinal epithelial cells.

The evaluation of lymph node cell proliferation response by liposomes loaded with major histocompatibility complex class II binding aquaporin 4 antigen peptide

2020 ◽  
Vol 85 (3) ◽  
pp. 537-544
Author(s):  
Yo Muraki ◽  
Yutaka Nishimoto ◽  
Midori Yamasaki ◽  
Shuuichi Miyakawa ◽  
Shuji Sato
Keyword(s):  
Cell Proliferation ◽  
Dendritic Cells ◽  
Lymph Node ◽  
Imaging System ◽  
Ex Vivo ◽  
Aquaporin 4 ◽  
Antigen Peptide ◽  
New Strategy ◽  
Proliferation Response

ABSTRACT Autoimmune responses to aquaporin 4 (AQP4) cause neuromyelitis optica (NMO); thus, specific immunotolerance to this self-antigen could represent a new NMO treatment. We generated the liposome-encapsulated AQP4 peptide 201-220 (p201-220) to induce immunotolerance. Liposomes were generated using phosphatidylserine and the polyglycidol species PG8MG. The in vivo tissue distribution of the liposomes was tested using an ex vivo imaging system. To confirm the antigen presentation capacity of PG8MG liposomes, dendritic cells were treated with PG8MG liposome-encapsulated AQP4 p201-220 (AQP4-PG8MG liposomes). Immunotolerance induction by AQP4-PG8MG liposomes was evaluated using the ex vivo cell proliferation of lymph node cells isolated from AQP4 p201-220-immunized AQP4-deficient mice. Fluorescent dye-labeled PG8MG liposomes were distributed to the lymph nodes. AQP4 p201-220 was presented on dendritic cells. AQP4-PG8MG liposomes were tended to suppress immune responses to AQP4 p201-220. Thus, the encapsulation of AQP4 peptides in PG8MG liposomes represents a new strategy for suppressing autoimmune responses to AQP4.

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