Aggregation and deformation of red blood cells as probed by a laser light scattering technique in a concentrated suspension: comparison between normal and pathological red blood cells

1994 ◽  
Author(s):  
Amir H. Gandjbakhche ◽  
Ali Othmane ◽  
Pierre Mills ◽  
Patrick Snabre ◽  
Jacques Dufaux
Keyword(s):  
Light Scattering ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Laser Light ◽  
Laser Light Scattering ◽  
Concentrated Suspension ◽  
Scattering Technique ◽  
Light Scattering Technique

scholarly journals Necrotic and apoptotic volume changes of red blood cells investigated by low-angle light scattering technique

2007 ◽  
Vol 21 (2) ◽  
pp. 105-120 ◽  
Author(s):  
Igor V. Mindukshev ◽  
Vladimir V. Krivoshlyk ◽  
Elena E. Ermolaeva ◽  
Irina A. Dobrylko ◽  
Evgeniy V. Senchenkov ◽  
...  
Keyword(s):  
Light Scattering ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Blood Platelets ◽  
Calcium Ionophore ◽  
Scattering Technique ◽  
Rbc Aggregation ◽  
Ammonia Medium ◽  
Very High ◽  
Light Scattering Technique

A low-angle light scattering technique, which has been applied previously to studies of blood platelets and Ehrlich ascite tumor cells, revealed differences in the dynamics of necrotic and apoptotic red blood cell death. Under hypotonic loading or in ammonia medium, red blood cells (RBC) swelled to a critical size (diameter approximately 13μm) prior to hemolysis (necrosis). Under acidic loading, hemolysis occurred with less pronounced swelling of cells (diameter approximately 10μm). Apoptosis induced by a calcium ionophore resulted in initial formation of echinocytes, followed by development of rounded red blood cells with uneven membrane, capable of agglomeration. In such a way, RBC aggregation can precede the final stages of the RBC apoptosis when small cellular fragments are generated. On the basis of erythrograms of the cells hemolysing in ammonia medium, the echinocytic (preapoptotic) and stomatocytic (prenecrotic) RBC were discerned due to the very high resistance of apoptotic RBC to osmotic (ammonia) loading.

Precipitation Kinetics of Hydroxyapatite Revealed by the Continuous-Angle Laser Light-Scattering Technique

2000 ◽  
Vol 104 (45) ◽  
pp. 10563-10568 ◽  
Author(s):  
Kazuo Onuma ◽  
Ayako Oyane ◽  
Kazunori Tsutsui ◽  
Katsuharu Tanaka ◽  
Gabin Treboux ◽  
...  
Keyword(s):  
Light Scattering ◽  
Laser Light ◽  
Laser Light Scattering ◽  
Precipitation Kinetics ◽  
Scattering Technique ◽  
Kinetics Of ◽  
Light Scattering Technique
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