Laser blood flowmetry in malignant tumors during photodynamic therapy: an experimental study

1994 ◽  
Author(s):  
Peter Sieg ◽  
Juergen Rosperich ◽  
Alex Walther ◽  
Swantje Pfleumer
Keyword(s):  
Experimental Study ◽  
Photodynamic Therapy ◽  
Malignant Tumors ◽  
Blood Flowmetry

scholarly journals Zinc-Phthalocyanine-Loaded Extracellular Vesicles Increase Efficacy and Selectivity of Photodynamic Therapy in Co-Culture and Preclinical Models of Colon Cancer

Pharmaceutics ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1547
Author(s):  
Pablo Lara ◽  
Ruben V. Huis in ‘t Veld ◽  
Carla Jorquera-Cordero ◽  
Alan B. Chan ◽  
Ferry Ossendorp ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Photodynamic Therapy ◽  
Cancer Cells ◽  
Extracellular Vesicles ◽  
Malignant Tumors ◽  
Intratumoral Injection ◽  
Zinc Phthalocyanine ◽  
Tumor Cell Death ◽  
Culture Models

Photodynamic therapy (PDT) is a promising and clinically approved method for the treatment of cancer. However, the efficacy of PDT is often limited by the poor selectivity and distribution of the photosensitizers (PS) toward the malignant tumors, resulting in prolonged periods of skin photosensitivity. In this work, we present a simple and straightforward strategy to increase the tumor distribution, selectivity, and efficacy of lipophilic PS zinc phthalocyanine (ZnPc) in colon cancer by their stabilization in purified, naturally secreted extracellular vesicles (EVs). The PS ZnPc was incorporated in EVs (EV-ZnPc) by a direct incubation strategy that did not affect size distribution or surface charge. By using co-culture models simulating a tumor microenvironment, we determined the preferential uptake of EV-ZnPc toward colon cancer cells when compared with macrophages and dendritic cells. We observed that PDT promoted total tumor cell death in normal and immune cells, but showed selectivity against cancer cells in co-culture models. In vivo assays showed that after a single intravenous or intratumoral injection, EV-ZnPc were able to target the tumor cells and strongly reduce tumor growth over 15 days. These data expose opportunities to enhance the potential and efficacy of PDT using simple non-synthetic strategies that might facilitate translation into clinical practice.

scholarly journals AN EXPERIMENTAL STUDY OF WHOLE BLADDER WALL PHOTODYNAMIC THERAPY USING A LIGHT-SCATTERING MEDIUM

1988 ◽  
Vol 79 (5) ◽  
pp. 807-813
Author(s):  
Tadao Uchibayashi ◽  
Haruo Hisazumi ◽  
Toshiyasu Amano ◽  
Norio Miyoshi
Keyword(s):  
Experimental Study ◽  
Photodynamic Therapy ◽  
Light Scattering ◽  
Bladder Wall ◽  
Scattering Medium

Study of Photoinduced Antitumor Activity of Phthalocyanin-Based Nanostructures as Pro-Photosensitizers in Photodynamic Therapy of Malignant Tumors In Vivo

2014 ◽  
Vol 157 (6) ◽  
pp. 798-803 ◽  
Author(s):  
A. A. Pankratov ◽  
T. N. Andreeva ◽  
R. I. Yakubovskaya ◽  
B. Ya. Kogan ◽  
A. V. Butenin ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Antitumor Activity ◽  
Malignant Tumors

Effect of Axial Length on Laser Spot Size During Photodynamic Therapy: An Experimental Study in Monkeys

2006 ◽  
Vol 141 (1) ◽  
pp. 214-215 ◽  
Author(s):  
Mineo Kondo ◽  
Yasuki Ito ◽  
Kentaro Miyata ◽  
Nagako Kondo ◽  
Kohei Ishikawa ◽  
...  
Keyword(s):  
Experimental Study ◽  
Photodynamic Therapy ◽  
Axial Length ◽  
Spot Size ◽  
Laser Spot ◽  
Laser Spot Size

Efficacy of 5-aminolevulinic acid based photodynamic therapy in pituitary adenomas—experimental study on rat and human cell cultures

2016 ◽  
Vol 14 ◽  
pp. 77-83 ◽  
Author(s):  
Lisa Margarete Neumann ◽  
Kerim Beseoglu ◽  
Philipp Joerg Slotty ◽  
Brigitte Senger ◽  
Marcel Alexander Kamp ◽  
...  
Keyword(s):  
Experimental Study ◽  
Photodynamic Therapy ◽  
Cell Cultures ◽  
Human Cell ◽  
Pituitary Adenomas ◽  
Aminolevulinic Acid ◽  
Human Cell Cultures

scholarly journals Mechanism of Differential Susceptibility of Two (Canine Lung Adenocarcinoma) Cell Lines to 5-Aminolevulinic Acid-Mediated Photodynamic Therapy

Cancers ◽  
2021 ◽  
Vol 13 (16) ◽  
pp. 4174
Author(s):  
Tomohiro Osaki ◽  
Narumi Kunisue ◽  
Urara Ota ◽  
Hideo Imazato ◽  
Takuya Ishii ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Lung Adenocarcinoma ◽  
Cell Lines ◽  
Aminolevulinic Acid ◽  
Malignant Tumors ◽  
Protoporphyrin Ix ◽  
Invasive Treatment ◽  
No Donor ◽  
Adenocarcinoma Cell ◽  
Significant Difference

Photodynamic therapy (PDT) is a clinically approved, minimally invasive treatment for malignant tumors. Protoporphyrin IX (PpIX), derived from 5-aminolevulinic acid (5-ALA) as the prodrug, is one of the photosensitizers used in PDT. Recently, we reported a significant difference in response to 5-ALA-mediated PDT treatment in two canine primary lung adenocarcinoma cell lines (sensitive to PDT: HDC cells, resistant to PDT: LuBi cells). This study aimed to examine the difference in cytotoxicity of 5-ALA-mediated PDT in these cells. Although intracellular PpIX levels before irradiation were similar between HDC and LuBi cells, the percentage of ROS-positive cells and apoptotic cells in LuBi cells treated with 5-ALA-mediated PDT was significantly lower than that in HDC cells treated with 5-ALA-mediated PDT. A high dosage of the NO donor, DETA NONOate, significantly increased the cytotoxicity of 5-ALA-mediated PDT against LuBi cells. These results suggest that the sensitivity of 5-ALA-mediated PDT might be correlated with NO.

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