Photodynamic therapy (PDT) of endometrium primary cultures serving as an in-vitro model for endometriosis

1994 ◽  
Author(s):  
Wiebke Herter ◽  
Volker Viereck ◽  
J. Keckstein ◽  
Rudolf W. Steiner ◽  
Angelika C. Rueck
Keyword(s):  
Photodynamic Therapy ◽  
In Vitro Model ◽  
Primary Cultures ◽  
Vitro Model

scholarly journals An in vitro feasibility investigation considering primary human melanocytes for spray- grafting of freshly isolated autologous skin cells for burn treatment and a clinical case report

2019 ◽  
pp. 1-8
Author(s):  
Jörg C. Gerlach ◽  
C. Johnen ◽  
B. Hartmann ◽  
J. Plettig ◽  
K. Bräutigam ◽  
...  
Keyword(s):  
Cell Morphology ◽  
Skin Color ◽  
In Vitro Model ◽  
Primary Cultures ◽  
Cell Suspensions ◽  
Epidermal Keratinocytes ◽  
Cell Counts ◽  
Skin Coloration ◽  
Vitro Model

A skin cell-spray grafting technique that enables the on-site application of freshly isolated autologous single cell suspensions was already applied in many cases on caucasian patients with low skin coloration. Our project hypothesis is that these suspensions contain keratinocytes and vital melanocytes, that are of particular interest for the treatment of patients of darker skin color. To test this, we applied an in vitro model, wherein the feasibility of i) isolating and ii) spraying of freshly isolated autologous melanocyte-keratinocyte cell suspensions was investigated. Primary human epidermal keratinocytes (HEKs) and melanocytes (MCs) were isolated from skin biopsies (n=8). Biochemical parameter, cell counts, cell morphology, growth behavior and immunofluorescence results were compared in two groups using MC cultures and co-cultures of MCs with HEKs. Case information on using the method clinically with one patient is included. The sprayed mixed cell suspensions proliferated in all groups without measurable loss of viability, and MCs exhibited a regular cell morphology in monoculture up to passage 4°. The sprayed MCs and HEKs demonstrated in vitro glucose and lactate metabolism that was comparable to the pipetted controls. In co-culture, well distributed CK14+ HEKs and NKI/beteb+ MCs could be demonstrated, which interacted in the in vitro model. The ratio of HEKs : MCs in our primary cultures were microscopically counted (n=8 each) as mean +/- SD 1,211,000 (+/- 574,343) HEK : 99,625 (+/- 59,025) MC; i.e., a ratio of approx. 12 : 1. Using the isolation method clinically for a patient with dark skin coloration after suffering severe second-degree burns shows a satisfying re-pigmentation of the resulting wound post healing. Freshly isolated spray-on melanocyte/keratinocyte suspensions provide for a considerable amount of viable HEKs and MCs. Using MCs in spray-grafting suspensions could represent a promising approach for treating severe partial-thickness burns and innovative therapy developments that also aim to address cosmetic aspects.

scholarly journals Atlantic salmon cardiac primary cultures: An in vitro model to study viral host pathogen interactions and pathogenesis

PLoS ONE ◽  
2017 ◽  
Vol 12 (7) ◽  
pp. e0181058 ◽  
Author(s):  
Patricia A. Noguera ◽  
Bianka Grunow ◽  
Matthias Klinger ◽  
Katherine Lester ◽  
Bertrand Collet ◽  
...  
Keyword(s):  
Atlantic Salmon ◽  
In Vitro Model ◽  
Primary Cultures ◽  
Host Pathogen Interactions ◽  
Host Pathogen ◽  
Vitro Model

scholarly journals Peripheral neural cell sensitivity to mTHPC-mediated photodynamic therapy in a 3D in vitro model

2009 ◽  
Vol 101 (4) ◽  
pp. 658-665 ◽  
Author(s):  
K E Wright ◽  
E Liniker ◽  
M Loizidou ◽  
C Moore ◽  
A J MacRobert ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
In Vitro Model ◽  
Neural Cell ◽  
Cell Sensitivity ◽  
3D In Vitro Model ◽  
Vitro Model

Photodynamic therapy of endometriosis with HpD (Photosan III) in a new in vitro model

1994 ◽  
Author(s):  
Volker Viereck ◽  
Wiebke Werter ◽  
Angelika C. Rueck ◽  
Rudolf W. Steiner ◽  
J. Keckstein
Keyword(s):  
Photodynamic Therapy ◽  
In Vitro Model ◽  
Vitro Model

Visualizing photodynamic therapy response with time-lapse OCT in an in vitro model of metastatic ovarian cancer

2010 ◽  
Author(s):  
Conor L. Evans ◽  
Imran Rizvi ◽  
Jonathan Celli ◽  
Adnan Abu-Yousif ◽  
Johannes de Boer ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Photodynamic Therapy ◽  
In Vitro Model ◽  
Therapy Response ◽  
Time Lapse ◽  
Vitro Model

scholarly journals Ovine fetuses from slaughterhouses: A useful source for neural cell primary cultures

2021 ◽  
Author(s):  
Vittorio Farina ◽  
Sergio Domenico Gadau ◽  
Gianluca Lepore ◽  
Marcella Carcupino ◽  
Marco Zedda
Keyword(s):  
In Vitro Model ◽  
Primary Cultures ◽  
Medical Procedures ◽  
Cell Type ◽  
Experimental Animals ◽  
Vitro Model ◽  
Set Up ◽  
Very High ◽  
Β Tubulin

A lot of evidence demonstrates that sheep could represent an experimental model to set up medical procedures in view of their application on humans. Sheep are chosen as models for human biomechanical studies because their skeleton has some similarities to humans. The aim of this work was to set up sheep primary cultures from ovine fetuses at different ages, from pregnant uteri retrieved at local abattoirs. Cell characterization showed that one cell population was immunopositive to GFAP and identifiable as astrocytes, whereas a second cell type was III β-tubulin-positive, and hence classified as neurons. At 60-day old fetus is suitable to obtain neurons, whereas in a 90-day old fetus the cell culture is predominantly characterized by glial cells. The procedure here proposed is inexpensive, in fact, collecting fetuses during sheep slaughtering is a cost-saving option, unlike common experimental animals such as mice, rats, rabbits, that require very high economical efforts. Finally, our protocol fully eliminates the need of animal killing, being living animals replaced by a validated in vitro model in agreement with the 3Rs statement.

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