Photodynamic tumor therapy and on-line fluorescence spectroscopy after aminolevulinic acid administration using 633-nm light as therapeutic and fluorescence excitation radiation

1994 ◽  
Author(s):  
Karsten Koenig ◽  
Alwin Kienle ◽  
Wolf-Henning Boehncke ◽  
Roland Kaufmann ◽  
Angelika C. Rueck ◽  
...  
Keyword(s):  
Fluorescence Spectroscopy ◽  
Aminolevulinic Acid ◽  
Tumor Therapy ◽  
Fluorescence Excitation ◽  
Excitation Radiation ◽  
On Line ◽  
Acid Administration

CSIG-21. 5-ALA PDT AND TARGETING MEK/ERK SIGNALING ELICITS SYNERGISTIC ANTITUMOR EFFECTS IN DIFFUSE MIDLINE GLIOMA

2021 ◽  
Vol 23 (Supplement_6) ◽  
pp. vi37-vi37
Author(s):  
Gabrielle Price ◽  
Daniel Rivera ◽  
Alexandros Bouras ◽  
Constantinos Hadjipanayis
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Proliferation ◽  
Aminolevulinic Acid ◽  
Tumor Therapy ◽  
Unmet Need ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Antitumor Effects ◽  
Genetically Engineered Mouse Model

Abstract Diffuse midline gliomas (DMGs) are highly invasive, unresectable tumors in children. To date, there is no effective treatment for DMGs. Fractionated radiotherapy (RT), currently the standard of care, has provided limited disease control. Current obstacles to treatment include the blood brain barrier (BBB) that limits systemic drug delivery, tumor therapy resistance, and brainstem infiltration. Given the unmet need for more effective DMG treatments, photodynamic therapy (PDT), with the precursor photosensitizing agent 5-aminolevulinic acid (5-ALA), is an oncologic treatment that holds promise. 5-ALA PDT of tumors occurs by targeting tumor cells that accumulate the 5-ALA metabolite, protoporphyrin IX (PPIX), with 635 nm light to create deadly reactive oxygen species (ROS). We explore the synergism of 5-ALA PDT with the MEK inhibitor, trametinib, since the RAS/MEK signaling pathway regulates tumor cell proliferation and survival and has been shown to therapeutically enhance PDT in select tumor models. We demonstrated that sub-micromolar levels of 5-ALA PDT and nanomolar levels of trametinib successfully decrease cell proliferation and induce apoptosis in multiple DMG cell lines. Cell viability assays revealed that drug response differs based on the histone mutation (H3.1 or H3.3) of the line. Mechanisms of decreased cell survival involves the generation of reactive oxygen species that induces programmed cell death. Through the use of a DMG genetically engineered mouse model, we also found 5-ALA PDT to induce apoptosis in vivo. The synergistic effects of MEK inhibition and 5-ALA PDT in vitro and apoptotic effects of 5-ALA PDT in vivo, highlights the potential therapeutic efficacy of this treatment modality.

scholarly journals Interference with the Jaffé method for creatinine following 5-aminolevulinic acid administration

2010 ◽  
Vol 7 (4) ◽  
pp. 268-274 ◽  
Author(s):  
Harry Quon ◽  
Craig E. Grossman ◽  
Rebecca L. King ◽  
Mary Putt ◽  
Keri Donaldson ◽  
...  
Keyword(s):  
Aminolevulinic Acid ◽  
Acid Administration

A review on fluorescence spectroscopic analysis of water and wastewater

2021 ◽  
Author(s):  
Muhammad Farooq Saleem Khan ◽  
Mona Akbar ◽  
Jing Wu ◽  
Zhou Xu
Keyword(s):  
Water Quality ◽  
Organic Matter ◽  
Fluorescence Spectroscopy ◽  
Quality Assessment ◽  
Online Monitoring ◽  
Water Quality Assessment ◽  
Fluorescence Measurement ◽  
Fluorescence Excitation ◽  
Water And Wastewater ◽  
Fluorescence Peak

Abstract In recent years, the application of fluorescence spectroscopy has been widely recognized in water environment studies. The sensitiveness, simplicity, and efficiency of fluorescence spectroscopy are proved to be a promising tool for effective monitoring of water and wastewater. The fluorescence excitation-emission matrix (EEMs) and synchronous fluorescence spectra have been widely used analysis techniques of fluorescence measurement. The presence of organic matter in water and wastewater defines the degree and type of pollution in water. The application of fluorescence spectroscopy to characterize dissolved organic matter (DOM) has made the water quality assessment simple and easy. With the recent advances in this technology, components of DOM are identified by employing parallel factor analysis (PARAFAC), a mathematical trilinear data modeling with EEMs. The majority of wastewater studies indicated that the fluorescence peak of EX/EM at 275nm/340nm is referred to tryptophan region (Peak T1). However, some researchers identified another fluorescence peak in the region of EX/EM at 225-237nm/340-381nm, which described the tryptophan region and labeled it as Peak T2. Generally, peak T is a protein-like component in the water sample, where T1 and T2 signals were derived from the <0.20µm fraction of pollution. Therefore, a more advanced approach, such as an online fluorescence spectrofluorometer, can be used for the online monitoring of water. The results of various waters studied by fluorescence spectroscopy indicate that changes in peak T intensity could be used for real-time wastewater quality assessment and process control of wastewater treatment works. Finally, due to its effective use in water quality assessment, the fluorescence technique is proved to be a surrogate online monitoring tool and early warning equipment.

scholarly journals Prospects of fluorescence methods application for monitoring of cyanobacterial cultures in biotechnology

2020 ◽  
Vol 23 ◽  
pp. 02007
Author(s):  
Natalia Yu. Grigoryeva ◽  
Anna A. Liss
Keyword(s):  
Growth Rate ◽  
Fluorescence Spectroscopy ◽  
Food Production ◽  
Physiological State ◽  
Biotechnological Applications ◽  
Wastewater Remediation ◽  
Fluorescence Techniques ◽  
Fluorescence Methods ◽  
On Line ◽  
Photosynthetic Microorganisms

Cyanobacteria are photosynthetic microorganisms that possess a high potential for innovative applications in agriculture, food production, cosmeticals, wastewater remediation, biofuels, antioxidative enzymes production, etc. During the industrial cultivation of cyanobacteria several parameters as growth rate, physiological state and algological purity of the culture should be controled permanently. One of the methods that can provide on-line monitoring of cyanobacterial cultures and further process automatization is a fluorescence spectroscopy. In this work several fluorescence techniques are analysed and their possible adaptation for biotechnological applications is suggested.

On-line in situ monitoring of the soybean oil and ethanol transesterification reaction by fluorescence spectroscopy

Fuel ◽  
2015 ◽  
Vol 145 ◽  
pp. 109-115 ◽  
Author(s):  
T. Izida ◽  
L. Bussler ◽  
J.R. Silva ◽  
L.H.C. Andrade ◽  
E. Simionatto ◽  
...  
Keyword(s):  
Fluorescence Spectroscopy ◽  
Soybean Oil ◽  
Transesterification Reaction ◽  
In Situ Monitoring ◽  
On Line
Sign in / Sign up

Export Citation Format

Share Document