Imaging performance of the fiber optic image-bundle confocal microscope

1994 ◽  
Author(s):  
David J. Aziz ◽  
Arthur F. Gmitro
Keyword(s):  
Fiber Optic ◽  
Confocal Microscope ◽  
Imaging Performance ◽  
Optic Image

The influence of confocal microscope design on imaging performance

1993 ◽  
Vol 51 ◽  
pp. 144-145
Author(s):  
C J R Sheppard
Keyword(s):  
Image Quality ◽  
Fluorescence Imaging ◽  
Confocal Microscope ◽  
Industrial Applications ◽  
Three Dimensions ◽  
Design Parameters ◽  
Weak Signals ◽  
Size And Shape ◽  
Imaging Performance ◽  
Fundamental Limitation

The confocal microscope is now widely used in both biomedical and industrial applications for imaging, in three dimensions, objects with appreciable depth. There are now a range of different microscopes on the market, which have adopted a variety of different designs. The aim of this paper is to explore the effects on imaging performance of design parameters including the method of scanning, the type of detector, and the size and shape of the confocal aperture.It is becoming apparent that there is no such thing as an ideal confocal microscope: all systems have limitations and the best compromise depends on what the microscope is used for and how it is used. The most important compromise at present is between image quality and speed of scanning, which is particularly apparent when imaging with very weak signals. If great speed is not of importance, then the fundamental limitation for fluorescence imaging is the detection of sufficient numbers of photons before the fluorochrome bleaches.

Research on the scanning system of fiber optic confocal microscope

2001 ◽  
Author(s):  
Xinglong Wang ◽  
Zeying Chi ◽  
Wenjian Chen ◽  
Shuaia Wu
Keyword(s):  
Fiber Optic ◽  
Confocal Microscope ◽  
Scanning System

Design and fabrication of a fiber optic image inverter based on a new high numerical aperture fiber optic glasses system

2010 ◽  
Author(s):  
Jingsheng Pan ◽  
Jingwen Lv ◽  
Tao Zheng ◽  
Wenwei Liu ◽  
Wei Xu ◽  
...  
Keyword(s):  
Fiber Optic ◽  
Numerical Aperture ◽  
High Numerical Aperture ◽  
Optic Image

A three-dimensional shape measurement system based on fiber-optic image bundles

2014 ◽  
Author(s):  
Cheng Zhen ◽  
Huijie Zhao ◽  
Xiaoyue Liang ◽  
Hongzhi Jiang ◽  
Peijun Lv ◽  
...  
Keyword(s):  
Measurement System ◽  
Fiber Optic ◽  
Three Dimensional ◽  
Shape Measurement ◽  
Dimensional Shape ◽  
Three Dimensional Shape ◽  
Optic Image

Fiber-optic confocal microscope for biological imaging

1998 ◽  
Author(s):  
T. Collier ◽  
C. Smithpeter ◽  
B. Cowman ◽  
R. Drezek ◽  
M. Bescour ◽  
...  
Keyword(s):  
Fiber Optic ◽  
Confocal Microscope ◽  
Biological Imaging

Fiber optic image guide rods as ultrathin endoscopy

1991 ◽  
Author(s):  
Longin Kociszewski ◽  
Dariusz Pysz
Keyword(s):  
Fiber Optic ◽  
Image Guide ◽  
Optic Image

A Confocal Microscope with Spectrophotometry Detection

1999 ◽  
Vol 5 (S2) ◽  
pp. 460-461
Author(s):  
C. B. Calloway
Keyword(s):  
Focal Plane ◽  
Fiber Optic ◽  
Detection System ◽  
Confocal Microscope ◽  
Confocal Imaging ◽  
Spectral Information ◽  
Scanning System ◽  
Beam Path ◽  
Spectrophotometric Detection ◽  
Excitation Light

The Leica TCS SP Confocal Microscope combines spectrophotometric detection with confocal microcopy. The result is a multi-channel confocal imaging spectrophotometer that significantly increases the flexibility and efficiency of the detection system.In conventional point scanning confocal microscopes excitation light from laser(s) is delivered to the scan head via fiber optic, passed through a pinhole, reflected by a primary dichroic to the scanning system, and scanned onto the surface of the specimen. Light emitted from the specimen is descanned and passed to the detection system. The detection pinhole(s) are placed either between the primary dichroics and the detection system as in Leica confocal microscopes or closer to the end of the detection system. In the detection system emitted light is separated using a combination of dichroics, mirrors, and barrier filters before being passed to the photomultiplier detectors.The Leica TCS SP Spectral Confocal microscope has the same beam path as the filtered system up to and including the detection pinhole. The detection system including the secondary dichroics and the barrier filters is replaced by the spectrophotometer detection system (SP). The light emitted from the focal plane that passes through the detection pinhole has both intensity and spectral information. In the SP system this light is passed through a prism. The prism splits the emitted light into a spectrum from 400 to 750 nm. The spectrum is directed at a PMT for detection. The physical dimension of this spectrum is such that the entire spectrum can be imaged onto the window of the PMT.

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