New method for determination of ablation of dental hard tissues with the Er:YAG laser

1993 ◽  
Author(s):  
Harvey A. Wigdor ◽  
Joseph T. Walsh, Jr. ◽  
Joseph P. Cummings
Keyword(s):  
Er:Yag Laser ◽  
New Method ◽  
Dental Hard Tissues ◽  
Hard Tissues

scholarly journals Quantification of Bacterial Colonization in Dental Hard Tissues Using Optimized Molecular Biological Methods

2020 ◽  
Vol 11 ◽  
Author(s):  
Torsten Sterzenbach ◽  
Anne Pioch ◽  
Martin Dannemann ◽  
Christian Hannig ◽  
Marie-Theres Weber
Keyword(s):  
Biofilm Formation ◽  
Bacterial Colonization ◽  
Hard Tissue ◽  
Bacterial Dna ◽  
Root Canals ◽  
Dental Hard Tissue ◽  
Dental Hard Tissues ◽  
Hard Tissues ◽  
Biological Methods

Bacterial infections of root canals and the surrounding dental hard tissue are still a challenge due to biofilm formation as well as the complex root canal anatomy. However, current methods for analyzing biofilm formation, bacterial colonization of root canals and dental hard tissue [e.g., scanning electron microscopy, confocal laser scanning microscopy (CLSM) or determination of colony forming units (CFU)] are time-consuming and only offer a selective qualitative or semi-quantitative analysis. The aim of the present study is the establishment of optimized molecular biological methods for DNA-isolation and quantification of bacterial colonization via quantitative PCR (qPCR) from dental hard tissue. Root canals of human premolars were colonized with Enterococcus faecalis. For isolation of DNA, teeth were then grinded with a cryo mill. Since the hard tissues dentin and especially enamel belong to the hardest materials in the human organism, the isolation of bacterial DNA from root dentin is very challenging. Therefore, treatment steps for the isolation of DNA from grinded teeth were systematically analyzed to allow improved recovery of bacterial DNA from dental hard tissues. Starting with the disintegration of the peptidoglycan-layer of bacterial cells, different lysozyme solutions were tested for efficacy. Furthermore, incubation times and concentrations of chelating agents such as EDTA were optimized. These solutions are crucial for the disintegration of teeth and hence improve the accessibility of bacterial DNA. The final step was the determination of prior bacterial colonization of each root canal as determined by qPCR and comparing the results to alternative methods such as CFU. As a result of this study, optimized procedures for bacterial DNA-isolation from teeth were established, which result in an increased recovery rate of bacterial DNA. This method allows a non-selective and straightforward procedure to quantify bacterial colonization from dental hard tissue. It can be easily adapted for other study types such as microbiome studies and for comparable tissues like bones.

The Influence of Er:YAG Laser on the Dissolution Rate and Morphology of the Root Cement A scanning electron microscopic study

2018 ◽  
Vol 69 (6) ◽  
pp. 1574-1577
Author(s):  
Monica Monea ◽  
Daniela Esian ◽  
Reka Soos ◽  
Adrian Tohati ◽  
Alexandru Sitaru ◽  
...  
Keyword(s):  
Er:Yag Laser ◽  
Electron Microscopic ◽  
Morphological Alterations ◽  
Dental Tissues ◽  
Dental Hard Tissues ◽  
Hard Tissues ◽  
Root Planning ◽  
Scaling And Root Planning ◽  
Scanning Electron

Lasers can interact with dental tissues inducing changes in tooth characteristics, regarding the surface morphology and elemental composition. The alterations were observed in Calcium, Phosphorus, Sodium, Oxygen and Carbon elements, which influence the resistance and chemical properties of dental hard tissues. The thermal energy used by laser reduces water content of the tissues and as a consequence, the level of oxygen composition decreases after irradiation. Numerous studies had demonstrated its ability to ablate hard-tissues, without any detrimental thermal effects such as cracking or melting for the adjacent tissues. The aim of this article is to present the in vitro effects of Er:YAG laser in comparison with sonic, ultrasonic and manual instruments on the root cement during scaling and root planning, evaluated by scanning electron microscope. The study was conducted on extracted teeth, divided in four groups according to the method used for scaling and root planning. The specimens were analyzed by scanning electron microscopy and the morphological alterations of the cement were evaluated based on a scoring system. Data were statistically analyzed using Mann-Whitney test and the level of significance was set at p[0.05.We noted unfavorable results on the root cement after using Er:YAG laser as craters and cracks induced by heat. There was a greater amount of roughness on the root surface after Er:YAG was used for scaling and root planning compared to manual, sonic and ultrasonic methods. Despite favorable results obtained after the use of Er:YAG laser during periodontal treatment, further clinical studies are necessary in order to determine in which moment of the therapy these methods are most suitable.

Thermal effect of Er:YAG laser radiation on dental hard tissues

1993 ◽  
Author(s):  
Harvey A. Wigdor ◽  
Joseph T. Walsh, Jr. ◽  
Steven R. Visuri
Keyword(s):  
Laser Radiation ◽  
Thermal Effect ◽  
Er:Yag Laser ◽  
Dental Hard Tissues ◽  
Hard Tissues

Determination of ablation threshold of dental hard tissues irradiated with Er:YAG and Er,Cr:YSGG lasers

2013 ◽  
Vol 11 (5) ◽  
pp. 051701-51705
Author(s):  
Zhenlin Zhan Zhenlin Zhan ◽  
Xianzeng Zhang Xianzeng Zhang ◽  
Wenqing Guo Wenqing Guo ◽  
Shusen Xie Shusen Xie
Keyword(s):  
Ablation Threshold ◽  
Dental Hard Tissues ◽  
Hard Tissues

scholarly journals Tensile Bond Strength and Adaptability of One-bottle One-step Bonding Systems to Dental Hard Tissues Irradiated by Er:YAG Laser

2015 ◽  
Vol 26 (1) ◽  
pp. 17-27
Author(s):  
Naohiro IWATA ◽  
Keita YOKOTA ◽  
Youhei HIROTA ◽  
Kenzo YASUO ◽  
Kazushi YOSHIKAWA ◽  
...  
Keyword(s):  
Bond Strength ◽  
Er:Yag Laser ◽  
Tensile Bond Strength ◽  
Dental Hard Tissues ◽  
Hard Tissues ◽  
One Step ◽  
Bonding Systems

Study of the Chronologic Development and Eruption of Teeth in Elasmobranchs

1970 ◽  
Vol 49 (3) ◽  
pp. 556-560 ◽  
Author(s):  
Philip J. Boyne
Keyword(s):  
Phosphate Metabolism ◽  
Tetracycline Antibiotics ◽  
Dental Lamina ◽  
Dental Hard Tissues ◽  
Hard Tissues ◽  
Lemon Sharks ◽  
Calcium And Phosphate Metabolism

By the use of intravital labeling with tetracycline antibiotics, it has been possible to determine calcification patterns and the rate of exfoliation of elasmobranch teeth. The results indicate that young lemon sharks lose the most peripheral row of teeth in 14-day sequences, with a continuous replacement mechanism restoring the lost teeth from the deeper dental lamina. The technic used is considered to be applicable to the study of calcification mechanisms and calcium and phosphate metabolism in the elasmobranchs as well as to the determination of growth and exfoliation of dental hard tissues.

scholarly journals Reevaluation of the Enhancement Effect of Water-Spray in Ablation of Dental Hard Tissues with Use of the Er:YAG Laser

2010 ◽  
Vol 21 (1) ◽  
pp. 3-8 ◽  
Author(s):  
Toru TSUJIBAYASHI ◽  
Kazutoshi KAKIMOTO ◽  
Tetsuo FUKUOKA ◽  
Koichi TOYODA ◽  
Yutaka KOMASA
Keyword(s):  
Er:Yag Laser ◽  
Enhancement Effect ◽  
Water Spray ◽  
Effect Of Water ◽  
Dental Hard Tissues ◽  
Hard Tissues
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