Raman microspectroscopy and atomic force microscopy of chromosomal banding patterns

1993 ◽  
Author(s):  
Gerwin J. Puppels ◽  
Constant A. Putman ◽  
Bart G. de Grooth ◽  
Jan Greve
Keyword(s):  
Atomic Force Microscopy ◽  
Raman Microspectroscopy ◽  
Banding Patterns ◽  
Force Microscopy ◽  
Atomic Force ◽  
Chromosomal Banding

Karyotype Analysis of Buckwheat Using Atomic Force Microscopy

2011 ◽  
Vol 17 (4) ◽  
pp. 572-577 ◽  
Author(s):  
Suresh Neethirajan ◽  
Tamaki Hirose ◽  
Junichi Wakayama ◽  
Kazumi Tsukamoto ◽  
Hiroko Kanahara ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Karyotype Analysis ◽  
Three Dimensional ◽  
Evolutionary Trend ◽  
Common Buckwheat ◽  
Banding Patterns ◽  
Force Microscopy ◽  
Atomic Force ◽  
Root Tissues

AbstractKaryotype analysis and classification of buckwheat chromosomes were performed without chemical banding or staining using atomic force microscopy (AFM). Fagopyrum esculentum (common buckwheat) and Fagopyrum tartaricum (Tartarian buckwheat) chromosomes were isolated from root tissues using an enzymatic maceration technique and spread over a glass substrate. Air-dried chromosomes had a surface with ridges, and the height of common and tartary buckwheat were approximately 350 and 150 nm. Volumes of metaphase sets of buckwheat chromosomes were calculated using three-dimensional AFM measurements. Chromosomes were morphologically characterized by the size, volume, arm lengths, and ratios. The calculated volumes of the F. esculentum and F. tartaricum chromosomes were in the ranges of 1.08–2.09 μm3 and 0.49–0.78 μm3, respectively. The parameters such as the relative arm length, centromere position, and the chromosome volumes measured using AFM provide accurate karyomorphological classification by avoiding the subjective inconsistencies in banding patterns of conventional methods. The karyotype evolutionary trend indicates that F. esculentum is an ancient species compared to F. tartaricum. This is the first report of a cytological karyotype of buckwheat using AFM.

scholarly journals Characterization of Topical Film-Forming Systems Using Atomic Force Microscopy and Raman Microspectroscopy

2015 ◽  
Vol 12 (3) ◽  
pp. 751-757 ◽  
Author(s):  
Hazel Garvie-Cook ◽  
Kit Frederiksen ◽  
Karsten Petersson ◽  
Richard H. Guy ◽  
Sergey Gordeev
Keyword(s):  
Atomic Force Microscopy ◽  
Raman Microspectroscopy ◽  
Force Microscopy ◽  
Atomic Force ◽  
Film Forming
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