Confocal fluorescence microscopy of living cells

1993 ◽  
Author(s):  
Silvia M. Doglia ◽  
L. Bianchi ◽  
Roberto Colombo ◽  
N. Allam ◽  
Hamid Morjani ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Living Cells ◽  
Confocal Fluorescence Microscopy ◽  
Confocal Fluorescence

scholarly journals Interfacing 3D magnetic twisting cytometry with confocal fluorescence microscopy to image force responses in living cells

2017 ◽  
Vol 12 (7) ◽  
pp. 1437-1450 ◽  
Author(s):  
Yuejin Zhang ◽  
Fuxiang Wei ◽  
Yeh-Chuin Poh ◽  
Qiong Jia ◽  
Junjian Chen ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Image Force ◽  
Living Cells ◽  
Confocal Fluorescence Microscopy ◽  
Magnetic Twisting Cytometry ◽  
Confocal Fluorescence

Time-resolved 3D confocal fluorescence microscopy on living cells

2010 ◽  
Author(s):  
M. Schellenberg ◽  
E. Peev ◽  
M. Kloster ◽  
J. Napier ◽  
W. Neu
Keyword(s):  
Fluorescence Microscopy ◽  
Living Cells ◽  
Confocal Fluorescence Microscopy ◽  
Time Resolved ◽  
Confocal Fluorescence

A low affinity nanoparticle based fluorescent ratiometric probe for the determination of Zn(ii) concentrations in living cells

2018 ◽  
Vol 42 (18) ◽  
pp. 14986-14993
Author(s):  
Graham R. C. Hamilton ◽  
Simanpreet Kaur ◽  
Sukanta Kamila ◽  
Bridgeen Callan ◽  
John F. Callan
Keyword(s):  
Aqueous Solution ◽  
Fluorescence Microscopy ◽  
Fluorescent Probe ◽  
Living Cells ◽  
Confocal Fluorescence Microscopy ◽  
Confocal Fluorescence ◽  
Ratiometric Probe

A ratiometric polymeric fluorescent probe for Zn(ii) was developed capable of measuring Zn(ii) concentrations in aqueous solution between 0 and 5 mM and was also capable of discriminating between resting and high Zn(ii) levels in living cells using confocal fluorescence microscopy.

scholarly journals Analysis of Polyhydroxyalkanoates Granules in Haloferax mediterranei by Double-Fluorescence Staining with Nile Red and SYBR Green by Confocal Fluorescence Microscopy

Polymers ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1582
Author(s):  
Verónica Cánovas ◽  
Salvador Garcia-Chumillas ◽  
Fuensanta Monzó ◽  
Lorena Simó-Cabrera ◽  
Carmen Fernández-Ayuso ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Culture Media ◽  
Nile Red ◽  
Sybr Green ◽  
Confocal Fluorescence Microscopy ◽  
Fluorescence Staining ◽  
Haloferax Mediterranei ◽  
Optimized Protocol ◽  
Confocal Fluorescence ◽  
Pha Production

Haloferaxmediterranei is a haloarchaeon of high interest in biotechnology because it produces and mobilizes intracellular polyhydroxyalkanoate (PHA) granules during growth under stress conditions (limitation of phosphorous in the culture media), among other interesting metabolites (enzymes, carotenoids, etc.). The capability of PHA production by microbes can be monitored with the use of staining-based methods. However, the staining of haloarchaea cells is a challenging task; firstly, due to the high ionic strength of the medium, which is inappropriate for most of dyes, and secondly, due to the low permeability of the haloarchaea S-layer to macromolecules. In this work, Haloferax mediterranei is used as a halophilic archaeon model to describe an optimized protocol for the visualization and analysis of intracellular PHA granules in living cells. The method is based on double-fluorescence staining using Nile red and SYBR Green by confocal fluorescence microscopy. Thanks to this method, the capability of PHA production by new haloarchaea isolates could be easily monitored.

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