Synergism between photochemical and ionizing radiation effects in MCF-7 cells in vitro

1992 ◽  
Author(s):  
Gulshan Ara ◽  
Terence S. Herman ◽  
Archana Varshney ◽  
Timothy Korbut ◽  
Beverly A. Teicher
Keyword(s):  
Ionizing Radiation ◽  
Radiation Effects ◽  
Mcf 7

In vitro evaluation of ionizing radiation effects in bone tissue by FTIR spectroscopy

2015 ◽  
Author(s):  
Denise Maria Zezell ◽  
Marcelo Noronha Veloso ◽  
Derly Augusto Dias ◽  
Rodolfo Politano ◽  
Carolina Benetti
Keyword(s):  
Ionizing Radiation ◽  
Ftir Spectroscopy ◽  
Bone Tissue ◽  
Radiation Effects ◽  
In Vitro Evaluation

In vitro survival of MCF-7 breast cancer cells following combined treatment with ionizing radiation and mitoxantrone-mediated photodynamic therapy

2013 ◽  
Vol 10 (1) ◽  
pp. 72-78 ◽  
Author(s):  
Ameneh Sazgarnia ◽  
Ali Reza Montazerabadi ◽  
Mohammad Hossein Bahreyni-Toosi ◽  
Amirhossein Ahmadi ◽  
Amir Aledavood
Keyword(s):  
Breast Cancer ◽  
Photodynamic Therapy ◽  
Ionizing Radiation ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Combined Treatment ◽  
In Vitro Survival ◽  
Mcf 7

scholarly journals Flow cytometry protocol to evaluate ionizing radiation effects on P-glycoprotein activity

2008 ◽  
Vol 51 (spe) ◽  
pp. 109-120 ◽  
Author(s):  
Neyliane Gonçalves dos Santos ◽  
Ademir Amaral ◽  
Mariana Brayner Cavalcanti ◽  
Maria Amélia Batista Neves ◽  
Cíntia Gonsalves de Faria Machado
Keyword(s):  
Flow Cytometry ◽  
Ionizing Radiation ◽  
Radiation Effects ◽  
Human Peripheral Blood ◽  
Patient Specific ◽  
Glycoprotein P ◽  
Subject Analysis ◽  
P Glycoprotein ◽  
Different Doses

The aim of this work was to establish a protocol to evaluate ionizing radiation effects on P-glycoprotein (P-gp) activity. For this, human peripheral blood samples were irradiated in vitro with different doses and P-gp activity was analyzed for CD4 and CD8 T lymphocytes through rhodamine123-efflux assay by flow cytometry. By simultaneous employment of percentage and mean fluorescence index parameters, subject-by-subject analysis pointed out changes in P-gp activity for some individuals and irradiated samples. Based on this work, the proposed protocol was considered adequate for evaluating P-gp activity on cells after radioactive stress. Besides, this research suggests that P-gp activity could be an important factor to define patient-specific protocols in combined chemo-and radiotherapy, particularly when radiation exposure precedes chemical treatment.

Ionizing Radiation Effects In Vitro Study; Using The Rat Whole Embryo Culture Model

2021 ◽  
Vol 6 (13) ◽  
pp. 91-100
Author(s):  
Esra Balcioglu ◽  
Munevver Baran ◽  
Mehtap Nisari ◽  
Ozge Goktepe ◽  
Pinar Bilgici ◽  
...  
Keyword(s):  
Ionizing Radiation ◽  
Embryo Culture ◽  
Radiation Effects ◽  
Cell Number ◽  
Yolk Sac ◽  
Culture Period ◽  
Histological Evaluation ◽  
Ionizing Irradiation ◽  
Whole Embryo Culture

Background: Ionizing radiation poses a threat to the early embryo possibly leading to prenatal death, growth retardation, organ malformation, or mental retardation. It is important, assessment of any adverse effects of radiation upon the embryo. This study aimed to evaluate the outcomes of embryos irradiated with 1Gy doses in vitro and investigate hematopoiesis in the yolk sac of the irradiated embryos. Materials and methods: In the study, the experimental group of rats was be exposed to total body ionizing radiation on days 8.5th of gestation. All embryos in the control and radiation group cultured from gestation day 9.5 to 11.5 were alive at the end of the culture period. After 48 hours culture period, the embryos from each group were harvested and analyzed morphologically. Histological evaluation of the vWF+ cell number was performed in vivo. Results: The results showed that the embryonic growth and development during organogenesis decreased in the radiation exposed embryos when compared to control embryos. Additionally, the immunofluorescent examination showed that the vWF+ cell number reduced in the yolk sac of embryos exposed to ionizing radiation. Conclusion: Consequently, these findings support the conclusion that 1 Gy ionizing irradiation may increase prenatal death, intrauterine growth restriction on embryonic development when ionizing irradiation decreases the vWF+ cell number in the yolk sac compared to control embryos. This research related to radiation was the first study using the in vitro embryo culture technique; thus, future studies that will be performed by using different doses of radiation will contribute to the literature.

PLK1 Inhibition Radiosensitizes Breast Cancer Cells, but Shows Low Efficacy as Monotherapy or in Combination with other Cytotoxic Drugs

2019 ◽  
Vol 18 (9) ◽  
pp. 1252-1257 ◽  
Author(s):  
María Sol Brassesco ◽  
Julia Alejandra Pezuk ◽  
Karina Bezerra Salomão ◽  
Gabriela Molinari Roberto ◽  
Carlos Alberto Scrideli ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Ionizing Radiation ◽  
Drug Interactions ◽  
Cell Lines ◽  
Breast Cancer Cell Lines ◽  
Proteomic Profiling ◽  
In Vitro Effects ◽  
Near Future ◽  
Mcf 7

Background and Purpose: Over the last decade, the inhibition of PLK1 has proven potent antiproliferative activity in vitro. However, the effectiveness of most synthetic targeted drugs has not yet been translated into clinics. Herein, we investigated the in vitro effects of two second-generation PLK1 inhibitors BI 6727 and GSK461364 in breast cancer cell lines as monotherapy or in combination with other drugs or ionizing radiation. Material and Methods: Cell survival was analyzed through XTT®, clonogenicity and caspase-3 activation assays were also studied, and drug interactions analyzed through a nonlinear regression of a sigmoid doseresponse model. Sensibilization to radiation was assessed through enhancement ratio calculation. Results: Mild effects on the viability of both cell lines tested (MCF-7 and Hs578T) were observed irrespective of the used PLK1 inhibitor. Alternatively, abrogation of PLK1 significantly reduced clonogenicity while effectively sensitized cells to ionizing radiation. Drug interactions showed dissimilar results with antagonistic effects with any drug combination in MCF-7 and clear synergic interactions between both PLK1 inhibitors and cisplatin, temozolomide or doxorubicin in Hs578T, which is TP53 mutated. Conclusion: Targeting kinases involved in mitotic checkpoints are expected to prevent mitotic exit and enhance chemosensitization. Nonetheless, despite overexpressing PLK1, in our model, expressive results after its inhibition were only seen through clonogenic assays or when BI 6727 and GSK461364 were combined with ionizing radiation. Disparate responses of cell lines to drug combinations might denote a partial reflection of the substantial differences in the vast spectrum of genetic, biological and epigenetic burden observed in breast cancer. In the near future, individual genomic/proteomic profiling will allow its further classification and will consent the initiation of novel strategies for therapy. Even though the future impact of PLK1-tailored treatment still needs validation, much more pre-clinical and clinical research for this kinase are warranted.

Anticancer Potential of Calli Versus Seedling Extracts Derived from Rosmarinus officinalis and Coleus hybridus

2020 ◽  
Vol 21 (14) ◽  
pp. 1528-1538
Author(s):  
Sarah Albogami ◽  
Hadeer Darwish ◽  
Hala M. Abdelmigid ◽  
Saqer Alotaibi ◽  
Ahmed Nour El-Deen ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Transcriptional Profiling ◽  
Significant Inhibition ◽  
Rosmarinus Officinalis ◽  
Crude Extracts ◽  
Incidence And Mortality ◽  
Mcf 7

Background: In Saudi Arabia, the incidence and mortality rates of breast cancer are high. Although current treatments are effective, breast cancer cells develop resistance to these treatments. Numerous studies have demonstrated that active compounds in plant extracts, such as the phenolic compound Rosmarinic Acid (RA), exert anti-cancer effects. Objective: We investigated the anticancer properties of methanolic crude extracts of seedlings and calli of Rosmarinus officinalis and Coleus hybridus, two Lamiaceae species. Methods: MCF-7 human breast cancer cells were treated with methanolic crude extracts obtained from plant calli and seedlings generated in vitro, and cell proliferation was evaluated. Transcriptional profiling of the seedling and callus tissues was also conducted. Results: The mRNA expression levels of RA genes were higher in C. hybridus seedlings than in R. officinalis seedlings, as well as in C. hybridus calli than in R. officinalis calli, except for TAT and C4H. In addition, seedling and callus extracts of both R. officinalis and C. hybridus showed anti-proliferative effects against MCF-7 cells after 24 or 48 h of treatment. Discussion: At a low concentration of 10 μg/mL, C. hybridus calli and seedling extracts showed the most significant anti-proliferative effects after 24 and 48 h of exposure (p < 0.01); controls (doxorubicin) also showed significant inhibition, but lesser than that observed with C. hybridus (p < 0.05). Results with R. officinalis callus and seedling extracts did not significantly differ from those with untreated cells. Conclusion: Methanolic extracts of R. officinalis and C. hybridus are potentially valuable options for breast cancer treatment.

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