Near Real Time Confocal Microscopy of Cultured Amelanotic Cells: Sources of Signal, Contrast Agents and Limits of Contrast

1998 ◽  
Vol 3 (4) ◽  
pp. 429 ◽  
Author(s):  
Colin Smithpeter
Keyword(s):  
Confocal Microscopy ◽  
Contrast Agents ◽  
Real Time ◽  
Signal Contrast

scholarly journals Real‐time two‐photon confocal microscopy using a femtosecond, amplified Ti:sapphire system

1996 ◽  
Vol 181 (3) ◽  
pp. 253-259 ◽  
Author(s):  
G. J. BRAKENHOFF ◽  
J. SQUIER ◽  
T. NORRIS ◽  
A. C. BLITON ◽  
M. H. WADE ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Real Time ◽  
Two Photon

Real-time confocal microscopy imaging of corneal cytoarchitectural changes induced by different stresses

2021 ◽  
pp. 108706
Author(s):  
Guoliang Wang ◽  
Xiaoya An ◽  
Xiaoping Zhou ◽  
Mengyi Jin ◽  
Xuemei Wang ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Real Time ◽  
Microscopy Imaging ◽  
Confocal Microscopy Imaging

scholarly journals AIE-Driven Fluorescent Polysaccharide Polymersome as Enzyme-responsive FRET Nanoprobe to Study the Real-time Delivery Aspects in Live-Cells

2021 ◽  
Author(s):  
Nilesh Umakant Deshpande ◽  
Mishika Virmani ◽  
Manickam Jayakannan
Keyword(s):  
Energy Transfer ◽  
Confocal Microscopy ◽  
Real Time ◽  
Fluorescence Resonance Energy Transfer ◽  
Imaging Techniques ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Live Cells ◽  
Intracellular Enzyme ◽  
Bio Imaging

We report aggregation induced emission (AIE) driven polysaccharide polymersome as fluorescence resonance energy transfer (FRET) nanoprobes to study their intracellular enzyme-responsive delivery by real-time live-cell confocal microscopy bio-imaging techniques. AIE...

scholarly journals Targeting Contrast Agents With Peak Near-Infrared-II (NIR-II) Fluorescence Emission for Non-invasive Real-Time Direct Visualization of Thrombosis

2021 ◽  
Vol 8 ◽  
Author(s):  
Kenneth S. Hettie
Keyword(s):  
Contrast Agents ◽  
Real Time ◽  
Near Infrared ◽  
Fluorescence Emission ◽  
Therapeutic Window ◽  
Direct Visualization ◽  
Spatiotemporal Resolution ◽  
Accurate Identification ◽  
Non Invasive ◽  
Nirf Imaging

Thrombosis within the vasculature arises when pathological factors compromise normal hemostasis. On doing so, arterial thrombosis (AT) and venous thrombosis (VT) can lead to life-threatening cardio-cerebrovascular complications. Unfortunately, the therapeutic window following the onset of AT and VT is insufficient for effective treatment. As such, acute AT is the leading cause of heart attacks and constitutes ∼80% of stroke incidences, while acute VT can lead to fatal therapy complications. Early lesion detection, their accurate identification, and the subsequent appropriate treatment of thrombi can reduce the risk of thrombosis as well as its sequelae. As the success rate of therapy of fresh thrombi is higher than that of old thrombi, detection of the former and accurate identification of lesions as thrombi are of paramount importance. Magnetic resonance imaging, x-ray computed tomography (CT), and ultrasound (US) are the conventional non-invasive imaging modalities used for the detection and identification of AT and VT, but these modalities have the drawback of providing only image-delayed indirect visualization of only late stages of thrombi development. To overcome such limitations, near-infrared (NIR, ca. 700–1,700 nm) fluorescence (NIRF) imaging has been implemented due to its capability of providing non-invasive real-time direct visualization of biological structures and processes. Contrast agents designed for providing real-time direct or indirect visualization of thrombi using NIRF imaging primarily provide peak NIR-I fluorescence emission (ca. 700–1,000 nm), which affords limited tissue penetration depth and suboptimal spatiotemporal resolution. To facilitate the enhancement of the visualization of thrombosis via providing detection of smaller, fresh, and/or deep-seated thrombi in real time, the development of contrast agents with peak NIR-II fluorescence emission (ca. 1000–1,700 nm) has been recently underway. Currently, however, most contrast agents that provide peak NIR-II fluorescence emissions that are purportedly capable of providing direct visualization of thrombi or their resultant occlusions actually afford only the indirect visualization of such because they only provide for the (i) measuring of the surrounding vascular blood flow and/or (ii) simple tracing of the vasculature. These contrast agents do not target thrombi or occlusions. As such, this mini review summarizes the extremely limited number of targeting contrast agents with peak NIR-II fluorescence emission developed for non-invasive real-time direct visualization of thrombosis that have been recently reported.

scholarly journals A Real-Time Peak Extraction Algorithm for Dynamic Displacement Measurement Based on Spectral Confocal Microscopy

2021 ◽  
Vol 2112 (1) ◽  
pp. 012015
Author(s):  
Lijia Liu ◽  
Hua Ma ◽  
Zhendong Shi ◽  
Jinxi Bai ◽  
lin Zhang
Keyword(s):  
Confocal Microscopy ◽  
Real Time ◽  
Zero Point ◽  
Thickness Measurement ◽  
Displacement Measurement ◽  
Calculation Algorithm ◽  
Time Dynamic ◽  
Field Programmable ◽  
Important Position ◽  
Peak Value

Abstract Spectral confocal is widely used in various fields such as topography detection, roughness measurement, thickness measurement, for its advantages ability in displacement measurement. Developed from confocal microscopy, the spectral confocal microscopy greatly improves the efficiency of displacement measurement because there is no need for longitudinal scanning, In other words, we need to obtain the wavelength information corresponding to the peak point of the spectral signal, which is called peak abscissa but not the peak value. Therefore, Accurate and efficient peak abscissa calculation algorithm occupies an important position in displacement monitoring based on spectral confocal. However, the existing methods are too complex to apply to real-time dynamic online detection, for they usually focus on determining the peak value by means of curve fitting and peak extraction. In this paper, we proposed an efficient and accurate peak abscissa calculation method by shifting, difference, linear fitting, zero point and peak abscissa calculation (SDLZ). Compared with the Gaussian fitting method, the results demonstrate that the SDLZ can greatly improve efficiency, can be applied to field programmable gate array microcontrollers, with high measurement accuracy, providing key solutions for equipment needs.

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