scholarly journals Simultaneous fluorescence and positron emission tomography for in vivo imaging of small animals

2011 ◽  
Vol 16 (12) ◽  
pp. 120511 ◽  
Author(s):  
Bin Zhang
Keyword(s):  
Positron Emission Tomography ◽  
Emission Tomography ◽  
Small Animals ◽  
Positron Emission

scholarly journals Rapid Feasibility Studies of Tracers for Positron Emission Tomography: High-Resolution PET in Small Animals with Kinetic Analysis

1991 ◽  
Vol 11 (6) ◽  
pp. 926-931 ◽  
Author(s):  
M. Ingvar ◽  
L. Eriksson ◽  
G. A. Rogers ◽  
S. Stone-Elander ◽  
L. Widén
Keyword(s):  
Positron Emission Tomography ◽  
Emission Tomography ◽  
Whole Body ◽  
Small Animals ◽  
Pharmacological Properties ◽  
Time Activity ◽  
Pet Tracers ◽  
Arterial Blood ◽  
Positron Emission

The development of methods for production of a radiotracer for use in human studies with positron emission tomography (PET) is often a time-consuming process of optimizing radiolabelling yields and handling procedures. Sometimes the radiotracer is not the original drug, but rather a derivative with unknown in vivo pharmacological properties. We have developed a fast and simple method of testing putative new PET tracers in vivo in small animals. The procedure has been validated in rats with different PET tracers with known kinetic and pharmacological properties ([2-18F]2-fluoro-2-deoxy-d-glucose, [ N-methyl-11C]Ro 15-1788, and [15O]butanol). The tracer concentration in arterial blood was continuously measured to obtain the brain input function. Following image reconstruction of the scans, time–activity curves of selected regions of interest were generated. Estimations of CMRglc (1.0 ± 0.2 μmol g−1 min−1), CBF (1.4 ± 0.4 ml g−1 min−1) and transport rate constants for [ N-methyl-11C]Ro 15-1788 (K1 = 0.44 ± 0.01 ml g−1 min−1 and k2 = 0.099 ± 0.005 min−1) as well as calculated first pass extraction (0.32 ±0.1) are in reasonable agreement with literature values. Small animal studies require minimal amounts of radioactivity and can be performed without sterility and toxicology tests. They may serve as a preliminary basis for radiation safety calculations because whole body scans can be performed even with a head scanner. The major advantage of this procedure in comparison to ex vivo autoradiography is that very few experiments are necessary to reliably determine the properties of the blood–brain barrier transport of the radiotracer and the possible whole brain receptor binding characteristics.

scholarly journals In vivo tracking of tau pathology using positron emission tomography (PET) molecular imaging in small animals

2014 ◽  
Vol 3 (1) ◽  
pp. 6 ◽  
Author(s):  
Eduardo Zimmer ◽  
Antoine Leuzy ◽  
Venkat Bhat ◽  
Serge Gauthier ◽  
Pedro Rosa-Neto
Keyword(s):  
Positron Emission Tomography ◽  
Molecular Imaging ◽  
Emission Tomography ◽  
Small Animals ◽  
Tau Pathology ◽  
Positron Emission

Dynamic In Vivo Imaging of Receptors in Small Animals Using Positron Emission Tomography

2005 ◽  
pp. 217-232
Author(s):  
Peter Johnström ◽  
Tim D. Fryer ◽  
Hugh K. Richards ◽  
Olivier Barret ◽  
Anthony P. Davenport
Keyword(s):  
Positron Emission Tomography ◽  
In Vivo Imaging ◽  
Emission Tomography ◽  
Small Animals ◽  
Positron Emission

scholarly journals Development of a blood sample detector for multi-tracer positron emission tomography using gamma spectroscopy

2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Carlos Velasco ◽  
Adriana Mota-Cobián ◽  
Jesús Mateo ◽  
Samuel España
Keyword(s):  
Positron Emission Tomography ◽  
Blood Sample ◽  
Pet Imaging ◽  
Spectroscopic Analysis ◽  
Gamma Spectroscopy ◽  
Emission Tomography ◽  
Blood Samples ◽  
Positron Emission

Abstract Background Multi-tracer positron emission tomography (PET) imaging can be accomplished by applying multi-tracer compartment modeling. Recently, a method has been proposed in which the arterial input functions (AIFs) of the multi-tracer PET scan are explicitly derived. For that purpose, a gamma spectroscopic analysis is performed on blood samples manually withdrawn from the patient when at least one of the co-injected tracers is based on a non-pure positron emitter. Alternatively, these blood samples required for the spectroscopic analysis may be obtained and analyzed on site by an automated detection device, thus minimizing analysis time and radiation exposure of the operating personnel. In this work, a new automated blood sample detector based on silicon photomultipliers (SiPMs) for single- and multi-tracer PET imaging is presented, characterized, and tested in vitro and in vivo. Results The detector presented in this work stores and analyzes on-the-fly single and coincidence detected events. A sensitivity of 22.6 cps/(kBq/mL) and 1.7 cps/(kBq/mL) was obtained for single and coincidence events respectively. An energy resolution of 35% full-width-half-maximum (FWHM) at 511 keV and a minimum detectable activity of 0.30 ± 0.08 kBq/mL in single mode were obtained. The in vivo AIFs obtained with the detector show an excellent Pearson’s correlation (r = 0.996, p < 0.0001) with the ones obtained from well counter analysis of discrete blood samples. Moreover, in vitro experiments demonstrate the capability of the detector to apply the gamma spectroscopic analysis on a mixture of 68Ga and 18F and separate the individual signal emitted from each one. Conclusions Characterization and in vivo evaluation under realistic experimental conditions showed that the detector proposed in this work offers excellent sensibility and stability. The device also showed to successfully separate individual signals emitted from a mixture of radioisotopes. Therefore, the blood sample detector presented in this study allows fully automatic AIFs measurements during single- and multi-tracer PET studies.

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