scholarly journals Highly sensitive optical detection of specific protein in breast cancer cells using microstructured fiber in extremely low sample volume

2010 ◽  
Vol 15 (1) ◽  
pp. 017005 ◽  
Author(s):  
Saraswathi Padmanabhan ◽  
Vengalathunadakal K. Shinoj ◽  
Vadakke M. Murukeshan ◽  
Parasuraman Padmanabhan
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Optical Detection ◽  
Specific Protein ◽  
Sample Volume ◽  
Microstructured Fiber ◽  
Highly Sensitive

scholarly journals Aptamer Embedded Arch-Cruciform DNA Assemblies on 2-D VS2 Scaffolds for Sensitive Detection of Breast Cancer Cells

Biosensors ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 378
Author(s):  
Jinfeng Quan ◽  
Yihan Wang ◽  
Jialei Zhang ◽  
Kejing Huang ◽  
Xuemei Wang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Dna Sequences ◽  
Breast Cancer Cells ◽  
Limit Of Detection ◽  
Sensitive Detection ◽  
Highly Sensitive ◽  
Highly Sensitive Detection ◽  
Cruciform Dna ◽  
Mcf 7

Arch-cruciform DNA are self-assembled on AuNPs/VS2 scaffold as a highly sensitive and selective electrochemical biosensor for michigan cancer foundation-7 (MCF-7) breast cancer cells. In the construction, arch DNA is formed using two single-strand DNA sequences embedded with the aptamer for MCF-7 cells. In the absence of MCF-7 cells, a cruciform DNA labeled with three terminal biotin is bound to the top of arch DNA, which further combines with streptavidin-labeled horseradish peroxidase (HRP) to catalyze the hydroquinone-H2O2 reaction on the electrode surface. The presence of MCF-7 cells can release the cruciform DNA and reduce the amount of immobilized HRP, thus effectively inhibiting enzyme-mediated electrocatalysis. The electrochemical response of the sensor is negatively correlated with the concentration of MCF-7 cells, with a linear range of 10 − 1 × 105 cells/mL, and a limit of detection as low as 5 cells/mL (S/N = 3). Through two-dimensional materials and enzyme-based dual signal amplification, this biosensor may pave new ways for the highly sensitive detection of tumor cells in real samples.

scholarly journals FOXA1 directs H3K4 monomethylation at enhancers via recruitment of the methyltransferase MLL3

2016 ◽  
Author(s):  
Kamila M. Jozwik ◽  
Igor Chernukhin ◽  
Aurelien A. Serandour ◽  
Sankari Nagarajan ◽  
Jason S. Carroll
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Specific Protein ◽  
Motif Analysis ◽  
Promoter Regions ◽  
Interacting Protein ◽  
Histone Marks ◽  
Pioneer Factor ◽  
Endogenous Proteins

AbstractFOXA1 is a pioneer factor that is important in hormone dependent cancer cells to stabilise nuclear receptors, such as estrogen receptor (ER) to chromatin. FOXA1 binds to enhancers regions that are enriched in H3K4mono- and dimethylation (H3K4me1, H3K4me2) histone marks and evidence suggests that these marks are requisite events for FOXA1 to associate with enhancers to initate subsequent gene expression events. However, exogenous expression of FOXA1 has been shown to induce H3K4me1 and H3K4me2 signal at enhancer elements and the order of events and the functional importance of these events is not clear. We performed a FOXA1 Rapid Immunoprecipitation Mass Spectrometry of Endogenous Proteins (RIME) screen in ERα-positive MCF-7 breast cancer cells in order to identify FOXA1 interacting partners and we found histone-lysine N-methyltransferase (MLL3) as the top FOXA1 interacting protein. MLL3 is typically thought to induce H3K4me3 at promoter regions, but recent findings suggest it may contribute to H3K4me1 deposition, in line with our observation that MLL3 associates with an enhancer specific protein. We performed MLL3 ChIP-seq in breast cancer cells and unexpectedly found that MLL3 binds mostly at non-promoter regions enhancers, in contrast to the prevailing hypothesis. MLL3 was shown to occupy regions marked by FOXA1 occupancy and as expected, H3K4me1 and H3K4me2. MLL3 binding was dependent on FOXA1, indicating that FOXA1 recruits MLL3 to chromatin. Motif analysis and subsequent genomic mapping revealed a role for Grainy head like protein-2 (GRHL2) which was shown to co-occupy regions of the chromatin with MLL3. Regions occupied by all three factors, namely FOXA1, MLL3 and GRHL2, were most enriched in H3K4me1. MLL3 silencing decreased H3K4me1 at enhancer elements, but had no appreciable impact on H3K4me3 at enhancer elements. We identify a complex relationship between FOXA1, MLL3 and H3K4me1 at enhancers in breast cancer and propose a mechanism whereby the pioneer factor FOXA1 can interact with a chromatin modifier MLL3, recruiting it to chromatin to facilitate the deposition of H3K4me1 histone marks, subsequently demarcating active enhancer elements.

scholarly journals A proximity-labeling proteomic approach to investigate invadopodia molecular landscape in breast cancer cells

2019 ◽  
Author(s):  
Sylvie Thuault ◽  
Claire Mamelonet ◽  
Joëlle Salameh ◽  
Kevin Ostacolo ◽  
Brice Chanez ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Specific Protein ◽  
Basement Membranes ◽  
Metastatic Progression ◽  
Affinity Capture ◽  
Cytoskeleton Organization ◽  
Proteomic Approach ◽  
Molecular Landscape

ABSTRACTMetastatic progression is the leading cause of mortality in breast cancer. Invasive tumor cells develop invadopodia to travel through basement membranes and the interstitial matrix. Substantial efforts have been made to characterize invadopodia molecular composition. However, their full molecular identity is still missing due to the difficulty in isolating them. To fill this gap, we developed a non-hypothesis driven proteomic approach based on the BioID proximity biotinylation technology, using the invadopodia-specific protein Tks5α fused to the promiscuous biotin ligase BirA* as bait. In invasive breast cancer cells, Tks5α fusion concentrated to invadopodia and selectively biotinylated invadopodia components, in contrast to a fusion which lacked the membrane-targeting PX domain (Tks5β). Biotinylated proteins were isolated by affinity capture and identified by mass spectrometry. We identified known invadopodia components, revealing the pertinence of our strategy. Furthermore, we observed that Tks5 newly identified close neighbors belonged to a biologically relevant network centered on actin cytoskeleton organization. Analysis of Tks5β interactome demonstrated that some partners bound Tks5 before its recruitment to invadopodia. Thus, the present strategy allowed us to identify novel Tks5 partners that were not identified by traditional approaches and could help get a more comprehensive picture of invadopodia molecular landscape.

Highly Sensitive Single Domain Antibody–Quantum Dot Conjugates for Detection of HER2 Biomarker in Lung and Breast Cancer Cells

ACS Nano ◽  
2014 ◽  
Vol 8 (6) ◽  
pp. 5682-5695 ◽  
Author(s):  
Tatsiana Y. Rakovich ◽  
Omar K. Mahfoud ◽  
Bashir M. Mohamed ◽  
Adriele Prina-Mello ◽  
Kieran Crosbie-Staunton ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Quantum Dot ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Single Domain ◽  
Single Domain Antibody ◽  
Domain Antibody ◽  
Highly Sensitive

RNA responsive and catalytic self-assembly of DNA nanostructures for highly sensitive fluorescence detection of microRNA from cancer cells

2015 ◽  
Vol 51 (92) ◽  
pp. 16494-16497 ◽  
Author(s):  
Wenjiao Zhou ◽  
Daxiu Li ◽  
Yaqin Chai ◽  
Ruo Yuan ◽  
Yun Xiang
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Fluorescence Detection ◽  
Self Assembly ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Dna Nanostructures ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Highly Sensitive

MicroRNA triggered catalytic self-assembly of DNA nanostructures enables highly sensitive fluorescence detection of microRNA from human breast cancer cells.

scholarly journals Multifunctional Oval-Shaped Gold-Nanoparticle-Based Selective Detection of Breast Cancer Cells Using Simple Colorimetric and Highly Sensitive Two-Photon Scattering Assay

ACS Nano ◽  
2010 ◽  
Vol 4 (3) ◽  
pp. 1739-1749 ◽  
Author(s):  
Wentong Lu ◽  
Sri Ranjini Arumugam ◽  
Dulal Senapati ◽  
Anant K. Singh ◽  
Tahir Arbneshi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Gold Nanoparticle ◽  
Breast Cancer Cells ◽  
Selective Detection ◽  
Photon Scattering ◽  
Two Photon ◽  
Highly Sensitive

The proliferation of breast cancer cells are inhibited by the human intrabody targeting cyclinD1

2010 ◽  
Vol 34 (8) ◽  
pp. S49-S49
Author(s):  
Lei Wang ◽  
Xun Zhou ◽  
Lihong Zhou ◽  
Yong Chen ◽  
Xun Zhu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells
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