New method for the detection of micro-organisms in blood: application of quantitative interpretation model to aerobic blood cultures

2009 ◽  
Vol 14 (3) ◽  
pp. 034043
Author(s):  
Debra E. Huffman ◽  
Yulia M. Serebrennikova ◽  
Jennifer M. Smith ◽  
German F. Leparc ◽  
Luis H. García-Rubio
2021 ◽  
Vol 9 (6) ◽  
pp. 1170
Author(s):  
Gabriel Haddad ◽  
Sara Bellali ◽  
Tatsuki Takakura ◽  
Anthony Fontanini ◽  
Yusuke Ominami ◽  
...  

Blood culture is currently the most commonly used method for diagnosing sepsis and bloodstream infections. However, the long turn-around-time to achieve microbe identification remains a major concern for clinical microbiology laboratories. Gram staining for preliminary identification remains the gold standard. We developed a new rapid strategy using a tabletop scanning electron microscope (SEM) and compared its performance with Gram staining for the detection of micro-organisms and preliminary identification directly from blood cultures. We first optimised the sample preparation for twelve samples simultaneously, saving time on imaging. In this work, SEM proved its ability to identify bacteria and yeasts in morphotypes up to the genus level in some cases. We blindly tested 1075 blood cultures and compared our results to the Gram staining preliminary identification, with MALDI-TOF/MS as a reference. This method presents major advantages such as a fast microbe identification, within an hour of the blood culture being detected positive, low preparation costs, and data traceability. This SEM identification strategy can be developed into an automated assay from the sample preparation, micrograph acquisition, and identification process. This strategy could revolutionise urgent microbiological diagnosis of infectious diseases.


1887 ◽  
Vol 178 ◽  
pp. 113-152 ◽  

The quantitative estimation of the micro-organisms present in air is a problem which has attracted the attention of many experimenters since it received its first impetus at the hands of Pasteur twenty-six years ago. The method originally employed by Pasteur was of a very simple character, and consisted in preparing vacuous flasks of definite (1/4 litre) capacity, each containing a small quantity of nutritive liquid : the air in the flask was removed by boiling the liquid, and the open extremity was then sealed with the blowpipe. Such sealed flasks could then be preserved for an indefinite period of time without undergoing change.


1889 ◽  
Vol 15 ◽  
pp. 33-63
Author(s):  
A. B. Griffiths

In the Proceedings of the Royal Society of Edinburgh, vol. xiv. [No. 123], pp. 97–106, there is a paper of mine under the above title. I wish in the present memoir to communicate to your distinguished Society further details relative to these investigations. The principle of these researches is to find some germicidal agent capable of destroying the microbes of disease, which have been proved to reside in the blood, and are the causes (directly or indirectly) of certain contagious diseases. At the same time, an aqueous solution of such an agent, while destroying the microbes of disease, must have very little or no detrimental action upon the blood. Having found such a substance, the rationale is to inject (hypodermically) a solution of the microbe-destroyer directly into the blood. By so doing, the destruction of the pathogenic organisms in situ would be the result.


1996 ◽  
Vol 15 (7) ◽  
pp. 615-620 ◽  
Author(s):  
ATHANASIOS G. KADITIS ◽  
AENGUS S. O'MARCAIGH ◽  
K. HABLE RHODES ◽  
AMY L. WEAVER ◽  
NANCY K. HENRY

2007 ◽  
Vol 56 (2) ◽  
pp. 172-176 ◽  
Author(s):  
Anna L. Casey ◽  
Tony Worthington ◽  
Peter A. Lambert ◽  
Tom S. J. Elliott

Microbiological diagnosis of catheter-related bloodstream infection (CR-BSI) is often based on isolation of indistinguishable micro-organisms from an explanted catheter tip and blood culture, confirmed by antibiograms. Whether phenotypic identification of coagulase-negative staphylococci (CoNS) allows an accurate diagnosis of CR-BSI to be established was evaluated. Eight patients with a diagnosis of CR-BSI had CoNS isolated from pure blood cultures and explanted catheter tips which were considered as indistinguishable strains by routine microbiological methods. For each patient, an additional three colonies of CoNS isolated from the blood and five from the catheter tip were subcultured and further characterized by antibiogram profiles, analytical profile index (API) biotyping and PFGE. PFGE distinguished more strains of CoNS compared to API biotyping or antibiograms (17, 10 and 11, respectively). By PFGE, indistinguishable micro-organisms were only isolated from pure blood and catheter tip cultures in four out of eight (50 %) patients thus supporting the diagnosis of CR-BSI. In another patient, indistinguishable micro-organisms were identified in both cultures; however, other strains of CoNS were also present. The remaining three patients had multiple strains of CoNS, none of which were indistinguishable in the tip and blood cultures, thus questioning the diagnosis of CR-BSI. Phenotypic characterization of CoNS lacked discriminatory power. Current routine methods of characterizing a limited number of pooled colonies may generate misleading results as multiple strains may be present in the cultures. Multiple colonies should be studied using a rapid genotypic characterization method to confirm or refute the diagnosis of CR-BSI.


2008 ◽  
Vol 86 (5) ◽  
pp. 947-959 ◽  
Author(s):  
Jennifer M. Smith ◽  
Yulia M. Serebrennikova ◽  
Debra E. Huffman ◽  
German F. Leparc ◽  
Luis H. García-Rubio

1961 ◽  
Vol 52 (3) ◽  
pp. 489-499 ◽  
Author(s):  
I. W. B. Nye

The larvae of the Lymexylid beetle, Melittomma insulare Fairm., bore into the bases of coconut palms. Associated with them are micro-organisms which cause extensive rotting of the bole, particularly at ground-level, and this results in the palm falling.During 1953–58, an attempt was made in Praslin Island, Seychelles, to obtain maximum control of this pest using paradichlorobenzene as a fumigant. At the time of this treatment, 77 per cent, of the 90,500 coconut palms in the island were infested. Of these, about five per cent, were felled as worthless mountainside palms and nine per cent, fell during or within a week of treatment.In 1960 it was found that a further 15 per cent, of the palms had fallen and of those still standing 53 per cent, remained infested. The continuing high loss of palms after treatment was due to the closed, moist fumigation chambers within the trunks encouraging the spread of rot, coupled with an incomplete kill of larvae.A new method of treatment is described in which the necrotic wood is excised and a formulation of coal tar creosote and coal tar liberally applied. Praslin Island was retreated, using this tar method, and a plan to treat all the coconut palms in Mahé, the main island of the group, was subsequently commenced. Losses of palms during and within a week of treatment have been reduced to less than one per cent., and results are quoted which show that a single treatment can reduce a serious infestation in which 80 per cent, of palms are more or less heavily attacked to one in which only 18 per cent, of the palms are attacked and, on average, contain only five larvae. The treatment ensures that the entrances to the tunnels of these are exposed to view, and accordingly the surviving larvae can easily be killed.


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