Analysis of the fluorescence temporal point-spread function in a turbid medium and its application to optical imaging

2008 ◽  
Vol 13 (6) ◽  
pp. 064038 ◽  
Author(s):  
Sung-Ho Han ◽  
Salman Farshchi-Heydari ◽  
David J. Hall
Keyword(s):  
Optical Imaging ◽  
Point Spread Function ◽  
Turbid Medium ◽  
Point Spread ◽  
Spread Function

scholarly journals Exploiting the point spread function for optical imaging through a scattering medium based on deconvolution method

2019 ◽  
Vol 12 (04) ◽  
pp. 1930005 ◽  
Author(s):  
Hexiang He ◽  
Xiangsheng Xie ◽  
Yikun Liu ◽  
Haowen Liang ◽  
Jianying Zhou
Keyword(s):  
Optical Imaging ◽  
Point Spread Function ◽  
Optical Memory ◽  
Ease Of Use ◽  
Large Field ◽  
Depth Of Field ◽  
Deconvolution Method ◽  
Scattering Medium ◽  
Point Spread ◽  
Spread Function

Visual perception of humans penetrating turbid medium is hampered by scattering. Various techniques have been prompted recently to recover optical imaging through turbid materials. Among them, speckle correlation based on deconvolution is one of the most attractive methods taking advantage of high imaging quality, robustness, ease-of-use, and ease-of-integration. By exploiting the point spread function (PSF) of the scattering system, large Field-of-View, extended Depth-of-Field, noninvasiveness and spectral resoluation are now available as successful solutions for high quality and multifunctional image reconstruction. In this paper, we review the progress of imaging through a scattering medium based on deconvolution method, including the principle, the breakthrough of the limitation of the optical memory effect, the improvement of the deconvolution algorithm and innovative applications.

scholarly journals Nanodiamonds enable adaptive-optics enhanced, super-resolution, two-photon excitation microscopy

2019 ◽  
Vol 6 (7) ◽  
pp. 190589 ◽  
Author(s):  
Graeme E. Johnstone ◽  
Gemma S. Cairns ◽  
Brian R. Patton
Keyword(s):  
Optical Imaging ◽  
Adaptive Optics ◽  
Point Spread Function ◽  
Size Range ◽  
Resolution Enhancement ◽  
Super Resolution ◽  
Two Photon ◽  
Point Spread ◽  
Photon Excitation ◽  
Spread Function

Particles of diamond in the 5–100 nm size range, known as nanodiamond (ND), have shown promise as robust fluorophores for optical imaging. We demonstrate here that, due to their photostability, they are not only suitable for two-photon imaging, but also allow significant resolution enhancement when combined with computational super-resolution techniques. We observe a resolution of 42.5 nm when processing two-photon images with the Super-Resolution Radial Fluctuations algorithm. We show manipulation of the point-spread function of the microscope using adaptive optics. This demonstrates how the photostability of ND can also be of use when characterizing adaptive optics technologies or testing the resilience of super-resolution or aberration correction algorithms.

scholarly journals Depth estimation of the absorbing structure in a slab turbid medium using point spread function

2020 ◽  
Vol 3 (SI3) ◽  
pp. First
Author(s):  
Ngoc An Dang Nguyen ◽  
To Ni Phan Van ◽  
Kohei Yamamoto ◽  
Minh Quang Nguyen ◽  
Anh Tu Tran ◽  
...  
Keyword(s):  
Blood Vessel ◽  
Point Spread Function ◽  
Near Infrared ◽  
Fluorescent Imaging ◽  
Turbid Medium ◽  
New Technique ◽  
Infrared Light ◽  
Near Infrared Light ◽  
Point Spread ◽  
Spread Function

Nowadays, transillumination imaging is more popular used in the medical field with the development of the vein finder and the non-invasive diagnosis applications. Near-infrared light with a wavelength of 700 - 1200 nm has relatively high transmission through biological tissue. Using near-infrared light, we can able to obtain a two dimensional (2D) transillumination image of the internal absorption structure such as blood vessel structure, liver ... in the body noninvasively. Even with a simple system (light-emitting diode (LED)'s array and low-cost camera), we could obtain the blood vessel transillumination image of human arm. However, the image is severely blurred due to the strong scattering in the tissue. We have devised the depth-dependent point spread function (PSF) to suppress the scattering effect in fluorescent imaging. In previous studies, we successfully applied this principle and developed a technique to reconstruct the absorbing structure in a turbid medium without using fluorescent material. The feasibility and effectiveness of the proposed technique were verified in experiments. However, this point spread function (PSF) is depth dependence, so that the depth information is required in practice. In order to make this method more practical, the new techniques for estimating the parameters of absorbing structure (depth and width) in the turbid medium by convolution and de-convolution with the point spread function (PSF) were devised. This paper presents a new technique for the estimation depth of an absorber in 2D transillumination image. This new technique was developed to estimate the depth of the absorber in turbid medium by convolution operation with the point spread function (PSF). By observing images with two-wavelength selected at which the scattering property of the medium is different. The transillumination image at one of the wavelengths is convolved with the PSF of another wavelength. Two images of alternative wavelengths are compared while changing the depth of the PSF. We can obtain the correct depth that gives a minimum difference between the two convoluted images. This technique does not require the repetition of the unstable deconvolution operation. The effectiveness of the proposed technique was verified in simulation and experiment.

Sign in / Sign up

Export Citation Format

Share Document