scholarly journals Model-based analysis of reflectance and fluorescence spectra for in vivo detection of cervical dysplasia and cancer

2008 ◽  
Vol 13 (6) ◽  
pp. 064016 ◽  
Author(s):  
Crystal Redden Weber ◽  
Richard A. Schwarz ◽  
E. Neely Atkinson ◽  
Dennis D. Cox ◽  
Calum MacAulay ◽  
...  
Keyword(s):  
Fluorescence Spectra ◽  
Cervical Dysplasia ◽  
Model Based ◽  
In Vivo Detection ◽  
Model Based Analysis

Model-based analysis of clinical fluorescence spectroscopy for in vivo detection of cervical intraepithelial dysplasia

2006 ◽  
Vol 11 (2) ◽  
pp. 024008 ◽  
Author(s):  
Sung K. Chang ◽  
Nena Marin ◽  
Michele Follen ◽  
Rebecca Richards-Kortum
Keyword(s):  
Fluorescence Spectroscopy ◽  
Model Based ◽  
In Vivo Detection ◽  
Model Based Analysis

High Wavenumber Raman Spectroscopy for in Vivo Detection of Cervical Dysplasia

2009 ◽  
Vol 81 (21) ◽  
pp. 8908-8915 ◽  
Author(s):  
Jianhua Mo ◽  
Wei Zheng ◽  
Jeffrey J. H. Low ◽  
Joseph Ng ◽  
A. Ilancheran ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Cervical Dysplasia ◽  
In Vivo Detection

Effects of a Monoclonal Antibody to Glycoprotein IIb/IIIa (P256) and of Enzymically Derived Fragments of P256 on Human Platelets

1991 ◽  
Vol 65 (04) ◽  
pp. 432-437 ◽  
Author(s):  
A W J Stuttle ◽  
M J Powling ◽  
J M Ritter ◽  
R M Hardisty
Keyword(s):  
Monoclonal Antibody ◽  
Flow Cytometry ◽  
Platelet Aggregation ◽  
Calcium Ions ◽  
Human Platelet ◽  
Human Platelets ◽  
In Vivo Detection ◽  
Fibrinogen Binding ◽  
Specific Antagonist

SummaryThe anti-platelet monoclonal antibody P256 is currently undergoing development for in vivo detection of thrombus. We have examined the actions of P256 and two fragments on human platelet function. P256, and its divalent fragment, caused aggregation at concentrations of 10−9−3 × 10−8 M. A monovalent fragment of P256 did not cause aggregation at concentrations up to 10−7 M. P256–induced platelet aggregation was dependent upon extracellular calcium ions as assessed by quin2 fluorescence. Indomethacin partially inhibited platelet aggregation and completely inhibited intracellular calcium mobilisation. Apyrase caused partial inhibition of aggregation. Aggregation induced by the divalent fragment was dependent upon fibrinogen and was inhibited by prostacyclin. Aggregation induced by the whole antibody was only partially dependent upon fibrinogen, but was also inhibited by prostacyclin. P256 whole antibody was shown, by flow cytometry, to induce fibrinogen binding to indomethacin treated platelets. Monovalent P256 was shown to be a specific antagonist for aggregation induced by the divalent forms. In–111–labelled monovalent fragment bound to gel-filtered platelets in a saturable and displaceable manner. Monovalent P256 represents a safer form for in vivo applications

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