scholarly journals Fluorescence correlation spectroscopy and photon counting histogram on membrane proteins: functional dynamics of the glycosylphosphatidylinositol-anchored urokinase plasminogen activator receptor

2008 ◽  
Vol 13 (3) ◽  
pp. 031215 ◽  
Author(s):  
Gabriele Malengo ◽  
Annapaola Andolfo ◽  
Nicolai Sidenius ◽  
Enrico Gratton ◽  
Moreno Zamai ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Plasminogen Activator ◽  
Fluorescence Correlation Spectroscopy ◽  
Photon Counting ◽  
Correlation Spectroscopy ◽  
Urokinase Plasminogen Activator Receptor ◽  
Fluorescence Correlation ◽  
Functional Dynamics ◽  
Plasminogen Activator Receptor ◽  
Photon Counting Histogram

Investigating membrane protein dynamics in living cellsThis paper is one of a selection of papers published in this Special Issue, entitled CSBMCB — Membrane Proteins in Health and Disease.

2006 ◽  
Vol 84 (6) ◽  
pp. 825-831 ◽  
Author(s):  
Ian R. Bates ◽  
Paul W. Wiseman ◽  
John W. Hanrahan
Keyword(s):  
Membrane Proteins ◽  
Fluorescence Correlation Spectroscopy ◽  
Fluorescence Recovery After Photobleaching ◽  
Correlation Spectroscopy ◽  
Image Correlation ◽  
Fluorescence Correlation ◽  
Transport Dynamics ◽  
Health And Disease ◽  
Image Correlation Spectroscopy ◽  
Selection Of

Live cell imaging is a powerful tool for understanding the function and regulation of membrane proteins. In this review, we briefly discuss 4 fluorescence-microscopy-based techniques for studying the transport dynamics of membrane proteins: fluorescence-correlation spectroscopy, image-correlation spectroscopy, fluorescence recovery after photobleaching, and single-particle and (or) molecule tracking. The advantages and limitations of each approach are illustrated using recent studies of an ion channel and cell adhesion molecules.

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