Nonlinear optical imaging and spectral-lifetime computational analysis of endogenous and exogenous fluorophores in breast cancer

2008 ◽  
Vol 13 (3) ◽  
pp. 031220 ◽  
Author(s):  
Paolo P. Provenzano ◽  
Curtis T. Rueden ◽  
Steve M. Trier ◽  
Long Yan ◽  
Suzanne M. Ponik ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Optical Imaging ◽  
Nonlinear Optical ◽  
Computational Analysis ◽  
Nonlinear Optical Imaging ◽  
Exogenous Fluorophores

scholarly journals Nonlinear Optical Imaging of Cellular Processes in Breast Cancer

2008 ◽  
Vol 14 (6) ◽  
pp. 532-548 ◽  
Author(s):  
Paolo P. Provenzano ◽  
Kevin W. Eliceiri ◽  
Long Yan ◽  
Aude Ada-Nguema ◽  
Matthew W. Conklin ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Optical Imaging ◽  
Cancer Progression ◽  
Nonlinear Optical ◽  
Fluorescent Protein ◽  
Second Harmonic ◽  
Optical Methods ◽  
Additional Advantage ◽  
Biological Studies ◽  
Nonlinear Optical Imaging

AbstractNonlinear optical imaging techniques such as multiphoton and second harmonic generation (SHG) microscopy used in conjunction with novel signal analysis techniques such as spectroscopic and fluorescence excited state lifetime detection have begun to be used widely for biological studies. This is largely due to their promise to noninvasively monitor the intracellular processes of a cell together with the cell's interaction with its microenvironment. Compared to other optical methods these modalities provide superior depth penetration and viability and have the additional advantage in that they are compatible technologies that can be applied simultaneously. Therefore, application of these nonlinear optical approaches to the study of breast cancer holds particular promise as these techniques can be used to image exogeneous fluorophores such as green fluorescent protein as well as intrinsic signals such as SHG from collagen and endogenous fluorescence from nicotinamide adenine dinucleotide or flavin adenine dinucleotide. In this article the application of multiphoton excitation, SHG, and fluorescence lifetime imaging microscopy to relevant issues regarding the tumor-stromal interaction, cellular metabolism, and cell signaling in breast cancer is described. Furthermore, the ability to record and monitor the intrinsic fluorescence and SHG signals provides a unique tool for researchers to understand key events in cancer progression in its natural context.

Multimodal Nonlinear Optical Imaging for Sensitive Detection of Multiple Pharmaceutical Solid-State Forms and Surface Transformations

2017 ◽  
Vol 89 (21) ◽  
pp. 11460-11467 ◽  
Author(s):  
Dunja Novakovic ◽  
Jukka Saarinen ◽  
Tatu Rojalin ◽  
Osmo Antikainen ◽  
Sara J. Fraser-Miller ◽  
...  
Keyword(s):  
Solid State ◽  
Optical Imaging ◽  
Nonlinear Optical ◽  
Sensitive Detection ◽  
Nonlinear Optical Imaging

scholarly journals Multimodal Nonlinear Optical Imaging of Live Cells Using Plasmon-Coupled DNA-Mediated Gold Nanoprism Assembly

2016 ◽  
Vol 120 (8) ◽  
pp. 4546-4555 ◽  
Author(s):  
Sudarson Sekhar Sinha ◽  
Stacy Jones ◽  
Teresa Demeritte ◽  
Suhash Reddy Chavva ◽  
Yongliang Shi ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Nonlinear Optical ◽  
Live Cells ◽  
Nonlinear Optical Imaging

scholarly journals Second-Order Nonlinear Optical Imaging of Chiral Crystals

2011 ◽  
Vol 4 (1) ◽  
pp. 419-437 ◽  
Author(s):  
David J. Kissick ◽  
Debbie Wanapun ◽  
Garth J. Simpson
Keyword(s):  
Optical Imaging ◽  
Nonlinear Optical ◽  
Second Order ◽  
Nonlinear Optical Imaging ◽  
Chiral Crystals

scholarly journals Coherent Nonlinear Optical Imaging: Beyond Fluorescence Microscopy

2011 ◽  
Vol 62 (1) ◽  
pp. 507-530 ◽  
Author(s):  
Wei Min ◽  
Christian W. Freudiger ◽  
Sijia Lu ◽  
X. Sunney Xie
Keyword(s):  
Fluorescence Microscopy ◽  
Optical Imaging ◽  
Nonlinear Optical ◽  
Nonlinear Optical Imaging

ChemInform Abstract: Nonlinear Optical Imaging - Introduction and Pharmaceutical Applications

ChemInform ◽  
2014 ◽  
Vol 45 (34) ◽  
pp. no-no
Author(s):  
Andrew L. Fussell ◽  
Antti Isomaki ◽  
Clare J. Strachan
Keyword(s):  
Optical Imaging ◽  
Nonlinear Optical ◽  
Pharmaceutical Applications ◽  
Nonlinear Optical Imaging

Molecular probes for nonlinear optical imaging of biological membranes

2001 ◽  
Author(s):  
Mireille H. Blanchard-Desce ◽  
Lionel Ventelon ◽  
Sandrine Charier ◽  
Laurent Moreaux ◽  
Jerome Mertz
Keyword(s):  
Optical Imaging ◽  
Nonlinear Optical ◽  
Biological Membranes ◽  
Molecular Probes ◽  
Nonlinear Optical Imaging
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