Detection of interproximal demineralized lesions on human teeth in vitro using frequency-domain infrared photothermal radiometry and modulated luminescence

2007 ◽  
Vol 12 (3) ◽  
pp. 034028 ◽  
Author(s):  
Raymond J. Jeon ◽  
Anna Matvienko ◽  
Andreas Mandelis ◽  
Stephen H. Abrams ◽  
Bennett T. Amaechi ◽  
...  
Keyword(s):  
Frequency Domain ◽  
Photothermal Radiometry ◽  
Human Teeth

scholarly journals Dental Pulp-Derived Mesenchymal Stem Cells for Modeling Genetic Disorders

2021 ◽  
Vol 22 (5) ◽  
pp. 2269
Author(s):  
Keiji Masuda ◽  
Xu Han ◽  
Hiroki Kato ◽  
Hiroshi Sato ◽  
Yu Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Dental Pulp ◽  
Genetic Disorders ◽  
In Vitro Models ◽  
High Plasticity ◽  
Human Teeth ◽  
Clinical Diagnoses ◽  
Models Of Disease

A subpopulation of mesenchymal stem cells, developmentally derived from multipotent neural crest cells that form multiple facial tissues, resides within the dental pulp of human teeth. These stem cells show high proliferative capacity in vitro and are multipotent, including adipogenic, myogenic, osteogenic, chondrogenic, and neurogenic potential. Teeth containing viable cells are harvested via minimally invasive procedures, based on various clinical diagnoses, but then usually discarded as medical waste, indicating the relatively low ethical considerations to reuse these cells for medical applications. Previous studies have demonstrated that stem cells derived from healthy subjects are an excellent source for cell-based medicine, tissue regeneration, and bioengineering. Furthermore, stem cells donated by patients affected by genetic disorders can serve as in vitro models of disease-specific genetic variants, indicating additional applications of these stem cells with high plasticity. This review discusses the benefits, limitations, and perspectives of patient-derived dental pulp stem cells as alternatives that may complement other excellent, yet incomplete stem cell models, such as induced pluripotent stem cells, together with our recent data.

scholarly journals Remineralization of natural early caries lesions in vitro by P11-4 monitored with photothermal radiometry and luminescence

2017 ◽  
Vol 8 (4) ◽  
pp. e12257 ◽  
Author(s):  
Joshua D. Silvertown ◽  
Bonny P. Y. Wong ◽  
Koneswaran S. Sivagurunathan ◽  
Stephen H. Abrams ◽  
Jennifer Kirkham ◽  
...  
Keyword(s):  
Photothermal Radiometry ◽  
Early Caries ◽  
Caries Lesions

scholarly journals The Antibacterial Efficacy of Biopure MTAD in Root Canal Contaminated with Enterococcus faecalis

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Blerim Kamberi ◽  
Donika Bajrami ◽  
Miranda Stavileci ◽  
Shuhreta Omeragiq ◽  
Fatmir Dragidella ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Sodium Hypochlorite Solution ◽  
Antibacterial Efficacy ◽  
Human Teeth ◽  
Exact Test ◽  
Hypochlorite Solution ◽  
Root Canals ◽  
Significant Difference ◽  
Level Of Significance

Aim. The purpose of this in vitro study was to assess the antimicrobial efficacy of Biopure MTAD against E. faecalis in contaminated root canals. Materials and Methods. Forty-two single rooted extracted human teeth were inoculated with E. faecalis and incubated for four weeks. The samples were divided in two control and five experimental groups irrigated with 1.5% sodium hypochlorite solution (NaOCl); 3% NaOCl; BioPure MTAD; 1.5% NaOCl/17% EDTA; or 3% NaOCl/17% EDTA. After a one-week incubation, complete disinfection was confirmed by the absence of turbidity in the incubation media. Dentin shavings were taken from samples with no turbidity to verify whether E. faecalis was present in dentin tubules. Results were analyzed statistically using Fisher's exact test, with the level of significance set at . Results. Statistical analysis of the data obtained at Day 7 and after dentin shaving analysis showed that BioPure MTAD had significantly greater antibacterial activity than 1.5% NaOCl, 1.5% NaOCl/17% EDTA and 3% NaOCl/17% EDTA. No significant difference was detected between MTAD and 3% NaOCl. Conclusions. These findings suggest that BioPure MTAD possesses superior bactericidal activity compared with NaOCl and EDTA against E. faecalis.

scholarly journals Effectiveness of a silicon-based root canal sealer for filling of simulated lateral canals

2007 ◽  
Vol 18 (1) ◽  
pp. 20-23 ◽  
Author(s):  
João Vicente Baroni Barbizam ◽  
Matheus Souza ◽  
Doglas Cecchin ◽  
Jakob Dabbel
Keyword(s):  
Root Canal ◽  
In Vitro Study ◽  
Root Canal Sealer ◽  
Human Teeth ◽  
Significance Level ◽  
Working Length ◽  
Group 3 ◽  
Group 2 ◽  
Silicon Based

The purpose of this in vitro study was to evaluate the ability of a silicon-based root canal sealer, compared to zinc oxide and eugenol and an epoxy resin-based sealers, for filling of simulated lateral canals. Thirty extracted single-rooted human teeth were selected, conventional access was made and the working length was established 1 mm from the apical foramen. Three simulated lateral canals, one in each root third (coronal, middle and apical) were prepared in both the mesial and distal surfaces of each tooth using a size 15 reamer adapted to a low-speed handpiece. Each root canal was instrumented using ProTaper rotary files up to file F3 at the working length, and then irrigated with 2.5% NaOCl followed by EDTA. The teeth were assigned to 3 groups (n=10), according to the root canal sealer: Roeko Seal (Group 1), Sealer 26 (group 2) and Grossman's sealer (Group 3). Gutta-percha cold lateral condensation technique was performed in all groups. Postoperative radiographs were taken and the images were projected for evaluation of the quality of lateral canal filling. Data were submitted to statistical analysis by Kruskal Wallis test at 5% significance level. The results showed that Grossman's sealer filled a larger number of lateral canals than Roeko Seal (p<0.05) and Sealer 26 (p<0.01). It may be concluded that Roeko Seal silicone-based root canal sealer was not as effective as the Grossman's sealer for filling of simulated lateral canals. The lateral canals localized in the apical third of the root were more difficult to be filled.

scholarly journals Wave Propagation in Thin-Walled Aortic Analogues

2010 ◽  
Vol 132 (2) ◽  
Author(s):  
C. G. Giannopapa ◽  
J. M. B. Kroot ◽  
A. S. Tijsseling ◽  
M. C. M. Rutten ◽  
F. N. van de Vosse
Keyword(s):  
Experimental Data ◽  
Wave Propagation ◽  
Frequency Domain ◽  
Analytical Model ◽  
Viscoelastic Properties ◽  
Numerical Models ◽  
Computational Cost ◽  
One Dimensional ◽  
Arterial Blood

Research on wave propagation in liquid filled vessels is often motivated by the need to understand arterial blood flows. Theoretical and experimental investigation of the propagation of waves in flexible tubes has been studied by many researchers. The analytical one-dimensional frequency domain wave theory has a great advantage of providing accurate results without the additional computational cost related to the modern time domain simulation models. For assessing the validity of analytical and numerical models, well defined in vitro experiments are of great importance. The objective of this paper is to present a frequency domain analytical model based on the one-dimensional wave propagation theory and validate it against experimental data obtained for aortic analogs. The elastic and viscoelastic properties of the wall are included in the analytical model. The pressure, volumetric flow rate, and wall distention obtained from the analytical model are compared with experimental data in two straight tubes with aortic relevance. The analytical results and the experimental measurements were found to be in good agreement when the viscoelastic properties of the wall are taken into account.

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