Age-related structural abnormalities in the human retina-choroid complex revealed by two-photon excited autofluorescence imaging

2007 ◽  
Vol 12 (2) ◽  
pp. 024012 ◽  
Author(s):  
Meng Han ◽  
Guenter Giese ◽  
Steffen Schmitz-Valckenberg ◽  
Almut Bindewald-Wittich ◽  
Frank G. Holz ◽  
...  
Keyword(s):  
Human Retina ◽  
Autofluorescence Imaging ◽  
Two Photon ◽  
Age Related ◽  
Structural Abnormalities

Age-Related Changes in the Human Retina

2006 ◽  
pp. 793-812 ◽  
Author(s):  
Carlo Cavallotti ◽  
Nicola Pescosolido
Keyword(s):  
Human Retina ◽  
Age Related ◽  
Age Related Changes

scholarly journals Two-Photon Autofluorescence Imaging Reveals Cellular Structures Throughout the Retina of the Living Primate Eye

2016 ◽  
Vol 57 (2) ◽  
pp. 632 ◽  
Author(s):  
Robin Sharma ◽  
David R. Williams ◽  
Grazyna Palczewska ◽  
Krzysztof Palczewski ◽  
Jennifer J. Hunter
Keyword(s):  
Cellular Structures ◽  
Autofluorescence Imaging ◽  
Two Photon

Age-related changes in the human retina

2004 ◽  
Vol 39 (1) ◽  
pp. 61-68 ◽  
Author(s):  
Carlo Cavallotti ◽  
Marco Artico ◽  
Nicola Pescosolido ◽  
Francesca Maria Tranquilli Leali ◽  
Janos Feher
Keyword(s):  
Human Retina ◽  
Age Related ◽  
Age Related Changes

scholarly journals Wide Field Imaging and Angiography

2017 ◽  
pp. 32-37
Keyword(s):  
Age Related Macular Degeneration ◽  
Peripheral Retina ◽  
Management Approach ◽  
Wide Field ◽  
Autofluorescence Imaging ◽  
Retinal Periphery ◽  
Age Related ◽  
Increased Risk ◽  
Field Imaging ◽  
Wide Field Imaging

The recently developed wide-field imaging systems (WIS) enable 200 degrees imaging of retina by just one shot and angiographic and autofluorescence imaging of retinal periphery. In addition to facilitating the detection of retinal pathologies, WIS revealed the importance of lesions in retinal periphery and peripheral nonperfusion areas invisible by standard angiography. The novel finding of utmost importance for diabetic retinopathy (DR) patients was the 3 fold increased risk of progression assessed for peripheral DR lesions compared to that in the posterior pole. Detection of peripheral nonperfusion areas in DR or retinal vein occlusion cases with refractory macular edema altered the management approach. On the other hand by means of WIS new terms have been brought into the era like peripheral vascular leakage whose relevance and importance are unknown for retinal vascular diseases. In age-related macular degeneration, a disease known to affect the macula, WIS proved the effect on peripheral retina documented by both angiographies and also peripheral autofluorescence. In diseases known to affect peripheral retina like uveitis, degenerative myopia, etc. WIS further contributes to the detection of additional pathologies. WIS enabled documentation of retinal periphery and thus seems to change the treatment modality and probably clinical classification of unknown or treatment-refractory pathologies in retinal diseases where we mainly focused on macula always.

scholarly journals Distinct patterns of activity in individual cortical neurons and local networks in primary somatosensory cortex of mice evoked by mechanical limb stimulation

2020 ◽  
Author(s):  
Mischa V. Bandet ◽  
Bin Dong ◽  
Ian R. Winship
Keyword(s):  
Somatosensory Cortex ◽  
Cortical Neurons ◽  
Optical Recording ◽  
Primary Somatosensory Cortex ◽  
Autofluorescence Imaging ◽  
Neuronal Activation ◽  
Local Networks ◽  
Two Photon ◽  
Evoked Activity ◽  
Sensory Evoked

AbstractTo distinguish between somatic stimuli, the primary somatosensory cortex should process dissimilar stimuli with distinct patterns of neuronal activation. Two-photon calcium imaging permits simultaneous optical recording of sensory evoked activity in hundreds of cortical neurons during varied sensory stimulation. Hence, it allows a visualization of patterns of activity in individual neurons and local cortical networks in response to distinct stimulation. Here, flavoprotein autofluorescence imaging was used to map the somatosensory cortex of anaesthetized C57BL/6 mice, and in vivo two-photon Ca2+ imaging was used to define patterns of neuronal activation during mechanical stimulation of the contralateral forelimb or hindlimb at various frequencies (3, 10, 100, 200, and 300 Hz). The data revealed that neurons within the limb associated somatosensory cortex exhibit stimulus-specific patterns of activity. Subsets of neurons were found to have sensory-evoked activity that is either primarily responsive to single stimulus frequencies or broadly responsive to multiple frequencies of limb movement. High frequency stimuli were shown to elicit more activation across the population, with a greater percentage of the population responding and greater percentage of cells with high amplitude responses. Stimulus-evoked cell-cell correlations within these neuronal networks varied as a function of frequency of stimulation, such that each stimulus elicited a distinct pattern that was more consistent across multiple trials of the same stimulus compared to trials at different frequencies of stimulation. The variation in cortical response to these artificial stimuli can thus be represented by the population pattern of supra-threshold Ca2+ transients, the magnitude and temporal properties of the evoked activity, and the structure of the stimulus-evoked correlation between responsive neurons.

Best Clinical Practice for Age-Related Macular Degeneration Imaging

2019 ◽  
Vol 3 (3) ◽  
pp. 167-171 ◽  
Author(s):  
K. Bailey Freund ◽  
Cynthia Toth ◽  
Marco Zarbin
Keyword(s):  
Optical Coherence Tomography ◽  
Macular Degeneration ◽  
Fundus Autofluorescence ◽  
Age Related Macular Degeneration ◽  
Fundus Photography ◽  
Optical Coherence ◽  
Autofluorescence Imaging ◽  
Clinical Practices ◽  
Age Related

Purpose: To identify best clinical practices for macular degeneration imaging. Methods: We reviewed best clinical practices for imaging patients with age-related macular degeneration. These recommendations are based on different levels of evidence (I-III). Results: The type of imaging needed depends to some degree on the clinical scenario: first visit vs follow-up visit vs poorly responsive patient. Conclusions: Imaging technologies that may be useful include optical coherence tomography, fundus photography, fundus autofluorescence imaging, fluorescein angiography, indocyanine green angiography, and optical coherence tomography angiography.

Sign in / Sign up

Export Citation Format

Share Document