Fiber optic biosensor for the detection of C-reactive protein and the study of protein binding kinetics

2007 ◽  
Vol 12 (2) ◽  
pp. 024025 ◽  
Author(s):  
Chien Chou ◽  
Hsien-Yeh Hsu ◽  
Hsieh-Ting Wu ◽  
Kai-Yu Tseng ◽  
Arthur Chiou ◽  
...  
Keyword(s):  
Protein Binding ◽  
Fiber Optic ◽  
Binding Kinetics ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Protein Binding Kinetics

Fiber optic biosensor for monitoring protein binding kinetics

2005 ◽  
Author(s):  
Chih-Jen Yu ◽  
Chien Chou ◽  
Hsien-Yeh Hsu ◽  
Tsu-Shin Chan ◽  
Zheng-Yuan Lee ◽  
...  
Keyword(s):  
Protein Binding ◽  
Fiber Optic ◽  
Binding Kinetics ◽  
Protein Binding Kinetics

scholarly journals Nanofluidic Fluorescence Microscopy (NFM) for real-time monitoring of protein binding kinetics and affinity studies

2017 ◽  
Vol 88 ◽  
pp. 25-33 ◽  
Author(s):  
Pattamon Teerapanich ◽  
Martine Pugnière ◽  
Corinne Henriquet ◽  
Yii-Lih Lin ◽  
Chia-Fu Chou ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Protein Binding ◽  
Real Time ◽  
Binding Kinetics ◽  
Real Time Monitoring ◽  
Protein Binding Kinetics

scholarly journals A Multiscale Simulation Approach to Modeling Drug–Protein Binding Kinetics

2018 ◽  
Vol 14 (11) ◽  
pp. 6093-6101 ◽  
Author(s):  
Susanta Haldar ◽  
Federico Comitani ◽  
Giorgio Saladino ◽  
Christopher Woods ◽  
Marc W. van der Kamp ◽  
...  
Keyword(s):  
Protein Binding ◽  
Multiscale Simulation ◽  
Binding Kinetics ◽  
Simulation Approach ◽  
Protein Binding Kinetics ◽  
Drug Protein Binding

A Label-Free Untethered Approach to Single-Molecule Protein Binding Kinetics

Nano Letters ◽  
2014 ◽  
Vol 14 (10) ◽  
pp. 5787-5791 ◽  
Author(s):  
Ahmed A. Al Balushi ◽  
Reuven Gordon
Keyword(s):  
Protein Binding ◽  
Single Molecule ◽  
Binding Kinetics ◽  
Label Free ◽  
Protein Binding Kinetics

Peritoneal Homocysteine Clearance is Inefficient in Peritoneal Dialysis Patients

2000 ◽  
Vol 20 (6) ◽  
pp. 766-771 ◽  
Author(s):  
David W. Johnson ◽  
Troy D. Kay ◽  
David A. Vesey ◽  
Nicole Isbel ◽  
Scott B. Campbell ◽  
...  
Keyword(s):  
Peritoneal Dialysis ◽  
Protein Binding ◽  
Plasma Protein Binding ◽  
Plasma Protein ◽  
Tertiary Care ◽  
Plasma Concentrations ◽  
Elevated Plasma ◽  
C Reactive Protein ◽  
Protein Loss ◽  
Reactive Protein

Objectives To investigate the degree and the determinants of peritoneal homocysteine (Hcy) clearance and to compare measured Hcy clearance with the Hcy clearance predicted based on molecular weight (MW). Design Cross-sectional observational analysis. Setting Tertiary care institutional dialysis center. Patients Sixty-five stable peritoneal dialysis (PD) patients. Outcome Measures Fasting blood and 24-hour pooled dialysate effluents were collected for determination of peritoneal clearances of Hcy (CpHcy), urea (CpUr), and creatinine (CpCr). The dialysate-to-plasma creatinine ratio at 4 hours (D/P Cr 4 h) and levels of red cell folate, B12, ferritin, and C-reactive protein (CRP) were measured concurrently. Observed CpHcy was compared with predicted clearance, based on Hcy plasma protein binding and the relative molecular weights of Hcy, urea, and creatinine. Results Plasma concentrations of Hcy averaged 24.6 ± 1.1 μmol/L and were elevated above the upper limit of normal in 59 (91%) patients. The mean dialysate concentration of Hcy was 2.9 ± 0.3 μmol/L, equating to a daily peritoneal elimination of 34.6 ± 3.6 μmol. Observed CpHcy was closely approximated by predicted CpHcy (8.7 ± 0.6 L/week/1.73 m2 vs 9.0 ± 0.3 L/week/1.73 m2 respectively, p = 0.55). Patients maintained on automated PD ( n = 5) had a CpHcy similar to that of patients treated with continuous ambulatory peritoneal dialysis (8.9 ± 1.0 L/week/1.73 m2 vs 8.7 ± 0.6 L/week/1.73 m2, p = 0.92). The CpHcy was significantly correlated with C-reactive protein (CRP), D/P creatinine, CpUr, CpCr, and peritoneal protein loss, but not with plasma Hcy, albumin, B12, ferritin, age, dialysis duration, peritonitis episodes, or daily dialysate effluent volume. By multivariate analysis, the only variables that remained significant independent predictors of CpHcy were CRP and D/P Cr 4 h. High and high-average transporters had a higher CpHcy than low and low-average transporters (9.7 ± 0.8 L/week/1.73 m2 vs 7.0 ± 0.7 L/week/1.73 m2, p < 0.05), despite comparably elevated plasma Hcy concentrations [25.2 ± 1.5 μmol/L vs 23.4 ± 1.6 μmol/L, p = nonsignificant (NS)]. Conclusions Elevated plasma concentrations of Hcy are not efficiently reduced by PD. The relatively low peritoneal clearance of Hcy is largely accounted for by a high degree of plasma protein binding and is significantly influenced by peritoneal membrane permeability.

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