New fluorescence imaging probe with high spatial resolution for in vivo applications

2004 ◽  
Vol 9 (5) ◽  
pp. 928 ◽  
Author(s):  
V. Bonnans ◽  
T. Gharbi ◽  
C. Pieralli ◽  
B. Wacogne ◽  
Ph. Humbert
Keyword(s):  
Fluorescence Imaging ◽  
Spatial Resolution ◽  
High Spatial Resolution ◽  
Imaging Probe

scholarly journals In vivo fluorescence imaging with a flat, lensless microscope

2020 ◽  
Author(s):  
Jesse K. Adams ◽  
Vivek Boominathan ◽  
Sibo Gao ◽  
Alex V. Rodriguez ◽  
Dong Yan ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Spatial Resolution ◽  
High Spatial Resolution ◽  
Animal Movement ◽  
Brain Activity ◽  
Phase Mask ◽  
Reconstruction Algorithms ◽  
Lensless Imaging ◽  
Computational Reconstruction

AbstractFluorescence imaging over large areas of the brain in freely behaving animals would allow researchers to better understand the relationship between brain activity and behavior; however, traditional microscopes capable of high spatial resolution and large fields of view (FOVs) require large and heavy lenses that restrict animal movement. While lensless imaging has the potential to achieve both high spatial resolution and large FOV with a thin lightweight device, lensless imaging has yet to be achieved in vivo due to two principal challenges: (a) biological tissue typically has lower contrast than resolution targets, and (b) illumination and filtering must be integrated into this non-traditional device architecture. Here, we show that in vivo fluorescence imaging is possible with a thin lensless microscope by optimizing the phase mask and computational reconstruction algorithms, and integrating fiber optic illumination and thin-film color filters. The result is a flat, lensless imager that achieves better than 10 μm spatial resolution and a FOV that is 30× larger than other cellular resolution miniature microscopes.

Interphalangeal Joint Cartilage: High-Spatial-Resolution in Vivo MR T2 Mapping—A Feasibility Study

Radiology ◽  
2004 ◽  
Vol 233 (1) ◽  
pp. 292-296 ◽  
Author(s):  
Jelena Lazovic-Stojkovic ◽  
Timothy J. Mosher ◽  
Harvey E. Smith ◽  
Qing X. Yang ◽  
Bernard J. Dardzinski ◽  
...  
Keyword(s):  
Feasibility Study ◽  
Spatial Resolution ◽  
High Spatial Resolution ◽  
T2 Mapping ◽  
Interphalangeal Joint ◽  
Joint Cartilage

scholarly journals A Novel TMTP1-modified Theranostic Nanoplatform for Targeted in Vivo NIR-II Fluorescence Imaging-guided Chemotherapy of Cervical Cancer

2021 ◽  
Author(s):  
Alifu Nuernisha ◽  
Rong Ma ◽  
Lijun Zhu ◽  
Zhong Du ◽  
Shuang Chen ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Blood Vessels ◽  
Fluorescence Imaging ◽  
Spatial Resolution ◽  
Self Assembly ◽  
Tumor Targeting ◽  
High Sensitivity ◽  
High Definition ◽  
Targeting Ability

Abstract BackgroundNear-infrared II (NIR-II, 900-1700 nm) fluorescence bioimaging with advantages of good biosafety, excellent spatial resolution, high sensitivity and contrast, has attracted great attentions in biomedical research fields. However, most nanoprobes used for NIR-II fluorescence imaging have poor tumor-targeting ability and therapeutic efficiency. To overcome these limitations, a novel NIR-II-emissive theranostic nanoplatform for imaging and treatment of cervical cancer was designed and prepared. The NIR-II-emissive dye IR-783 and chemotherapy drug doxorubicin (DOX) were encapsulated into liposomes, and the tumor-targeting peptide TMTP1 was conjugated to the surface of the liposomes to form IR-783-DOX-TMTP1 nanoparticles (NPs) via self-assembly methods.ResultsThe IR-783-DOX-TMTP1 NPs showed strong NIR-II emission, excellent biocompatibility, a long lifetime, and low toxicity. Further, high-definition NIR-II fluorescence microscopy images of ear blood vessels and intratumor blood vessels were obtained from IR-783-DOX-TMTP1 NPs-stained mice with high spatial resolution under 808 nm laser excitation. Moreover, IR-783-DOX-TMTP1 NPs showed strong tumor targeting ability and high efficiently chemotherapeutic character towards cervical tumors. ConclusionsThe novel targeting and NIR-II-emissive IR-783-DOX-TMTP1 NPs have potential in diagnosis and therapy for cervical cancer.

In vivo multi-photon luminescence imaging of cerebral vasculature and blood–brain barrier integrity using gold nanoparticles

2015 ◽  
Vol 3 (15) ◽  
pp. 2935-2938 ◽  
Author(s):  
Hwan-Jun Yoon ◽  
Eek-Sung Lee ◽  
Minseok Kang ◽  
Yong Jeong ◽  
Ji-Ho Park
Keyword(s):  
Gold Nanoparticles ◽  
Spatial Resolution ◽  
High Spatial Resolution ◽  
Morphological Changes ◽  
Time Dependent ◽  
Cerebral Vasculature ◽  
Barrier Integrity ◽  
Cerebral Microvasculature ◽  
Blood Brain Barrier Integrity

Time-dependent morphological changes of cerebral microvasculature can be visualized with high spatial resolution using the multi-photon luminescence of gold nanoparticles.

scholarly journals Micro-endoscope for in vivo widefield high spatial resolution fluorescent imaging

2012 ◽  
Vol 3 (6) ◽  
pp. 1274 ◽  
Author(s):  
C D Saunter ◽  
S. Semprini ◽  
C. Buckley ◽  
J Mullins ◽  
J M Girkin
Keyword(s):  
Spatial Resolution ◽  
High Spatial Resolution ◽  
Fluorescent Imaging

scholarly journals Protein enhanced NIR-IIb emission of indocyanine green for functional bioimaging

2020 ◽  
Author(s):  
Mubin He ◽  
Di Wu ◽  
Yuhuang Zhang ◽  
Xiaoxiao Fan ◽  
Hui Lin ◽  
...  
Keyword(s):  
Indocyanine Green ◽  
Fluorescence Imaging ◽  
Clinical Application ◽  
Spatial Resolution ◽  
Near Infrared ◽  
Protein Solutions ◽  
Lymph System ◽  
Fetal Bovine ◽  
Auto Fluorescence

AbstractFluorescence imaging performed in the 1500-1700 nm spectral range (labeled as near-infrared IIb, NIR-IIb) promises high imaging contrast and spatial resolution for its little photon scattering effect and minimum auto-fluorescence. Though inorganic and organic probes have been developed for NIR-IIb bioimaging, most are in preclinical stage, hampering further clinical application. Herein, we showed that indocyanine green (ICG), an US Food and Drug Administration (FDA)-approved agent, exhibited remarkable amount of NIR-IIb emission when dissolved into different protein solutions, including human serum albumin, rat bile, and fetal bovine serum. We performed fluorescence imaging in NIR-IIb window to visualize structures of lymph system, extrahepatic biliary tract and cerebrovascular. Results demonstrated that proteins promoted NIR-IIb emission of ICG in vivo and that NIR-IIb imaging with ICG preserved higher signal-to-background ratio (SBR) and spatial resolution compared with the conventional near-infrared II (NIR-II) fluorescence imaging. Our findings confirm that NIR-IIb fluorescence imaging can be successfully performed using the clinically approved agent ICG. Further clinical application in NIR-IIb region would hopefully be carried out with appropriate ICG-protein solutions.

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