scholarly journals Reproductive cycle of male wall lizard, Podarcis vaucheri (Reptilia: Sauria: Lacertidae), in Djurdjura, Northern Algeria

2017 ◽  
Author(s):  
Rabah Mamou ◽  
Elara Moudilou ◽  
Jean-Marie Exbrayat ◽  
Mansour Amroun
Keyword(s):  
Sexual Activity ◽  
Reproductive Cycle ◽  
Mixed Type ◽  
Progressive Increase ◽  
Early Summer ◽  
Seminiferous Tubules ◽  
Testis Weight ◽  
Field Observations ◽  
Testicular Weight ◽  
Resting Period

The reproductive cycle of male wall lizard Podarcis vaucheri (Boulenger, 1905) living in Djurdjura mountain, was analyzed from April till October 2014. Its seasonal mixed-type cycle was characterized by three phases: sexual activity, quiescence and recrudescence. After the emergence of lizard from hibernation an intense sexual activity was observed in spring (April, May, and June) at which the testicular weight and seminiferous tubules diameter reached their maximal values. At this period, spermiogenesis was also maximal and the spermiation allowed the release of a large quantity of spermatozoa in both the seminiferous tubules and epididymis lumen. In July, a short sexual resting period followed, during which the testes mass and seminiferous tubules diameter decreased. Spermatogonia and some primary spermatocytes were observed against the wall of seminiferous tubules. The recrudescence period (August, September and October) was characterized by a progressive increase of the testis weight and seminiferous tubules diameter. Spermatocytogenesis began in August, and spermiogenesis occurred in September and October, before the diapause period. But the presence of spermatozoids was not signaled in the epididymis lumen. Field observations and microscopic evidence of testis showed that both sexes reproduced synchronously during spring and early summer.

Secretion of transferrin in ovine seminiferous tubule cell cultures in response to FSH: influence of breed, season of birth and age of lambs

1993 ◽  
Vol 5 (1) ◽  
pp. 83 ◽  
Author(s):  
C Monet-Kuntz ◽  
I Fontaine
Keyword(s):  
Cell Cultures ◽  
Dose Response ◽  
Sertoli Cells ◽  
Seminiferous Tubule ◽  
Seminiferous Tubules ◽  
Testis Weight ◽  
Season Of Birth ◽  
Tubule Cell ◽  
Response Curves ◽  
Testicular Weight

The response of lamb Sertoli cells to follicle stimulating hormone (FSH) was investigated by measuring transferrin secretion in seminiferous tubule cell cultures throughout the non-pubertal and the prepubertal periods. Cells could be cultured from birth until they attained a testicular weight of 19 g. The characteristics of individual dose-response curves were compared according to the breed, season of birth and testicular weight of the lambs. At the same season of birth and within a given testis weight range, dose-response curves of Romanov and Ile-de-France lambs were similar. Within a given testis weight range, spring-born animals exhibited a higher maximal transferrin secretion than autumn-born lambs, but the ED50 was similar. The main factor of variation of the dose-response curve parameters was the testicular weight of the lambs: the amplitude of FSH response increased 3-fold from a testicular weight of 6 g onwards, i.e. from the appearance of spermatogonia in seminiferous tubules. The ED50 increased 5-fold from 11 g onwards, i.e. from the beginning of the prepubertal period. Thus, Sertoli cells become less sensitive to FSH as spermatogenesis develops in seminiferous tubules. This phenomenon is largely the result of higher phosphodiesterase activity and is greatly reduced by 1-methyl-3-isobutyl-xanthine (MIX).

scholarly journals PERMEABILITY OF SERTOLI CELL TIGHT JUNCTIONS TO LANTHANUM AFTER LIGATION OF DUCTUS DEFERENS AND DUCTULI EFFERENTES

1973 ◽  
Vol 59 (3) ◽  
pp. 559-572 ◽  
Author(s):  
William B. Neaves
Keyword(s):  
Tight Junctions ◽  
Sertoli Cell ◽  
Seminiferous Tubules ◽  
Testis Weight ◽  
Intercellular Spaces ◽  
Ductus Deferens ◽  
Ductuli Efferentes ◽  
Testicular Weight ◽  
En Face ◽  
Efferent Ductule

The permeability of Sertoli cell tight junctions to lanthanum administered during fixation has been compared in rats after ligation of the ductus deferens and after ligation of the ductuli efferentes. In both control and vasoligated testes, lanthanum penetrated only short distances into the Sertoli cell tight junctions before stopping abruptly. The tight junction, consisting of numerous pentalaminar fusions of contiguous Sertoli cell membranes, prevented diffusion of lanthanum into the adluminal compartment of the seminiferous epithelium. In rats with ligated ductuli efferentes, lanthanum completely permeated many Sertoli cell tight junctions and occupied intercellular spaces of the adluminal compartment. In spite of their newly acquired permeability to lanthanum, tight junctions retained characteristic ultrastructural features, including numerous membrane fusions. When lanthanum-filled tight junctions were sectioned en face, membrane fusions appeared as pale lines in lakes of electron-opaque tracer. These linearly extensive fasciae occludentes occasionally ended blindly, suggesting that lanthanum may have traversed the junction by diffusing around such incomplete barriers. The increased permeability of Sertoli cell tight junctions after efferent ductule ligation, which caused rapid testicular weight gain followed by atrophy, indicates that tight junctions are sensitive to enforced retention of testicular secretions inside the seminiferous tubules. The apparent normalcy of Sertoli cell tight junctions after vasoligation, which had no effect on testis weight, supports the view that blockage of testicular secretions distal to the epididymis is relatively innocuous.

scholarly journals Testicular and seminal evaluation of goats fed hay Cenostigma pyramidale

2020 ◽  
Vol 40 (12) ◽  
pp. 963-969
Author(s):  
Maria V.B. Santos ◽  
Ana K.S. Cavalcante ◽  
Juliana T.S.A. Macêdo ◽  
Marilúcia C. Santos ◽  
Laiara F. Rocha ◽  
...  
Keyword(s):  
Gonadosomatic Index ◽  
Live Weight ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Blood Collection ◽  
Panicum Maximum ◽  
Alternative Source ◽  
Parametric Data ◽  
Testicular Weight ◽  
Student’S T

ABSTRACT: This study aimed to assess the possible occurrence of reproductive changes in male goats associated with ingestion of Cenostigma pyramidale hay. Sixteen animals divided into two experimental groups, G1 and G2 (control group) were used. Animals in G1 received 2% of forage, based on live weight (LW), composed of 100% of C. pyramidale, and animals in G2 received 2% of Panicum maximum “Massai’ grass hay, based on LW. Both groups received 1% of concentrated feed supplementation based on LW, along with mineralized salt and water ad libitum. The goats were subjected to weighing, testicular biometry, and semen and blood collection every 30 days. After 120 days, the animals were castrated and their testes were collected. Testicular measurements were performed and fragments were collected for histological processing to determine the gonadosomatic index (GSI), diameter of the seminiferous tubules, height of the germinal epithelium (HGE), volumetric proportion and volume of the testicular parenchyma components, total length of the seminiferous tubules, length of the seminiferous tubules per gram of testis, and leydigosomatic and tubulosomatic indexes. The data were evaluated for normality using the Student’s t-test. Data with normal distribution were assessed using analysis of variance (ANOVA) and the non-parametric data were evaluated using the Kruskal-Wallis test, both at 5% probability. Statistically significant differences (p<0.05) were observed for GSI (G1=0.48 ±0.08 and G2=0.34 ±0.09) and HGE (G1=52.95 ±2.99 and G2=.47.63 ±2.67) between treatments. Consumption of C. pyramidale hay increased LW and, consequently, testicular weight, contributing to high GSI. In conclusion, ingestion of C. pyramidale has no toxic effect on the testicular, seminal and histological parameters of goat testis. Due to its nutritional characteristics, consumption of this plant improves animal body development. Because C. pyramidale is adapted to semi-arid regions, it can be an alternative source of feed for goats during periods of shortage.

scholarly journals Efek Serbuk Kunyit dan Kurkumin pada Spermatogenesis Mencit (Mus musculus) yang Diberi Minuman Beralkohol

2021 ◽  
Vol 6 (2) ◽  
pp. 154-160
Author(s):  
Helena Chika Valencia Hanisa ◽  
Tyas Rini Saraswati ◽  
Silvana Tana
Keyword(s):  
Analysis Of Variance ◽  
Mus Musculus ◽  
Alcoholic Beverages ◽  
Seminiferous Tubules ◽  
Treatment Groups ◽  
Alcohol Control ◽  
Randomized Design ◽  
Testicular Weight ◽  
Significant Difference ◽  
Spermatogonia Cells

Kunyit mengandung senyawa kurkumin yang dapat digunakan sebagai zat antiinflamasi dan membantu memperbaiki sel-sel yang rusak. Tujuan dari penelitian ini menganalisis pengaruh serbuk kunyit dan kurkumin pada jumlah dan ukuran sel spermatogonium; spermatosit primer; dan spermatosit sekunder; bobot testis serta diameter tubulus seminiferus Mus musculus yang diberi minuman beralkohol. Penelitian ini merupakan penelitian eksperimental dengan Rancangan Acak Lengkap (RAL), menggunakan 12 ekor Mus musculus jantan yang dibagi kedalam 4 kelompok perlakuan dan 3 kali ulangan. R0 merupakan kontrol, R1 kontrol alkohol, R2 pemberian serbuk kunyit sebanyak 0,1 mg/hari, R3 pemberian kurkumin sebanyak 0,01 mg/hari. Perlakuan diberikan selama 30 hari. Data penelitian dianalisis menggunakan analysis of variance (ANOVA) pada taraf kepercayaan 95%. Berdasarkan hasil penelitian yang diperoleh dapat disimpulkan bahwa tidak terdapat perbedaan bermakna (p>0.05) pada jumlah spermatogonium dan ukuran sel (spermatogonium, spermatosit primer, dan spermatosit sekunder), namun terdapat perbedaan bermakna pada (P<0,05) pada bobot testis, diameter tubulus seminiferus dan jumlah sel (spermatosit primer, dan spermatosit sekunder). Turmeric contains curcumin compounds that can be used as anti-inflammatory substances and help repair damaged cells. The purpose of this study was to analyze the effect of turmeric powder and curcumin on the number and size of spermatogonia cells; primary spermatocytes; and secondary spermatocytes; testicular weight and diameter of the seminiferous tubules of Mus musculus given alcoholic beverages. This study is an experimental study with a completely randomized design (CRD), using 12 male Mus musculus which were divided into 4 treatment groups and 3 replications. R0 is control, R1 is alcohol control, R2 is 0.1 mg/day of turmeric powder, R3 is 0.01 mg/day of curcumin. The treatment was given for 30 days. The research data were analyzed using analysis of variance (ANOVA) at the 95% confidence level. Based on the results obtained, it can be concluded that there is no significant difference (p>0.05) in the number of spermatogonia and cell size (spermatogonia, primary spermatocytes, and secondary spermatocytes), but there is a significant difference (P<0.05) in testicular weight, diameter of the seminiferous tubules and the number of cells (primary spermatocytes, and secondary spermatocytes).

GABAB receptor antagonism from birth to weaning permanently modifies Kiss1 expression in hypothalamus and gonads in mice

2021 ◽  
Author(s):  
Marianne Bizzozzero Hiriart ◽  
Noelia P. Di Giorgio ◽  
Carlos Libertun ◽  
Victoria A.R. Lux-Lantos
Keyword(s):  
Arcuate Nucleus ◽  
Gabab Receptor ◽  
Seminiferous Tubules ◽  
Testis Weight ◽  
Hpg Axis ◽  
Estrous Cycles ◽  
Amino Butyric Acid ◽  
Periventricular Nucleus ◽  
Puberty Onset ◽  
Ovulatory Follicles

Introduction: The kisspeptin gene Kiss1 is expressed in two hypothalamic areas: anteroventral periventricular nucleus/periventricular nucleus (AVPV/PeN) and arcuate nucleus (ARC), and also in gonads. Evidences suggest that gamma-amino butyric acid B receptors (GABAB) signaling can regulate Kiss1 expression. Here we inhibited GABAB signaling from PND2-PND21 and evaluated the hypothalamic-pituitary-gonadal (HPG) axis. Methods: BALB/c mice were treated on postnatal days 2-21 (PND2-PND21) with CGP55845 (GABAB antagonist), and evaluated in PND21 and adulthood: gene expression (qPCR) in hypothalamus and gonads, hormones by radioimmunoassay, gonad histochemistry (H&E), puberty onset, estrous cycles. Results: At PND21, CGP inhibited Kiss1 and Tac2 and increased Pdyn and Gabbr1 in the ARC of both sexes and decreased Th only in female AVPV/PeN. Serum follicle-stimulating hormone (FSH) and testis weight decreased in CGP-males and puberty onset was delayed. In adults, Kiss1, Tac2, Pdyn, Pgr, Cyp19a1, Gad1 were downregulated, while Gabbr1 was upregulated in the ARC of both sexes. In the AVPV/PeN, Kiss1, Th, Cyp19a1 and Pgr decreased while Gad1 increased in CGP-females, whereas Cyp19a1 increased in CGP-males. Serum FSH increased in CGP-males while prolactin increased in CGP-females. Testosterone and progesterone increased in ovaries from CGP-females, in which Kiss1, Cyp19a1 and Esr1 were downregulated while Hsd3b2 was upregulated, together with increased atretic and decreased ovulatory follicles. Testes from CGP-males showed decreased progesterone, increased Gabbr1, Kiss1, Kiss1r, Esr2 and decreased Cyp19a1 and clear signs of seminiferous tubules atrophy. Conclusion: These results demonstrate that appropriate GABAB signaling during this critical prepubertal period is necessary for the normal development of the HPG axis.

Reproductive cycle of purple clam, Amiantis purpurata (Bivalvia: Veneridae) in northern Patagonia (Argentina)

2005 ◽  
Vol 85 (2) ◽  
pp. 367-373 ◽  
Author(s):  
Enrique M. Morsan ◽  
Marina A. Kroeck
Keyword(s):  
Reproductive Cycle ◽  
Continuous Process ◽  
Early Summer ◽  
Maturation Stage ◽  
Reproductive Pattern ◽  
Oocyte Diameter ◽  
Histological Techniques ◽  
Northern Patagonia ◽  
History Of ◽  
Quantitative Indicators

The reproductive cycle of the purple clam (Amiantis purpurata) northern Patagonian population was studied throughout a 19-month period (September 1993–March 1995) using standard histological techniques and quantitative indicators (oocyte diameter distributions, oocytes per ocular field and relative oocyte area). In this species gametogenesis is a continuous process involving a complete gonadal restitution after the end of the reproductive season (no resting period). The proliferation phase occurs during the coldest half of the year (April to September) and maturation takes place during spring (September to December). Several partial emissions of gametes, with subsequent restitution of the gametogenic series, occur during the summer months. During the total maturation stage, oocytes never filled the whole alveolar area: maximum per cent of alveolar sections occupied by oocytes (46%) was observed in late spring–early summer. Implications of the reproductive pattern in the context of palaeogeographic history of northern Patagonia, and the isolation of this population are discussed.

The Influence of Moonlight on the Behavior of Goatsuckers (Caprimulgidae)

The Auk ◽  
1986 ◽  
Vol 103 (2) ◽  
pp. 370-378 ◽  
Author(s):  
Alexander M. Mills
Keyword(s):  
Reproductive Cycle ◽  
Lunar Cycle ◽  
Field Observations ◽  
Nest Activity ◽  
Record Card

Abstract Whip-poor-wills (Caprimulgus vociferus) showed significantly higher levels of locomotory, vocal, and nest activity during twilight and bright moonlight than under moonless conditions. Field observations and nest record card data indicated that Caprimulgus species usually synchronize their reproductive cycle with the lunar cycle. Hatching tends to occur during young waxing moons, presumably so that moonlight-enhanced foraging will be at a maximum while the dependent nestlings are an energy burden on the parents. Observations of foraging Whip-poor-wills suggest they are primarily visually oriented, unlike the bats (Chiroptera).

Relationship between testicular morphology and sperm production following ischaemia in the ram

1995 ◽  
Vol 7 (1) ◽  
pp. 119 ◽  
Author(s):  
CM Markey ◽  
AM Jequier ◽  
GT Meyer ◽  
GB Martin
Keyword(s):  
Morphological Changes ◽  
Sperm Concentration ◽  
Testicular Biopsy ◽  
Seminiferous Tubules ◽  
Testis Weight ◽  
Infertile Men ◽  
Testicular Morphology ◽  
And Function ◽  
The Relationship ◽  
Spermatogenic Epithelium

Arteriosclerosis was induced in the internal spermatic artery of rams to determine if this condition is implicated in the aetiology of testicular pathology which causes male infertility. Data were collected on sperm concentration and motility for 56 days following surgery to provide an index of testicular function. Testes were then weighed and a testicular biopsy score count was performed on histological sections to assess spermatogenic potential of seminiferous tubules. Vascular disturbance caused focal damage of the seminiferous epithelium, similar to that seen among infertile men, and a reduction in ejaculate volume, sperm concentration and sperm motility. Sperm concentration decreased following ischaemia yet was maintained to some degree by a germ-cell depleted spermatogenic epithelium. Normal testicular morphology was maintained above a testis weight of about 120 g (for an individual testis), but below this threshold spermatogenesis was severely impaired. In conclusion, these data have provided information on the relationship between testicular morphology and function following ischaemia in the ram. Furthermore, the morphological changes induced in the testis were similar to those seen among infertile men and, by their focal nature, could explain the distinction between oligozoospermia and azoospermia in men exhibiting spermatogenic arrest.

The reproductive cycle of the viviparous seaperch, Cymatogaster aggregata Gibbons

1968 ◽  
Vol 46 (6) ◽  
pp. 1221-1234 ◽  
Author(s):  
John P. Wiebe
Keyword(s):  
Oocyte Maturation ◽  
Reproductive Cycle ◽  
Sertoli Cells ◽  
Reproductive Behavior ◽  
Seasonal Changes ◽  
Environmental Temperature ◽  
Interstitial Cells ◽  
Early Spring ◽  
Seminiferous Tubules ◽  
Male And Female

The natural reproductive cycle of male and female Cymatogaster aggregata is described with reference to gametogenesis, development of secondary sex structures, reproductive behavior, and gestation. Spermatocytogenesis starts in early spring and by June or July clusters of spermatozoa fill the seminiferous tubules. Concurrently the Sertoli cells and interstitial cells of Leydig increase in size and secondary sex structures develop on the male anal fin. When the sexes mingle in summer, the males perform very elaborate reproductive behavior. Fertilization occurs about mid-December—5 months after mating—and the ovary is then modified to maintain the young embryos until parturition in mid-summer. Oocyte formation is highest in July and August, just after parturition, while vitellogenesis and oocyte maturation occur mainiy from October to December. These seasonal changes are discussed in relation to changes in environmental temperature and photoperiod.

Histologic study of testis injury after bisphenol A exposure in mice

2016 ◽  
Vol 33 (1) ◽  
pp. 36-45 ◽  
Author(s):  
Jijing Tian ◽  
Ye Ding ◽  
Ruiping She ◽  
Longhuan Ma ◽  
Fang Du ◽  
...  
Keyword(s):  
Body Weight ◽  
Bisphenol A ◽  
Tight Junctions ◽  
Basal Lamina ◽  
Cell Injury ◽  
Reproductive Toxicity ◽  
Seminiferous Tubules ◽  
High Dose ◽  
Testis Weight ◽  
Electron Microscopes

The health effects of bisphenol A (BPA) have become a great concern in recent years. In this study, the reproductive toxicity of BPA was investigated. Male CD-1 mice were orally administrated with BPA (0, 100, 300 and 600 mg kg−1 body weight) for 56 consecutive days. Results showed that relative testis weight to total body weight was significantly lower in the high-dose group ( p < 0.01, p < 0.05). Microscopic examination under light and transmission electron microscopes showed disorders of spermatogenesis after BPA exposure, including rough basal lamina of seminiferous tubules and damage of tight junctions between Sertoli cells. Further study by terminal-deoxynucleoitidyl transferase–mediated nick end labelling assay showed a significant induction of apoptosis in the testis tissue of the BPA groups ( p < 0.01). Immunohistochemical study found that the expression of androgen-binding protein (ABP) was significantly decreased in BPA-treated mice ( p < 0.01). Our results indicated that impairment of the basal lamina of seminiferous tubules and tight junctions may contribute to BPA-induced cell injury. A decrease in the level of ABP could be the possible mechanism for the reproductive toxicity of BPA. These findings provided direct evidence and novel insight into the reproductive toxicity of BPA and may have implications for understanding the toxicity of other endocrine disruptors.

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