Behavior of Pseudomonas aeruginosa strains on the nanopillar topography of dragonfly (Pantala flavescens) wing under flow conditions

2021 ◽  
Vol 16 (5) ◽  
pp. 051002
Author(s):  
Banu Pradheepa Kamarajan ◽  
Muthusamy Ananthasubramanian ◽  
Lavanya Sriramajayam ◽  
Appalaraju Boppe
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Flow Conditions ◽  
Pantala Flavescens

Pilot Testing of a Microbiological Method for Disposal of Spent Hydrocarbon Oils

2019 ◽  
Vol 23 (4) ◽  
pp. 25-29
Author(s):  
D.A. Filatov ◽  
V.S. Ovsyannikova ◽  
K.A. Sharshov ◽  
V.A. Zabelin ◽  
D.I. Chuikina
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Enzymatic Activity ◽  
Bacillus Pumilus ◽  
Mineral Medium ◽  
Strain Atcc ◽  
Flow Conditions ◽  
Microbiological Method ◽  
Pilot Testing ◽  
Initial Concentration ◽  
Microbiological Oxidation

Pilot testing of a microbiological method for disposal of spent hydrocarbon oils (HO) by a previously developed consortium of hydrocarbon oxidizing microorganisms (HOM): Aquamicrobium lusatiense strain 854/1 (AM884147); Pseudomonas aeruginosa strain HNYM10 (JN 999888), Paracoccus aminophilus strain ATCC 7061 (NR_043242), Gordonia hydrophobica strain DSM44015 (NR_926254), Bacillus pumilus strain ATCC 7061 (NR_043242). When cultivated under flow conditions in liquid mineral medium with spent oil, their population increased from 1,5–2·104 CFU/ml to 5–6·109 CFU/ml, which was accompanied by a significant increase in their enzymatic activity. The effectiveness of biodegradation of oil at its initial concentration of 25 and 50 wt. % was 93–94 % during 60 days. All hydrocarbons in the composition of the oil to some extent underwent microbiological oxidation, and, depending on the structure, the degree of their disposal was 82–100 %.

scholarly journals Biofilm Formation by Hyperpiliated Mutants of Pseudomonas aeruginosa

2003 ◽  
Vol 185 (7) ◽  
pp. 2374-2378 ◽  
Author(s):  
Poney Chiang ◽  
Lori L. Burrows
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Biofilm Formation ◽  
Growth Conditions ◽  
Twitching Motility ◽  
Wild Type ◽  
Flow Conditions ◽  
Biofilm Structure

ABSTRACT Under static growth conditions, hyperpiliated, nontwitching pilT and pilU mutants of Pseudomonas aeruginosa formed dense biofilms, showing that adhesion, not twitching motility, is necessary for biofilm initiation. Under flow conditions, the pilT mutant formed mushroom-like structures larger than those of the wild type but the pilU mutant was defective in biofilm formation. Therefore, twitching motility affects the development of biofilm structure, possibly through modulation of detachment.

Thrombus formation on artificial surfaces: Correlative microscopy

1990 ◽  
Vol 48 (3) ◽  
pp. 840-841
Author(s):  
Quintin J. Lai ◽  
Stuart L. Cooper ◽  
Ralph M. Albrecht
Keyword(s):  
Electron Microscopy ◽  
High Resolution ◽  
Low Voltage ◽  
Thrombus Formation ◽  
Blood Platelets ◽  
Polymer Surfaces ◽  
Flow Conditions ◽  
Transmission Electron ◽  
Adhesion Of Platelets ◽  
Microscopic Techniques

Thrombus formation and embolization are significant problems for blood-contacting biomedical devices. Two major components of thrombi are blood platelets and the plasma protein, fibrinogen. Previous studies have examined interactions of platelets with polymer surfaces, fibrinogen with platelets, and platelets in suspension with spreading platelets attached to surfaces. Correlative microscopic techniques permit light microscopic observations of labeled living platelets, under static or flow conditions, followed by the observation of identical platelets by electron microscopy. Videoenhanced, differential interference contrast (DIC) light microscopy permits high-resolution, real-time imaging of live platelets and their interactions with surfaces. Interference reflection microscopy (IRM) provides information on the focal adhesion of platelets on surfaces. High voltage, transmission electron microscopy (HVEM) allows observation of platelet cytoskeletal structure of whole mount preparations. Low-voltage, high resolution, scanning electron microscopy allows observation of fine surface detail of platelets. Colloidal gold-labeled fibrinogen, used to identify the Gp Ilb/IIIa membrane receptor for fibrinogen, can be detected in all the above microscopies.

Hydrodynamic flow conditions through permeable walls

1992 ◽  
Vol 2 (8) ◽  
pp. 1565-1569
Author(s):  
S. Vollmar ◽  
J. A. M. S. Duarte
Keyword(s):  
Hydrodynamic Flow ◽  
Flow Conditions ◽  
Permeable Walls
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