scholarly journals Improved recovery of cryopreserved cell monolayers with a hyaluronic acid surface treatment

2020 ◽  
Vol 15 (6) ◽  
pp. 061015
Author(s):  
Giles T. S. Kirby ◽  
Stuart J. Mills ◽  
Tanja E. Mueller ◽  
Anthony E. Ting ◽  
Allison J. Cowin ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Surface Treatment ◽  
Cell Monolayers ◽  
Cryopreserved Cell

SURFACE TREATMENT OF FLEXOR TENDON AUTOGRAFTS WITH CARBODIIMIDE-DERIVATIZED HYALURONIC ACID

2006 ◽  
Vol 88 (10) ◽  
pp. 2181-2191
Author(s):  
CHUNFENG ZHAO ◽  
YU-LONG SUN ◽  
PETER C. AMADIO ◽  
TOSHIKAZU TANAKA ◽  
ANKE M. ETTEMA ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Surface Treatment ◽  
Flexor Tendon

scholarly journals The Effect of Surface Treatment Using Hyaluronic Acid and Lubricin on the Gliding Resistance of Human Extrasynovial Tendons In Vitro

2009 ◽  
Vol 34 (7) ◽  
pp. 1276-1281 ◽  
Author(s):  
Manabu Taguchi ◽  
Chunfeng Zhao ◽  
Yu-Long Sun ◽  
Gregory D. Jay ◽  
Kai-Nan An ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Surface Treatment ◽  
Effect Of Surface

A pre-embedding, protein a-gold immunolabelling technique for cytocentrifuged cell monolayers for lm and tem

1986 ◽  
Vol 44 ◽  
pp. 220-221
Author(s):  
K. Chien ◽  
I.P. Shintaku ◽  
A.F. Sassoon ◽  
R.L. Van de Velde ◽  
R. Heusser
Keyword(s):  
Cell Surface ◽  
Staining Method ◽  
Protein A ◽  
Surface Antigens ◽  
Cell Suspensions ◽  
Cellular Morphology ◽  
Cellular Phenotype ◽  
Cell Surface Antigens ◽  
Cell Monolayers ◽  
Diagnostic Histopathology

Identification of cellular phenotype by cell surface antigens in conjunction with ultrastructural analysis of cellular morphology can be a useful tool in the study of biologic processes as well as in diagnostic histopathology. In this abstract, we describe a simple pre-embedding, protein A-gold staining method which is designed for cell suspensions combining the handling convenience of slide-mounted cell monolayers and the ability to evaluate specimen staining specificity prior to EM embedding.

Processing Immunoperoxidase Labeled Cell Monolayers and Cryostat Sesctions For Electron Microscopy

1985 ◽  
Vol 43 ◽  
pp. 714-715
Author(s):  
K. Chien ◽  
R. Van de Velde ◽  
I.P. Shintaku ◽  
A.F. Sassoon
Keyword(s):  
Cell Monolayer ◽  
Cell Types ◽  
Surface Antigens ◽  
Immunoperoxidase Method ◽  
Reaction Step ◽  
Hot Plate ◽  
Air Drying ◽  
New Approach ◽  
Cell Monolayers ◽  
Glass Slides

Immunoelectron microscopy of neoplastic lymphoma cells is valuable for precise localization of surface antigens and identification of cell types. We have developed a new approach in which the immunohistochemical staining can be evaluated prior to embedding for EM and desired area subsequently selected for ultrathin sectioning.A freshly prepared lymphoma cell suspension is spun onto polylysine hydrobromide- coated glass slides by cytocentrifugation and immediately fixed without air drying in polylysine paraformaldehyde (PLP) fixative. After rinsing in PBS, slides are stained by a 3-step immunoperoxidase method. Cell monolayer is then fixed in buffered 3% glutaraldehyde prior to DAB reaction. After the DAB reaction step, wet monolayers can be examined under LM for presence of brown reaction product and selected monolayers then processed by routine methods for EM and embedded with the Chien Re-embedding Mold. After the polymerization, the epoxy blocks are easily separated from the glass slides by heatingon a 100°C hot plate for 20 seconds.

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