scholarly journals Surface-attached hydrogel coatings via C,H-insertion crosslinking for biomedical and bioanalytical applications (Review)

2018 ◽  
Vol 13 (1) ◽  
pp. 010801 ◽  
Author(s):  
Oswald Prucker ◽  
Thomas Brandstetter ◽  
Jürgen Rühe
Keyword(s):  
Hydrogel Coatings ◽  
Bioanalytical Applications

Controlled synthesis of functional Ag, Ag–Au/Au–Ag nanoparticles and their Prussian blue nanocomposites for bioanalytical applications

RSC Advances ◽  
2015 ◽  
Vol 5 (61) ◽  
pp. 49671-49679 ◽  
Author(s):  
Prem. C. Pandey ◽  
Richa Singh ◽  
Yashashwa Pandey
Keyword(s):  
Prussian Blue ◽  
Ag Nanoparticles ◽  
Active Role ◽  
Reducing Agents ◽  
Controlled Synthesis ◽  
Bioanalytical Applications

A facile method for the synthesis of functional AgNPs and bimetallic Ag–Au/Au–Ag are reported, enabling the formation of nanocomposite with prussian blue in a crystalline framework for bioanalytical applications, showing the active role of organic reducing agents and 3-aminopropyltrimethoxysilane.

scholarly journals Graphene, an Interesting Nanocarbon Allotrope for Biosensing Applications: Advances, Insights, and Prospects

2021 ◽  
Vol 12 ◽  
pp. 117959722098382
Author(s):  
Farid Menaa ◽  
Yazdian Fatemeh ◽  
Sandeep K Vashist ◽  
Haroon Iqbal ◽  
Olga N Sharts ◽  
...  
Keyword(s):  
Cost Effective ◽  
Genetic Alterations ◽  
Multiplex Detection ◽  
Quantitative Characterization ◽  
Disease Biomarkers ◽  
Qualitative And Quantitative ◽  
Sensing Systems ◽  
Doped Graphene ◽  
Bioanalytical Applications

Graphene, a relatively new two-dimensional (2D) nanomaterial, possesses unique structure (e.g. lighter, harder, and more flexible than steel) and tunable physicochemical (e.g. electronical, optical) properties with potentially wide eco-friendly and cost-effective usage in biosensing. Furthermore, graphene-related nanomaterials (e.g. graphene oxide, doped graphene, carbon nanotubes) have inculcated tremendous interest among scientists and industrials for the development of innovative biosensing platforms, such as arrays, sequencers and other nanooptical/biophotonic sensing systems (e.g. FET, FRET, CRET, GERS). Indeed, combinatorial functionalization approaches are constantly improving the overall properties of graphene, such as its sensitivity, stability, specificity, selectivity, and response for potential bioanalytical applications. These include real-time multiplex detection, tracking, qualitative, and quantitative characterization of molecules (i.e. analytes [H2O2, urea, nitrite, ATP or NADH]; ions [Hg2+, Pb2+, or Cu2+]; biomolecules (DNA, iRNA, peptides, proteins, vitamins or glucose; disease biomarkers such as genetic alterations in BRCA1, p53) and cells (cancer cells, stem cells, bacteria, or viruses). However, there is still a paucity of comparative reports that critically evaluate the relative toxicity of carbon nanoallotropes in humans. This manuscript comprehensively reviews the biosensing applications of graphene and its derivatives (i.e. GO and rGO). Prospects and challenges are also introduced.

An engineered lipocalin that tightly complexes the plant poison colchicine for use as antidote and in bioanalytical applications

2019 ◽  
Vol 400 (3) ◽  
pp. 351-366 ◽  
Author(s):  
Mikhail Barkovskiy ◽  
Elena Ilyukhina ◽  
Martin Dauner ◽  
Andreas Eichinger ◽  
Arne Skerra
Keyword(s):  
Protein Design ◽  
Structural Changes ◽  
Peptide Bond ◽  
General Purpose ◽  
Crystallographic Analysis ◽  
Lipocalin 2 ◽  
Intimate Contact ◽  
Poisoning Effect ◽  
Loop Region ◽  
Bioanalytical Applications

Abstract Colchicine is a toxic alkaloid prevalent in autumn crocus (Colchicum autumnale) that binds to tubulin and inhibits polymerization of microtubules. Using combinatorial and rational protein design, we have developed an artificial binding protein based on the human lipocalin 2 that binds colchicine with a dissociation constant of 120 pm, i.e. 10000-fold stronger than tubulin. Crystallographic analysis of the engineered lipocalin, dubbed Colchicalin, revealed major structural changes in the flexible loop region that forms the ligand pocket at the open end of the eight-stranded β-barrel, resulting in a lid-like structure over the deeply buried colchicine. A cis-peptide bond between residues Phe71 and Pro72 in loop #2 constitutes a peculiar feature and allows intimate contact with the tricyclic ligand. Using directed evolution, we achieved an extraordinary dissociation half-life of more than 9 h for the Colchicalin-colchicine complex. Together with the chemical robustness of colchicine and availability of activated derivatives, this also opens applications as a general-purpose affinity reagent, including facile quantification of colchicine in biological samples. Given that engineered lipocalins, also known as Anticalin® proteins, represent a class of clinically validated biopharmaceuticals, Colchicalin may offer a therapeutic antidote to scavenge colchicine and reverse its poisoning effect in situations of acute intoxication.

Synthesis and bioanalytical applications of specific-shaped metallic nanostructures: A review

2012 ◽  
Vol 716 ◽  
pp. 76-91 ◽  
Author(s):  
Shiho Tokonami ◽  
Yojiro Yamamoto ◽  
Hiroshi Shiigi ◽  
Tsutomu Nagaoka
Keyword(s):  
Metallic Nanostructures ◽  
Bioanalytical Applications

ICP-MS as a new tool for the determination of gold nanoparticles in bioanalytical applications

2007 ◽  
Vol 390 (1) ◽  
pp. 249-252 ◽  
Author(s):  
Andy Scheffer ◽  
Carsten Engelhard ◽  
Michael Sperling ◽  
Wolfgang Buscher
Keyword(s):  
Gold Nanoparticles ◽  
Icp Ms ◽  
Bioanalytical Applications
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