Six species in one: evidence of cryptic speciation in the Hygrobates fluviatilis complex (Acariformes, Hydrachnidia, Hygrobatidae)

2017 ◽  
Vol 22 (9) ◽  
pp. 1327 ◽  
Author(s):  
Vladimir Pešić ◽  
Mahdieh Asadi ◽  
Mirela Cimpean ◽  
Miroslawa Dabert ◽  
Yunus Esen ◽  
...  
Keyword(s):  
Statistical Analysis ◽  
Morphological Characters ◽  
Morphological Data ◽  
Cryptic Speciation ◽  
Dna Barcodes ◽  
Water Mites ◽  
Additional Species ◽  
Biogeographic Regions ◽  
Coi Sequences ◽  
The Antarctic

Water mites of the genus Hygrobates are widely distributed in all biogeographic regions except the Antarctic. Palaearctic Hygrobates species with reticulated soft integument generally have been considered as representatives of one common and widely distributed species, Hygrobates fluviatilis Strøm, 1768. Based on partial COI sequences (DNA-barcodes) and statistical analysis of morphological data, we show that these mites belong to six distinct lineages. Two of them are widely distributed in Central Europe: Hygrobates fluviatilis here redescribed based on a neotype designated from the type locality in Norway, and a species new to science, H. arenarius Smit & Pešić. The four remaining lineages represent additional species new to science that appear to have more restricted distributions: H. corsicus Pešić & Smit (Corsica, Sardinia), H. marezaensis Pešić & Dabert (Montenegro, Albania, Croatia), H. turcicus Pešić, Esen & Dabert (Turkey), and H. persicus Pešić & Asadi (Iran, E Turkey). Statistical morphometric analysis reveals that the latter two species cannot be separated on morphological characters and should be considered true cryptic species. We provide data concerning biology and geographical distributions together with a key to all species of the complex.

A new skink of the genus Scincella Mittleman, 1950 (Squamata: Scincidae) from Ba Den Mountain, Tay Ninh Province, southern Vietnam

Zootaxa ◽  
2019 ◽  
Vol 4648 (2) ◽  
pp. 273-286
Author(s):  
SANG NGOC NGUYEN ◽  
VU DANG HOANG NGUYEN ◽  
LUAN THANH NGUYEN ◽  
ROBERT W. MURPHY
Keyword(s):  
New Species ◽  
Endemic Species ◽  
Morphological Characters ◽  
Nucleotide Sequences ◽  
Morphological Data ◽  
Medium Size ◽  
Southern Vietnam ◽  
Males And Females ◽  
Coi Sequences ◽  
Dorsal Pattern

Ba Den is an isolated mountain in southern Vietnam and home to two endemic species of lizards. Herein, we describe another endemic species, a new skink of the genus Scincella Mittleman, 1950, from the area based on morphological data, including hemipenial characters and nucleotide sequences of COI. The following morphological characters diagnose Scincella badenensis sp. nov.: medium size in adults (snout-vent length up to 64.4 mm); toes reach to fingers when limbs adpressed; midbody scale rows 32–36, smooth; paravertebral scales 67–71; dorsal scales not enlarged; ventral scale rows 68–74; supraoculars four; prefrontals in broad contact with one another; loreal scales two; tympanum deeply sunk, without auricular lobules; two enlarged anterior temporal scales; smooth lamellae beneath toe IV 18–20; pair of enlarged precloacal scales; hemipenes short, smooth and forked near the tip with two short lobes and two small terminal papillae; no dorsal pattern in males; and females with black interruptive vertebral line. The new species differs from its congeners by at least 10.4% uncorrected p-distance in COI sequences. 

scholarly journals Taxonomic Position of Anastrangalia reyi and A. sequensi (Coleoptera, Cerambycidae) Based on Molecular and Morphological Data

2019 ◽  
Vol 53 (3) ◽  
pp. 209-226 ◽  
Author(s):  
A. M. Zamoroka ◽  
D. V. Semaniuk ◽  
V. Yu. Shparyk ◽  
T.V. Mykytyn ◽  
S. V. Skrypnyk
Keyword(s):  
Male Genitalia ◽  
Meta Analysis ◽  
Introgressive Hybridization ◽  
Morphological Characters ◽  
Taxonomic Position ◽  
Morphological Data ◽  
Similar Species ◽  
Dense Cluster ◽  
Coi Sequences ◽  
Xix Century

Abstract Anastrangalia reyi (Heyden, 1889) and Anastrangalia sequensi (Reitter, 1898) are morphologically similar species described in late of XIX century. The recent barcoding revealed that A. reyi is almost identical to another species, Anastrangalia dubia (Scopoli, 1763), by the sequence of nucleotides in cytochrome C oxidase subunit I (COI). Consequently, the taxonomic position of these species is unclear. We have conducted a comprehensive meta-analysis of available data of COI sequences combined with a study of morphological characters of the male genitalia of A. reyi, A. sequensi and A. dubia. Based on 87 sequenced samples we built well-resolved phylogenetic maximum likelihood tree. We found the clades of A. dubia, A. reyi and A. sequensi to be closely related and arranged in the dense cluster. Despite this, numerous cases of introgressive hybridization of A. reyi and A. dubia were identified, indicating an inadequate reproductive barrier between them. The study of morphological features of male genitalia of A. reyi, A. sequensi and A. dubia shows minor differences between them. Based on these facts and the results of the phylogenetic analysis we propose to consider A. reyi and A. sequensi to be subspecies of A. dubia.

scholarly journals Integrative taxonomy supports the presence of two species of Kyphosus (Perciformes: Kyphosidae) in Atlantic European waters

2017 ◽  
Vol 81 (4) ◽  
pp. 467 ◽  
Author(s):  
Rafael Bañón ◽  
David Barros-García ◽  
Alejandro De Carlos
Keyword(s):  
New Record ◽  
Morphological Characters ◽  
Sympatric Species ◽  
Integrative Taxonomy ◽  
Index Number ◽  
Dna Barcodes ◽  
Northwestern Spain ◽  
Kyphosus Vaigiensis ◽  
Coi Sequences ◽  
Public Repositories

The taxonomic identification of one Kyphosus sectatrix and two Kyphosus vaigiensis specimens caught in the European Atlantic waters of Galicia, northwestern Spain, was carried out by means of integrative taxonomy, combining the examination of morphological characters and DNA barcodes. Taxonomical assignation based on morphological characters of these specimens was tested by comparing their COI sequences with available data of Kyphosus deposited in public repositories. The resulting neighbour-joining tree defined four clades corresponding to Barcode Index Number (BIN) and indicated that some nucleotide sequences from Kyphosus, previously deposited, probably originate from misidentified specimens, as would be expected from cryptic and sympatric species. The specimens of Kyphosus vaigiensis represent a new record for the waters of Galicia and the northernmost record in the eastern Atlantic. This kind of herbivorous tropical fishes could play an important role in the tropho-dynamic context of this temperate coastal ecosystem.

scholarly journals New records of water mites from the Balkans revealed by DNA barcoding (Acari, Hydrachnidia)

2021 ◽  
Vol 49 ◽  
pp. 20-34
Author(s):  
Vladimir Pešić ◽  
Milica Jovanović ◽  
Ana Manović ◽  
Ioannis Karaouzas ◽  
Harry Smit
Keyword(s):  
Dna Barcoding ◽  
New Records ◽  
Cryptic Diversity ◽  
Dna Barcodes ◽  
Water Mites ◽  
Water Mite ◽  
The Balkans ◽  
Coi Sequences ◽  
First Time

New records of water mites from Serbia, North Macedonia and Greece revealed by DNA barcoding are presented. DNA barcodes were recovered from 63 water mite specimens morphologically assigned to 32 species. One species Lebertia algeriensis Lundblad, 1942, is reported for the first time for the Balkans; four species, i.e. Lebertia fimbriata Thor, 1899, L. rivulorum K. Viets, 1933, Atractides stankovici Pešić & Gerecke, 2010, and Wettina lacustris Pešić & Smit, 2018 are new for North Macedonia; Monatractides madritensis (K. Viets, 1930) and Mideopsis roztoczensis Biesiadka & Kowalik, 1979 are new for Serbia; and Hygrobates mediterraneus Pešić, 2020 is new for Greece. Comparing sequences of our study with the available COI sequences we recognized five possible cases of cryptic diversity involving species clusters with more than 5% divergence indicating that these species need further study.

Redescription and neotype designation of the Antarctic springtail Folsomotoma octooculata (Collembola: Isotomidae)

Zootaxa ◽  
2018 ◽  
Vol 4392 (2) ◽  
pp. 392
Author(s):  
PIETRO PAOLO FANCIULLI ◽  
CHIARA LEO ◽  
PETER CONVEY ◽  
FRANCESCO FRATI ◽  
ANTONIO CARAPELLI
Keyword(s):  
South America ◽  
Morphological Characters ◽  
Morphological Data ◽  
Collembolan Species ◽  
Molecular Aspects ◽  
The Antarctic ◽  
Antarctic Environment

The growing interest in Antarctic collembolan species has led us to re-evaluate both the morphological and molecular aspects of different species adapted to the extreme Antarctic environment. The genus Folsomotoma includes nine species mainly distributed in Antarctic and subantarctic areas and a few additionally from Australia and South America. We herein designate the neotype of Folsomotoma octooculata (Willem 1901) and redescribe its morphological characters with particular emphasis on the sensory and ordinary chaetotaxy. Furthermore, based on analyses of both our new and previously published morphological data for F. octooculata, we propose a systematic key of the species for the genus. 

Uncovering the hidden biodiversity of natural history collections: Insights from DNA barcoding and morphological characters of the Neotropical genus Orthocomotis Dognin (Lepidoptera: Tortricidae)

Zootaxa ◽  
2017 ◽  
Vol 4250 (6) ◽  
pp. 541 ◽  
Author(s):  
JÓZEF RAZOWSKI ◽  
VOLKER PELZ ◽  
SEBASTIAN TARCZ
Keyword(s):  
Natural History ◽  
Host Plants ◽  
Mitochondrial Gene ◽  
Dna Barcode ◽  
Molecular Study ◽  
Morphological Characters ◽  
Morphological Data ◽  
Rapid Speciation ◽  
Natural History Collections ◽  
Coi Sequences

We used a 227-bp fragment of the mitochondrial gene cytochrome oxidase I (DNA “barcode”) in conjunction with morphological data to study specimens of the Neotropical genus Orthocomotis Dognin, 1906, acquired from natural history collections. We examined over 20 species of Orthocomotis from 17 localities in Colombia, Ecuador, and Peru. The analysis identified 32 haplotypes among the 62 specimens and found no haplotypes shared among species. The molecular study revealed not only the usefulness of short COI sequences in discriminating among Orthocomotis species but also showed distinctness of four clusters which correspond to those based on morphological (genitalia) characters. Moreover, the molecular results suggest the occurrence of rapid speciation in Orthocomotis. We hypothesize that this may be linked to the great biodiversity of potential host plants in Neotropical ecosystems. 

scholarly journals Phylogeny and systematics of the leafhopper subfamily Ledrinae (Hemiptera: Cicadellidae)

Zootaxa ◽  
2009 ◽  
Vol 2186 (1) ◽  
pp. 1-120 ◽  
Author(s):  
JOSHUA R. JONES ◽  
LEWIS L. DEITZ
Keyword(s):  
Morphological Characters ◽  
Morphological Data ◽  
New Combinations ◽  
Additional Species ◽  
Very Old ◽  
Phylogenetic Framework ◽  
Phylogenetic Hypotheses ◽  
Five Tribes

Ledrinae represent a relatively small subfamily within the very large and diverse leafhopper family Cicadellidae. Prior to this study, the subfamily contained approximately 450 species in 5 tribes and 73 genera distributed worldwide, but primarily in Australia, Africa, and the Indomalayan region. Several authors have suggested Ledrinae are not monophyletic, and recent studies based on molecular and morphological data have called the existing classification into question. The goals of this study were to collect and analyze morphological data under rigorous phylogenetic criteria in order to test the monophyly of Ledrinae, provide robust definitions for the subfamily and its tribes, and provide a phylogenetic framework for understanding relationships among these leafhoppers. New phylogenetic hypotheses are presented here regarding the monophyly of Ledrinae and its included tribes. A total of 235 morphological characters were coded for 60 species from 31 genera in the tribes Ledrini and Petalocephalini, 5 species from the tribes Stenocotini, Thymbrini, and Xerophloeini, and 10 species selected as outgroups from various other cicadellid subfamilies. Data were analyzed in PAUP*, resulting in a single completely resolved topology with many well-supported nodes. Ledrinae, as previously defined, were found to be polyphyletic with respect to Stenocotini, Thymbrini, and various ledrine genera, which were placed with the outgroup taxa. Stenocotini and Thymbrini were placed with Tartessinae, agreeing with results of other recent studies. Rubria, Hespenedra, and the Afrorubria genus group formed independent lineages within Ledrinae. The tribe Xerophloeini was placed as a basal lineage within Ledrinae closely associated with the Afrorubria group. Ledrini and Petalocephalini, as previously defined, did not form monophyletic lineages, but were randomly interspersed (paraphyletic) with respect to one another. Stenocotini and Thymbrini are here removed to the subfamily Tartessinae, and Petalocephalini is made a synonym of Ledrini. The new tribes Afrorubrini, Hespenedrini, and Rubrini are described within Ledrinae by the first author, and several new informal genus groups are named. Within Ledrini, Epiclinata Metcalf is made a synonym of Tituria Stål, Epiclinata planata (Fabricius) and Epiclinata flavomarginata Kuoh & Cai are moved to Tituria, Tituria obtusa Walker is moved to Thlasia Germar, and two additional species are included in Latycephala McKamey as new combinations. Taxa placed outside of Ledrinae in this analysis are removed to other subfamilies or considered unplaced within Cicadellidae. This newly revised classification strengthens hypotheses that the Ledrinae are a very old group within Cicadellidae. As here defined, the subfamily includes five tribes with a total of 38 genera and approximately 300 species.

Annotated and updated checklist of marine crabs (Decapoda: Brachyura) of Mozambique supported by morphological and molecular data from shelf and slope species of the “MOZAMBIQUE” surveys

Zootaxa ◽  
2021 ◽  
Vol 5056 (1) ◽  
pp. 1-67
Author(s):  
ISABEL MUÑOZ ◽  
EVA GARCÍA-ISARCH ◽  
JOSE A. CUESTA
Keyword(s):  
Dna Barcode ◽  
Morphological Characteristics ◽  
Morphological Characters ◽  
Molecular Data ◽  
Morphological Data ◽  
Colour Pattern ◽  
General Distribution ◽  
Bathymetric Range ◽  
Coi Sequences ◽  
First Time

An updated checklist of Mozambican marine brachyuran crabs is generated based on an exhaustive revision of the existing literature, together with the additional records provided by the specimens collected throughout the three “MOZAMBIQUE” surveys carried out in Mozambican waters during three consecutive years (2007–2009) by the Instituto Español de Oceanografía, (Spanish Institute of Oceanography, IEO). A total of 269 species, grouped in 15 superfamilies, 26 families and 172 genera are reported in the checklist, and a detailed inventory is produced with the list and remarks about the brachyuran species collected. Thirty-nine crab species belonging to 19 families were identified based on morphological characteristics and/or genetic tools. DNA barcode sequences (16S rRNA and/or COI) were obtained for 37 species, including 16S and COI sequences that are new for 26 and 14 species, respectively. Colour photographs of fresh specimens illustrate the comments about most species, being the first time that the original colour pattern is described for some of them. New records in Mozambican waters are reported for the species Paromolopsis boasi, Mursia aspera, Carcinoplax ischurodous, Tanaoa pustulosus, Euclosiana exquisita, Oxypleurodon difficilis, Naxioides robillardi, Samadinia galathea, Cyrtomaia gaillardi, Paramaja gibba, Pleistacantha ori, Parathranites granosus, Parathranites orientalis, Ovalipes iridescens and Charybdis smithii, and second records for Moloha alcocki, Samadinia pulchra and Charybdis africana. In addition, Raninoides crosnieri, S. galathea and P. ori were collected for the first time after their descriptions. The female of Samadinia galathea is described for the first time, and a potential new species of Mursia is reported. Some records expand the known bathymetric range of certain species and/or their general distribution. New molecular and morphological data suggest the necessity of the revision of P. boasi, R. crosnieri, C. africana and the genera Platymaia and Carcinoplax. The variability and taxonomic validity of some morphological characters in brachyuran systematic is discussed.  

Morphology and DNA barcodes of two species of Bradysia Winnertz from China (Diptera, Sciaridae), with the description of Bradysia minorlobus Yang, Shi & Huang sp. n.

Zootaxa ◽  
2019 ◽  
Vol 4612 (1) ◽  
pp. 85 ◽  
Author(s):  
XUE YANG ◽  
KAI SHI ◽  
KAI HELLER ◽  
FRANK MENZEL ◽  
JUNHAO HUANG ◽  
...  
Keyword(s):  
Morphological Characters ◽  
Species Group ◽  
Genetic Distances ◽  
Species Concepts ◽  
Similar Species ◽  
Dna Barcodes ◽  
Neighbor Joining ◽  
Coi Sequences ◽  
Morphological Species ◽  
Group Species

Two morphologically similar species of the fungicola species group of Bradysia Winnertz, 1867 were studied in China: Bradysia chenjinae Yang, Zhang & Yang, 1993 and Bradysia minorlobus Yang, Shi & Huang sp. n. The morphological species concepts were supported by the DNA barcodes of COI sequences. The genetic distances of 16 Bradysia fungicola group species were analyzed and a neighbor-joining tree was constructed. The morphological characters of both Chinese species were described and illustrated. 

A new skink of the genus Scincella Mittleman, 1950 (Squamata: Scincidae) from southern Vietnam

Zootaxa ◽  
2020 ◽  
Vol 4868 (3) ◽  
pp. 423-434
Author(s):  
SANG NGOC NGUYEN ◽  
VU DANG HOANG NGUYEN ◽  
LUAN THANH NGUYEN ◽  
ROBERT W. MURPHY
Keyword(s):  
New Species ◽  
Morphological Characters ◽  
Nucleotide Sequences ◽  
Morphological Data ◽  
Temporal Scales ◽  
Southern Vietnam ◽  
Coi Sequences

A new skink of the genus Scincella Mittleman, 1950, from Binh Phuoc Province, southern Vietnam is described based on morphological data, including hemipenial characters, and nucleotide sequences from COI. The following morphological characters diagnose Scincella baraensis sp. nov.: relatively small size in adults (snout-vent length up to 49.2 mm); when limbs adpressed, toes do not or just reach fingers; 30 smooth midbody scale rows and dorsal scales not enlarged; 66–70 paravertebral scales; 64–66 ventral scale rows; 7 supralabials, the fifth and sixth below the center of the eye; enlarged temporal scales 1+2; nuchals present; ear with small auricular lobules; 18–20 smooth lamellae beneath toe IV; hemipenis smooth, short, and unforked; dorsum with faint black dots. The new species differs from its congeners by an uncorrected p-distance in COI sequences of at least 16.2%. 

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