Tetranychus urticae (green form) on Gossypium hirsutum in China: two records confirmed by aedeagus morphology and RFLP analysis

2013 ◽  
Vol 18 (3) ◽  
pp. 239 ◽  
Author(s):  
CHENG GE ◽  
XIU-LEI DING ◽  
JIAN-PING ZHANG ◽  
XIAO-YUE HONG
Keyword(s):  
Gossypium Hirsutum ◽  
Tetranychus Urticae ◽  
Rflp Analysis ◽  
Green Form

Molecular identification of fungi isolated from stem tissue of Upland cotton (Gossypium hirsutum)

2005 ◽  
Vol 53 (6) ◽  
pp. 571 ◽  
Author(s):  
L. Augusto Becerra-LopezLavalle ◽  
Jennifer A. Saleeba ◽  
Bruce R. Lyon
Keyword(s):  
Gossypium Hirsutum ◽  
Restriction Analysis ◽  
Rflp Analysis ◽  
Molecular Techniques ◽  
Internal Transcribed Spacers ◽  
Pcr Analysis ◽  
Rrna Gene ◽  
5.8S Rrna Gene ◽  
Rdna Sequences ◽  
Rdna Sequencing

Molecular techniques such as restriction fragment length polymorphism (RFLP) analysis, random amplification of polymorphic DNA (RAPD) fingerprinting, and DNA sequencing and database comparison, were employed to identify fungi isolated from field-grown cotton plants (Gossypium hirsutum L.). DNA fragments of between 510 and 590 bp, representing the two rDNA (rDNA) internal transcribed spacers (ITS1 and ITS2) and the intervening 5.8S rRNA gene, were amplified from the fungi with eukaryotic consensus primers. Subsequent digestion with the restriction endonucleases AluI, CfoI, HaeIII, HinfI and HpaII enabled the allotment of all 57 isolates to 13 different groups. Restriction analysis was supported by RAPD–PCR analysis of multiple isolates and rDNA sequencing of representative fungi from each group. Sequence alignment and comparison with rDNA sequences of other fungi available in GenBank allowed for putative identification of three different taxa of Fusarium, two taxa each of Cladosporium, Diaporthe and Nectria, and one taxon each of Alternaria, Ampelomyces, Bartalinia, Phaeosphaeria and Rhizoctonia. Many of the stem-colonising fungi identified in this study are either pathogenic on cotton or have elsewhere been found to act as biocontrol agents.

scholarly journals Effects of Acaricides on Tetranychus urticae Koch (Green Form) collected from strawberry greenhouses in Okayama Prefecture, Japan

2021 ◽  
Vol 63 (0) ◽  
pp. 21-25
Author(s):  
Yusuke Nishi ◽  
Shigeyuki Nagamori ◽  
Toshihiro Sano ◽  
Kana Namba ◽  
Hiroyuki Matsuoka
Keyword(s):  
Tetranychus Urticae ◽  
Green Form ◽  
Okayama Prefecture

scholarly journals Efeitos de subprodutos da fotodegradação da deltametrina na população de Tetranychus urticae Koch (Acari: Tetranychidae)

1999 ◽  
Vol 28 (3) ◽  
pp. 511-517
Author(s):  
Maria L.B. Trindade ◽  
Luiz G. Chiavegato
Keyword(s):  
Gossypium Hirsutum ◽  
Tetranychus Urticae ◽  
Gossypium Hirsutum L ◽  
Tetranychus Urticae Koch

O objetivo deste trabalho foi avaliar os efeitos da aplicação em algodoeiros (Gossypium hirsutum L.), do ácido-fenoxibenzóico (principal subproduto da fotodegradação da deltametrina e de muitos outros piretróides) e de emulsões aquosas de Deltametrina (expostas à luz solar por dois, oito e 15 dias antes da pulverização) sobre a população do ácaro Tetranychus urticae Koch. Nas plantas pulverizadas com emulsões de Deltametrina expostas à luz solar e com ácido fenoxi-benzóico (nas maiores doses) as populações de T. urticae foram significativamente maiores que nas plantas testemunhas.

Molecular authentication of Thai medicinal plant Khamin khruea by PCR-RFLP analysis

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
P Rojsanga ◽  
W Gritsanapan ◽  
W Leelamanit ◽  
S Sukrong
Keyword(s):  
Medicinal Plant ◽  
Rflp Analysis ◽  
Molecular Authentication ◽  
Pcr Rflp
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