Quantitative Mapping of Local Mechanical Properties of Living Cells at Near-Physiological Conditions Using Multi-Harmonic Atomic Force Microscopy

2012 ◽  
Author(s):  
Alexander Cartagena ◽  
Wen-Horng Wang ◽  
Robert L. Geahlen ◽  
Arvind Raman
Keyword(s):  
Atomic Force Microscopy ◽  
Living Cells ◽  
Cellular Biology ◽  
Force Microscopy ◽  
Nanomechanical Properties ◽  
Physiological Conditions ◽  
Atomic Force ◽  
Quantitative Mapping ◽  
Force Volume ◽  
Force Displacement

Measurements of local nanomechanical properties of living cells recently have become extremely important for the cellular biology and biomechanics communities [1]. The measurement of progressive variations in viscoelastic properties of living cells in their native physiological liquid environments can provide significant insight to the mechanistic processes underpinning morphogenesis, mechano-transduction, motility, metastasis, aging, etc. Atomic Force Microscopy (AFM) based biomechanical assays have the unique advantage of resolving/mapping spatially the local nanomechanical properties over the cell surface. However current methods using standard force-volume, force displacement curves [2–3] are very low resolution and low speed making them completely incompatible for biomechanical assays of living cells.

scholarly journals Nanomechanics in Monitoring the Effectiveness of Drugs Targeting the Cancer Cell Cytoskeleton

2020 ◽  
Vol 21 (22) ◽  
pp. 8786
Author(s):  
Andrzej Kubiak ◽  
Tomasz Zieliński ◽  
Joanna Pabijan ◽  
Małgorzata Lekka
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Death ◽  
Actin Filaments ◽  
High Spatial Resolution ◽  
Living Cells ◽  
Antitumor Drugs ◽  
Antitumor Therapy ◽  
Force Microscopy ◽  
Nanomechanical Properties ◽  
Atomic Force

Increasing attention is devoted to the use of nanomechanics as a marker of various pathologies. Atomic force microscopy (AFM) is one of the techniques that could be applied to quantify the nanomechanical properties of living cells with a high spatial resolution. Thus, AFM offers the possibility to trace changes in the reorganization of the cytoskeleton in living cells. Impairments in the structure, organization, and functioning of two main cytoskeletal components, namely, actin filaments and microtubules, cause severe effects, leading to cell death. That is why these cytoskeletal components are targets for antitumor therapy. This review intends to describe the gathered knowledge on the capability of AFM to trace the alterations in the nanomechanical properties of living cells induced by the action of antitumor drugs that could translate into their effectiveness.

scholarly journals Cell penetration efficiency analysis of different atomic force microscopy nanoneedles into living cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Marcos Penedo ◽  
Tetsuya Shirokawa ◽  
Mohammad Shahidul Alam ◽  
Keisuke Miyazawa ◽  
Takehiko Ichikawa ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Membrane ◽  
Cell Biology ◽  
Cell Damage ◽  
Efficiency Analysis ◽  
Living Cells ◽  
Biomolecular Recognition ◽  
Cell Penetration ◽  
Force Microscopy ◽  
Atomic Force

AbstractOver the last decade, nanoneedle-based systems have demonstrated to be extremely useful in cell biology. They can be used as nanotools for drug delivery, biosensing or biomolecular recognition inside cells; or they can be employed to select and sort in parallel a large number of living cells. When using these nanoprobes, the most important requirement is to minimize the cell damage, reducing the forces and indentation lengths needed to penetrate the cell membrane. This is normally achieved by reducing the diameter of the nanoneedles. However, several studies have shown that nanoneedles with a flat tip display lower penetration forces and indentation lengths. In this work, we have tested different nanoneedle shapes and diameters to reduce the force and the indentation length needed to penetrate the cell membrane, demonstrating that ultra-thin and sharp nanoprobes can further reduce them, consequently minimizing the cell damage.

scholarly journals Anisotropic Mechanical Properties of Living Cells Revealed by Integrated Spinning Disk Confocal and Atomic Force Microscopy

2018 ◽  
Vol 114 (3) ◽  
pp. 513a
Author(s):  
Yuri M. Efremov ◽  
Mirian Velay-Lizancos ◽  
Daniel M. Suter ◽  
Pablo D. Zavattieri ◽  
Arvind Raman
Keyword(s):  
Mechanical Properties ◽  
Atomic Force Microscopy ◽  
Living Cells ◽  
Force Microscopy ◽  
Atomic Force ◽  
Spinning Disk ◽  
Anisotropic Mechanical Properties

scholarly journals 3P-311 Viscoelastic Properties of Many Living Cells Investigated by Atomic Force Microscopy(The 46th Annual Meeting of the Biophysical Society of Japan)

2008 ◽  
Vol 48 (supplement) ◽  
pp. S175
Author(s):  
Shinichiro Hiratsuka ◽  
Yusuke Mizutani ◽  
Masahiro Tsuchiya ◽  
Koichi Kawahara ◽  
Hiroshi Tokumoto ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Annual Meeting ◽  
Viscoelastic Properties ◽  
Living Cells ◽  
Force Microscopy ◽  
Atomic Force ◽  
Biophysical Society

Nanomechanical properties of lithiated Si nanowires probed with atomic force microscopy

2012 ◽  
Vol 45 (27) ◽  
pp. 275301 ◽  
Author(s):  
Hyunsoo Lee ◽  
Weonho Shin ◽  
Jang Wook Choi ◽  
Jeong Young Park
Keyword(s):  
Atomic Force Microscopy ◽  
Si Nanowires ◽  
Force Microscopy ◽  
Nanomechanical Properties ◽  
Atomic Force

scholarly journals Continuous detection of extracellular ATP on living cells by using atomic force microscopy

1999 ◽  
Vol 96 (21) ◽  
pp. 12180-12185 ◽  
Author(s):  
S. W. Schneider ◽  
M. E. Egan ◽  
B. P. Jena ◽  
W. B. Guggino ◽  
H. Oberleithner ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Living Cells ◽  
Extracellular Atp ◽  
Force Microscopy ◽  
Atomic Force
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