Dependence of Cyclic Stretch-Induced Stress Fiber Reorientation on Stretch Waveform

2012 ◽  
Author(s):  
Abhishek Tondon ◽  
Hui-Ju Hsu ◽  
Roland Kaunas
Keyword(s):  
Stress Fiber ◽  
Cyclic Stretch ◽  
Induced Stress ◽  
Fiber Reorientation

Dependence of cyclic stretch-induced stress fiber reorientation on stretch waveform

2012 ◽  
Vol 45 (5) ◽  
pp. 728-735 ◽  
Author(s):  
Abhishek Tondon ◽  
Hui-Ju Hsu ◽  
Roland Kaunas
Keyword(s):  
Stress Fiber ◽  
Cyclic Stretch ◽  
Induced Stress ◽  
Fiber Reorientation

J028035 Mechanism of strain waveform-dependent stress fiber reorientation in MC3T3-E1 cells induced with cyclic stretch

2012 ◽  
Vol 2012 (0) ◽  
pp. _J028035-1-_J028035-5
Author(s):  
Kazuaki NAGAYAMA ◽  
Yuki KIMURA ◽  
Narutaka MAKINO ◽  
Takeo MATSUMOTO
Keyword(s):  
Stress Fiber ◽  
Cyclic Stretch ◽  
Fiber Reorientation

scholarly journals Stress fiber growth and remodeling determines cellular morphomechanics under uniaxial cyclic stretch

2019 ◽  
Author(s):  
Aritra Chatterjee ◽  
Paturu Kondaiah ◽  
Namrata Gundiah
Keyword(s):  
Effective Elastic Modulus ◽  
Stress Fiber ◽  
Cyclic Stretch ◽  
Stress Fibers ◽  
Avoidance Reaction ◽  
Cortical Actin ◽  
Fiber Growth ◽  
Induced Stress ◽  
Reorientation Dynamics ◽  
And Migration

AbstractStress fibers in the cytoskeleton are essential in maintaining cellular shape, and influence their adhesion and migration. Cyclic uniaxial stretching results in cellular reorientation orthogonal to the applied stretch direction via a strain avoidance reaction; the mechanistic cues in cellular mechanosensitivity to this response are currently underexplored. We show stretch induced stress fiber lengthening, their realignment and increased cortical actin in fibroblasts stretched over varied amplitudes and durations. Higher amounts of actin and alignment of stress fibers were accompanied with an increase in the effective elastic modulus of cells. Microtubules did not contribute to the measured stiffness or reorientation response but were essential to the nuclear reorientation. We modeled stress fiber growth and reorientation dynamics using a nonlinear, orthotropic, fiber-reinforced continuum representation of the cell. The model predicts the observed fibroblast morphology and increased cellular stiffness under uniaxial cyclic stretch. These studies are important in exploring the differences underlying mechanotransduction and cellular contractility under stretch.

The Direction of Cyclic Stretch-Induced Stress Fiber Orientation Depends on Matrix Rigidity

2011 ◽  
Author(s):  
Abhishek Tondon ◽  
Hui-Ju Hsu ◽  
Roland Kaunas
Keyword(s):  
Theoretical Model ◽  
Fiber Orientation ◽  
Stress Fiber ◽  
Cyclic Stretch ◽  
Matrix Rigidity ◽  
Structural Element ◽  
Cellular Processes ◽  
Induced Stress ◽  
Cellular Environment ◽  
Collagen Hydrogels

Mechanical properties of the cellular environment such as elastic rigidity have been shown to play an important role in the regulation of important cellular processes such as proliferation, differentiation and apoptosis (1–3). Intracellular tension decreases with decreasing matrix rigidity (1). Actin stress fibers (SFs), the major structural element in cells bearing tension, are also less prevalent on soft vs. rigid matrices (4). We have developed a theoretical model of stretch-induced SFs that predicts SFs reorient perpendicular to the direction of cyclic stretch in order to maintain SF tension at a homeostatic level (5). A theoretical model developed by the Safran group (6) predicts that cells will also align perpendicular to cyclic stretch on soft substrates. To test these predictions, we subjected cells to cyclic uniaxial stretch on soft collagen hydrogels. Interestingly, the cells and their SFs aligned parallel to the direction of stretch without co-alignment of collagen fibrils, indicating the need for a new model to describe the effects of cyclic stretch on SF reorganization on soft matrices.

Synergy between Rho signaling and matrix density in cyclic stretch-induced stress fiber organization

2014 ◽  
Vol 10 (5) ◽  
pp. 1876-1885 ◽  
Author(s):  
Jasper Foolen ◽  
Marloes W.J.T. Janssen-van den Broek ◽  
Frank P.T. Baaijens
Keyword(s):  
Stress Fiber ◽  
Cyclic Stretch ◽  
Matrix Density ◽  
Induced Stress ◽  
Fiber Organization

Cyclic stretch-induced stress fiber dynamics – Dependence on strain rate, Rho-kinase and MLCK

2010 ◽  
Vol 401 (3) ◽  
pp. 344-349 ◽  
Author(s):  
Chin-Fu Lee ◽  
Candice Haase ◽  
Shinji Deguchi ◽  
Roland Kaunas
Keyword(s):  
Strain Rate ◽  
Rho Kinase ◽  
Stress Fiber ◽  
Cyclic Stretch ◽  
Induced Stress ◽  
Fiber Dynamics

Mechanically Optimized Orientation of Intracellular Stress Fibers Under Cyclic Stretch

2000 ◽  
Author(s):  
Hiroshi Yamada ◽  
Tohru Takemasa ◽  
Takami Yamaguchi
Keyword(s):  
Endothelial Cell ◽  
Continuum Mechanics ◽  
Cellular Response ◽  
Length Change ◽  
Mechanical Stimulus ◽  
Stress Fiber ◽  
Cyclic Stretch ◽  
Stress Fibers ◽  
Biological Phenomenon ◽  
The Continuum

Abstract To elucidate the orientation of stress fibers in a cultured endothelial cell under cyclic stretch, we hypothesized that a stress fiber aligns so as to minimize the summation of its length change under cyclic stretch, and that there is a limit in the sensitivity of cellular response to the mechanical stimulus. Results from numerical simulations based on the continuum mechanics describe the experimental observations under uniaxial stretch well. They give us an insight to the biological phenomenon of the orientation in stress fibers under biaxial stretch from the viewpoint of mechanical engineering.

scholarly journals Activation of Na+-H+Exchange Is Necessary for RhoA-induced Stress Fiber Formation

1996 ◽  
Vol 271 (37) ◽  
pp. 22281-22284 ◽  
Author(s):  
Zinaida S. Vexler ◽  
Marc Symons ◽  
Diane L. Barber
Keyword(s):  
Stress Fiber ◽  
Fiber Formation ◽  
Stress Fiber Formation ◽  
Induced Stress
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