Mesenchymal Stem Cell Morphology in a Fibrous Microenvironment With Length Scales Matching the Native Meniscus

2012 ◽  
Author(s):  
Amy M. Silverstein ◽  
Robert L. Mauck
Keyword(s):  
Extracellular Matrix ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Morphology ◽  
Type I ◽  
Type Ii ◽  
Joint Stability ◽  
Joint Loading ◽  
Length Scales ◽  
Meniscus Healing

The meniscus is a crescent shaped fibrocartilage in the knee comprised of a circumferentially aligned collagenous extracellular matrix (ECM) that functions to transmit load and enhance joint stability. The meniscus ECM varies as a function of region, where the inner meniscus is comprised of 70% collagen (60% type II vs. 40% type I), while the outer meniscus contains 80% collagen (99% type I) [1]. Similarly, cells within the inner meniscus are rounder and more ‘chondrocyte-like’, while those in the outer meniscus are elongated and ‘fibroblast-like’ [2]. Injury of the meniscus alters joint loading and hastens the onset of osteoarthritis. As meniscus healing is limited, there exists a need for functional meniscus replacements.

scholarly journals Mesenchymal Stem Cell Seeding of Porcine Small Intestinal Submucosal Extracellular Matrix for Cardiovascular Applications

PLoS ONE ◽  
2016 ◽  
Vol 11 (4) ◽  
pp. e0153412 ◽  
Author(s):  
Chia Wei Chang ◽  
Tye Petrie ◽  
Alycia Clark ◽  
Xin Lin ◽  
Claus S. Sondergaard ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Seeding ◽  
Small Intestinal

Muscle Biopsy Samples for Histochemical Processing: Alterations Induced by Storage

1988 ◽  
Vol 25 (1) ◽  
pp. 77-82 ◽  
Author(s):  
K. G. Braund ◽  
K. A. Amling
Keyword(s):  
Skeletal Muscle ◽  
Cell Morphology ◽  
Type I ◽  
Type Ii ◽  
Tissue Samples ◽  
Frozen Tissue ◽  
Fresh Frozen ◽  
Healthy Dogs ◽  
Morphology And Morphometry ◽  
Type Ii Fibers

Skeletal muscle samples from two healthy dogs were stored in ice at 0 C for up to 30 hours to examine the influence of time on cell morphology and morphometry. Cytochemical and histochemical properties of muscle to 18 hours were not markedly different from fresh frozen tissue. Samples stored to 30 hours were still satisfactory, despite a decline and unevenness in depth of staining. Morphometry from samples stored at 0 C for 6 hours or longer is not recommended, due to the statistically significant increase in diameter (from 21 to 25%) of type I and type II fibers.

scholarly journals Extracellular matrix derived peptides and mesenchymal stem cell motility

2021 ◽  
Author(s):  
◽  
Sandi Grainne Dempsey
Keyword(s):  
Extracellular Matrix ◽  
Wound Healing ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Motility ◽  
Cell Attachment ◽  
Mass Spectrometry Analysis ◽  
And Migration ◽  
Proliferation And Migration

<p>Biomaterials derived from decellularised extracellular matrices have shown promise as tools in tissue regeneration and wound healing. Such materials display biocompatibility as well as inherent bioactivity, promoting constructive remodelling in healing tissues. In this study, the bioactivity of ovine forestomach matrix (a decellularised extracellular matrix biomaterial) is assessed based on its ability to affect the proliferation and migration of wound healing cells.  This material supported cell attachment and proliferation, but did not allow cell infiltration in vitro. Enzymatic digestion of the material rendered soluble components that were able to induce proliferation and migration of some cell types. Cell-mediated processing of the material generated a protein or proteins with chemotactic activity for mesenchymal stem cells in vitro. Mass spectrometry analysis indicated the bioactive component consisted of the proteoglycan decorin, or fragments thereof. Decorin has not previously been shown to induce mesenchymal stem cell motility, and these findings may add to what is known about decorin and its role in constructive remodelling. Furthermore, this cell-mediated approach for ECM breakdown could lead to the discovery of other bioactive peptides involved in ECM remodelling and wound healing.</p>

scholarly journals Lineage-specific exosomes could override extracellular matrix mediated human mesenchymal stem cell differentiation

Biomaterials ◽  
2018 ◽  
Vol 182 ◽  
pp. 312-322 ◽  
Author(s):  
Karthikeyan Narayanan ◽  
Sundramurthy Kumar ◽  
Parasuraman Padmanabhan ◽  
Balazs Gulyas ◽  
Andrew C.A. Wan ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Mesenchymal Stem Cell ◽  
Stem Cell Differentiation ◽  
Human Mesenchymal Stem Cell ◽  
Mesenchymal Stem Cell Differentiation

Effect of tunicamycin on maturation of fetal mouse lung

1993 ◽  
Vol 265 (3) ◽  
pp. L250-L259
Author(s):  
E. H. Webster ◽  
S. R. Hilfer ◽  
R. L. Searls ◽  
J. Kornilow
Keyword(s):  
Extracellular Matrix ◽  
Fetal Lung ◽  
Mouse Lung ◽  
Type I ◽  
Type Ii ◽  
Fetal Mouse ◽  
Type Ii Pneumocytes ◽  
Separate Control ◽  
Normal Controls

The mesodermal capsule of the fetal lung plays a role in differentiation of the respiratory region. It has been proposed for other epithelial organs that the mesodermal capsule influences development by modifying the basal lamina or the extended extracellular matrix. The effect could be on deposition or turnover of collagens, proteoglycans, and/or glycoproteins. This study tests the role of glycoproteins in differentiation of respiratory endings by inhibiting their synthesis with the antibiotic tunicamycin (TM). Lungs at 16 and 18 days gestation and 3 days after birth were cultured with TM and examined for morphological and biochemical differences from normal controls. With TM, alveolar regions did not expand properly and formed fewer type I pneumocytes, although type II pneumocytes were unaffected. The epithelium of untreated respiratory regions showed greater incorporation of radioactive mannose than the airways region or mesenchyme. This incorporation was diminished in TM, but the pattern persisted. Comparison with the results obtained with beta-xyloside suggested that differentiation of type I and type II pneumocytes is under separate control.

Multiscale grooved titanium processed with femtosecond laser influences mesenchymal stem cell morphology, adhesion, and matrix organization

2012 ◽  
Vol 100A (11) ◽  
pp. 3108-3116 ◽  
Author(s):  
Virginie Dumas ◽  
Aline Rattner ◽  
Laurence Vico ◽  
Eric Audouard ◽  
Jean Claude Dumas ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Femtosecond Laser ◽  
Cell Morphology ◽  
Matrix Organization
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