How Do Cells Sense Substrate Stiffness? Effects of Substrate Elasticity and Thickness on the Behavior of Rat Aortic Smooth Muscle Cells

2011 ◽  
Author(s):  
Takeo Matsumoto ◽  
Norihiro Matsui ◽  
Mai Ishiguro ◽  
Kazuaki Nagayama
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Cell Biology ◽  
Muscle Cells ◽  
Substrate Stiffness ◽  
Elastic Substrate ◽  
Aortic Smooth Muscle ◽  
Substrate Elasticity

There has been growing evidence that elasticity of substrate has significant effects on cells on it. For example, smooth muscle cells (SMCs) on elastic substrate increase their adhesion area as the substrate gets stiffer [1]. SMCs on substrate with elasticity gradient move to stiffer region [2]. Even differentiation of mesenchymal stem cells depends on substrate elasticity [3]. Thus, it is crucial to investigate how cells sense substrate elasticity to understand mechanical aspects of cell biology.

scholarly journals miR-30e targets IGF2-regulated osteogenesis in bone marrow-derived mesenchymal stem cells, aortic smooth muscle cells, and ApoE−/− mice

2015 ◽  
Vol 106 (1) ◽  
pp. 131-142 ◽  
Author(s):  
Wen Ding ◽  
Jihe Li ◽  
Jayanti Singh ◽  
Razan Alif ◽  
Roberto I. Vazquez-Padron ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle

scholarly journals Mesenchymal Stem Cells Attenuate Lipopolysaccharide-Induced Inflammatory Response in Human Uterine Smooth Muscle Cells

2020 ◽  
Vol 10 (03) ◽  
pp. e335-e341
Author(s):  
Arunmani Mani ◽  
John W. Hotra ◽  
Sean C. Blackwell ◽  
Laura Goetzl ◽  
Jerrie S. Refuerzo
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Smooth Muscle ◽  
Preterm Birth ◽  
Inflammatory Response ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Uterine Smooth Muscle ◽  
Cell Based Therapy ◽  
Increased Risk

Abstract Objective The aim of this study was to determine if mesenchymal stem cells (MSCs) would suppress the inflammatory response in human uterine cells in an in vitro lipopolysaccharide (LPS)-based preterm birth (PTB) model. Study Design Cocultures of human uterine smooth muscle cells (HUtSMCs) and MSCs were exposed to 5 μg/mL LPS for 4 hours and further challenged with 1 μg/mL LPS for a subsequent 24 hours. Key elements of the parturition cascade regulated by toll-like receptors (TLRs) through activation of mitogen-activated protein kinases (MAPKs) were quantified in culture supernatant as biomarkers of MSC modulation. Results Coculture with MSCs significantly attenuated TLR-4, p-JNK, and p- extracellular signal-regulated kinase 1/2 (ERK1/2) protein levels compared with HUtSMCs monoculture (p = 0.05). In addition, coculture was associated with significant inhibition of proinflammatory cytokines interleukin (IL)-6 and IL-8 (p = 0.0001) and increased production of anti-inflammatory cytokines IL-10 and transforming growth factor (TGF)-β1 (p = 0.0001). Conclusion MSCs appear to play a role in significantly attenuating LPS-mediated inflammation via alteration of down-stream MAPKs. MSCs may represent a novel, cell-based therapy in women with increased risk of inflammatory-mediated preterm birth.

scholarly journals Phenotypic Change of Mesenchymal Stem Cells Into Smooth Muscle Cells Regulated By Dynamic Cell-Surface Interactions On Patterned Arrays of Ultrathin Graphene Oxide Substrates

2021 ◽  
Author(s):  
Rowoon Park ◽  
Jung Won Yoon ◽  
Jin-Ho Lee ◽  
Suck Won Hong ◽  
Jae Ho Kim
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Mesenchymal Stem Cells ◽  
Smooth Muscle ◽  
Graphene Oxide ◽  
Smooth Muscle Cells ◽  
Self Assembly ◽  
Muscle Cells ◽  
Phenotypic Change ◽  
Cell Functions

Abstract The topographical interface of the extracellular environment has been appreciated as a principal biophysical regulator for modulating cell functions, such as adhesion, migration, proliferation, and differentiation. Despite the existed approaches that use two-dimensional nanomaterials to provide beneficial effects, opportunities evaluating their impact on stem cells remain open to elicit unprecedented cellular responses. Herein, we report an ultrathin cell-culture platform with potential-responsive nanoscale biointerfaces for monitoring mesenchymal stem cells (MSCs). We designed an intriguing nanostructured array through self-assembly of graphene oxide sheets and subsequent lithographical patterning method to produce chemophysically defined regions. MSCs cultured on anisotropic micro/nanoscale patterned substrate were spontaneously organized in a highly ordered configuration mainly due to the cell-repellent interactions. Moreover, the spatially aligned MSCs were spontaneously differentiated into smooth muscle cells upon the specific crosstalk between cells. This work provides a robust strategy for directing stem cells and differentiation, which can be utilized as a potential cell culture platform to understand cell-substrate or cell-cell interactions, further developing tissue repair and stem cell-based therapies.

scholarly journals Phenotypic change of mesenchymal stem cells into smooth muscle cells regulated by dynamic cell-surface interactions on patterned arrays of ultrathin graphene oxide substrates

2022 ◽  
Vol 20 (1) ◽  
Author(s):  
Rowoon Park ◽  
Jung Won Yoon ◽  
Jin-Ho Lee ◽  
Suck Won Hong ◽  
Jae Ho Kim
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Mesenchymal Stem Cells ◽  
Smooth Muscle ◽  
Graphene Oxide ◽  
Smooth Muscle Cells ◽  
Self Assembly ◽  
Muscle Cells ◽  
Phenotypic Change ◽  
Cell Functions

AbstractThe topographical interface of the extracellular environment has been appreciated as a principal biophysical regulator for modulating cell functions, such as adhesion, migration, proliferation, and differentiation. Despite the existed approaches that use two-dimensional nanomaterials to provide beneficial effects, opportunities evaluating their impact on stem cells remain open to elicit unprecedented cellular responses. Herein, we report an ultrathin cell-culture platform with potential-responsive nanoscale biointerfaces for monitoring mesenchymal stem cells (MSCs). We designed an intriguing nanostructured array through self-assembly of graphene oxide sheets and subsequent lithographical patterning method to produce chemophysically defined regions. MSCs cultured on anisotropic micro/nanoscale patterned substrate were spontaneously organized in a highly ordered configuration mainly due to the cell-repellent interactions. Moreover, the spatially aligned MSCs were spontaneously differentiated into smooth muscle cells upon the specific crosstalk between cells. This work provides a robust strategy for directing stem cells and differentiation, which can be utilized as a potential cell culture platform to understand cell–substrate or cell–cell interactions, further developing tissue repair and stem cell-based therapies. Graphical Abstract

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