scholarly journals Application of the Theory of Interacting Continua to Blood Flow

2011 ◽  
Author(s):  
Mehrdad Massoudi ◽  
Jeongho Kim ◽  
Samuel J. Hund ◽  
James F. Antaki
Keyword(s):  
Blood Flow ◽  
Viscous Fluid ◽  
Mixture Theory ◽  
Great Promise ◽  
Micro Scale ◽  
Small Glass ◽  
Glass Tubes ◽  
Nonlinear Fluid ◽  
Fahraeus Effect

Micro-scale investigations of the flow and deformation of blood and its formed elements have been studied for many years. Early in vitro investigations in the rotational viscometers or small glass tubes revealed important rheological properties such as the reduced blood apparent viscosity, Fahraeus effect and Fahraeus-Lindqvist effect [1], exhibiting the nonhomogeneous property of blood in microcirculation. We have applied Mixture Theory, also known as Theory of Interacting Continua, to study and model this property of blood [2, 3]. This approach holds great promise for predicting the trafficking of RBCs in micro-scale flows (such as the depletion layer near the wall), andother unique hemorheological phenomena relevant to blood trauma. The blood is assumed to be composed of an RBC component modeled as a nonlinear fluid, suspended in plasma, modeled as a linearly viscous fluid.

Determination of microvascular flow pattern formation in vivo

2000 ◽  
Vol 278 (4) ◽  
pp. H1142-H1152 ◽  
Author(s):  
Kurt Osterloh ◽  
Peter Gaehtgens ◽  
Axel R. Pries
Keyword(s):  
Blood Flow ◽  
Shear Rate ◽  
Power Spectra ◽  
Flow Patterns ◽  
Microvascular Blood Flow ◽  
Pattern Size ◽  
Branching Points ◽  
Glass Tubes

Blood flow in microvessels differs significantly from that of red blood cells (RBC) flowing through long, straight glass tubes in vitro. The in vivo situation is characterized by the presence of plasma favoring aggregation, by the irregular geometry of vessel segments, and by frequent branching points. Here, a method is presented to characterize flow patterns in microvascular blood flow during intravital microscopy based on Fourier analysis of recorded light intensity patterns. The interpretation of the resulting power spectra in terms of pattern size distribution was validated by model experiments employing artificial textures and by reverse transformation of idealized spectra. The determined size of RBC flow patterns in microvessels ranged from ∼8 μm in capillaries to ∼14 μm in vessels of >30 μm. With increasing shear rate above ∼100 s−1 pattern size increased, possibly reflecting formation of short-lived flow clusters. Below ∼100 s−1 an increase of pattern size with decreasing shear rate was found in experiments using local occlusion and treatment with high-molecular-weight dextran, suggesting the formation of aggregates. The dynamic process of generation and destruction of RBC flow patterns could well contribute to flow resistance in vivo in peripheral vascular beds.

A Modified Technique for Preliminary Screening of Compounds for Anthelmintic Activity against Eggs and Larvae of Bursate Nematodes of Sheep

1964 ◽  
Vol 38 (1-2) ◽  
pp. 47-56 ◽  
Author(s):  
I. W. Parnell
Keyword(s):  
Screening Method ◽  
Anthelmintic Activity ◽  
Modified Technique ◽  
Free Living ◽  
Preliminary Screening ◽  
Small Glass ◽  
Glass Tubes ◽  
Eggs And Larvae ◽  
Made In

A technique is described for measuring the larvicidal activity of compounds against the free-living stages of bursate nematodes of sheep. Cultures were made in small glass tubes in 2 oz. “pomade” jars, and the larvicidal effects of the compounds were measured by comparing the number of infective larvae which were recovered from treated cultures with the numbers from the control cultures.This technique provides another in vitro screening method for compounds which may be effective as anthelmintics against bursate nematodes.

Effect of caffeine on theophyliine on cerebral blood flow response to brain activation: In vitro and in vivo studies

2005 ◽  
Vol 25 (1_suppl) ◽  
pp. S198-S198
Author(s):  
Joseph R Meno ◽  
Thien-son K Nguyen ◽  
Elise M Jensen ◽  
G Alexander West ◽  
Leonid Groysman ◽  
...  
Keyword(s):  
Blood Flow ◽  
Cerebral Blood Flow ◽  
Brain Activation ◽  
In Vivo Studies ◽  
Blood Flow Response ◽  
Flow Response

The Mesothelial Cell as a Non-Thrombogenic Surface

1984 ◽  
Vol 52 (02) ◽  
pp. 102-104 ◽  
Author(s):  
L J Nicholson ◽  
J M F Clarke ◽  
R M Pittilo ◽  
S J Machin ◽  
N Woolf
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Blood Flow ◽  
Cell Surface ◽  
Mesothelial Cell ◽  
Mesothelial Cells ◽  
Plastic Film ◽  
In Vitro System ◽  
Scanning Electron

SummaryA technique for harvesting mesothelial cells is described. This entails collagenase digestion of omentum after which the cells can be cultured. The technique has been developed using the rat, but has also been successfully applied to human tissue. Cultured rat mesothelial cells obtained in this way have been examined by scanning electron microscopy. Rat mesothelial cells grown on plastic film have been exposed to blood in an in vitro system using a Baumgartner chamber and have been demonstrated to support blood flow. No adhering platelets were observed on the mesothelial cell surface. Fibroblasts similarily exposed to blood as a control were washed off the plastic.

scholarly journals Impairing flow-mediated endothelial remodeling reduces extravasation of tumor cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Gautier Follain ◽  
Naël Osmani ◽  
Valentin Gensbittel ◽  
Nandini Asokan ◽  
Annabel Larnicol ◽  
...  
Keyword(s):  
Blood Flow ◽  
Tumor Cells ◽  
Molecular Mechanisms ◽  
Metastatic Progression ◽  
Metastatic Dissemination ◽  
Secondary Tumors ◽  
Flow Profiles ◽  
Endothelial Response ◽  
Life Threatening

AbstractTumor progression and metastatic dissemination are driven by cell-intrinsic and biomechanical cues that favor the growth of life-threatening secondary tumors. We recently identified pro-metastatic vascular regions with blood flow profiles that are permissive for the arrest of circulating tumor cells. We have further established that such flow profiles also control endothelial remodeling, which favors extravasation of arrested CTCs. Yet, how shear forces control endothelial remodeling is unknown. In the present work, we aimed at dissecting the cellular and molecular mechanisms driving blood flow-dependent endothelial remodeling. Transcriptomic analysis of endothelial cells revealed that blood flow enhanced VEGFR signaling, among others. Using a combination of in vitro microfluidics and intravital imaging in zebrafish embryos, we now demonstrate that the early flow-driven endothelial response can be prevented upon specific inhibition of VEGFR tyrosine kinase and subsequent signaling. Inhibitory targeting of VEGFRs reduced endothelial remodeling and subsequent metastatic extravasation. These results confirm the importance of VEGFR-dependent endothelial remodeling as a driving force of CTC extravasation and metastatic dissemination. Furthermore, the present work suggests that therapies targeting endothelial remodeling might be a relevant clinical strategy in order to impede metastatic progression.

scholarly journals High-throughput screening and rational design of biofunctionalized surfaces with optimized biocompatibility and antimicrobial activity

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Zhou Fang ◽  
Junjian Chen ◽  
Ye Zhu ◽  
Guansong Hu ◽  
Haoqian Xin ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
High Throughput ◽  
High Throughput Screening ◽  
Rational Design ◽  
Click Reaction ◽  
Reaction Times ◽  
Great Promise ◽  
Biomaterial Surfaces

AbstractPeptides are widely used for surface modification to develop improved implants, such as cell adhesion RGD peptide and antimicrobial peptide (AMP). However, it is a daunting challenge to identify an optimized condition with the two peptides showing their intended activities and the parameters for reaching such a condition. Herein, we develop a high-throughput strategy, preparing titanium (Ti) surfaces with a gradient in peptide density by click reaction as a platform, to screen the positions with desired functions. Such positions are corresponding to optimized molecular parameters (peptide densities/ratios) and associated preparation parameters (reaction times/reactant concentrations). These parameters are then extracted to prepare nongradient mono- and dual-peptide functionalized Ti surfaces with desired biocompatibility or/and antimicrobial activity in vitro and in vivo. We also demonstrate this strategy could be extended to other materials. Here, we show that the high-throughput versatile strategy holds great promise for rational design and preparation of functional biomaterial surfaces.

scholarly journals Endothelial deletion of PKCδ prevents VEGF inhibition and restores blood flow reperfusion in diabetic ischemic limb

2021 ◽  
Vol 18 (2) ◽  
pp. 147916412199903
Author(s):  
Laura Croteau ◽  
Clément Mercier ◽  
Étienne Fafard-Couture ◽  
Alexandre Nadeau ◽  
Stéphanie Robillard ◽  
...  
Keyword(s):  
Blood Flow ◽  
Phosphatase Activity ◽  
Capillary Density ◽  
Artery Ligation ◽  
Vegf Signaling ◽  
Post Surgery ◽  
Src Homology ◽  
Artery Disease ◽  
Ischemic Muscle

Aims: Peripheral artery disease is a complication of diabetes leading to critical hindlimb ischemia. Diabetes-induced inhibition of VEGF actions is associated with the activation of protein kinase Cδ (PKCδ). We aim to specifically investigate the role of PKCδ in endothelial cell (EC) function and VEGF signaling. Methods: Nondiabetic and diabetic mice, with ( ec-Prkcd−/−) or without ( ec-Prkcdf/f) endothelial deletion of PKCδ, underwent femoral artery ligation. Blood flow reperfusion was assessed up to 4 weeks post-surgery. Capillary density, EC apoptosis and VEGF signaling were evaluated in the ischemic muscle. Src homology region 2 domain-containing phosphatase-1 (SHP-1) phosphatase activity was assessed in vitro using primary ECs. Results: Ischemic muscle of diabetic ec-Prkcdf/f mice exhibited reduced blood flow reperfusion and capillary density while apoptosis increased as compared to nondiabetic ec-Prkcdf/f mice. In contrast, blood flow reperfusion and capillary density were significantly improved in diabetic ec-Prkcd−/− mice. VEGF signaling pathway was restored in diabetic ec-Prkcd−/− mice. The deletion of PKCδ in ECs prevented diabetes-induced VEGF unresponsiveness through a reduction of SHP-1 phosphatase activity. Conclusions: Our data provide new highlights in mechanisms by which PKCδ activation in EC contributed to poor collateral vessel formation, thus, offering novel therapeutic targets to improve angiogenesis in the diabetic limb.

scholarly journals Obtaining Expressions for Time-Dependent Functions That Describe the Unsteady Properties of Swirling Jets of Viscous Fluid

Mathematics ◽  
2021 ◽  
Vol 9 (16) ◽  
pp. 1860
Author(s):  
Eugene Talygin ◽  
Alexander Gorodkov
Keyword(s):  
Blood Flow ◽  
Viscous Fluid ◽  
Swirling Flow ◽  
Fluid Flows ◽  
Theoretical Method ◽  
Flow Channel ◽  
Navier Stokes ◽  
Swirling Jets ◽  
Continuity Equations ◽  
Dynamic Velocity

Previously, it has been shown that the dynamic geometric configuration of the flow channel of the left heart and aorta corresponds to the direction of the streamlines of swirling flow, which can be described using the exact solution of the Navier–Stokes and continuity equations for the class of centripetal swirling viscous fluid flows. In this paper, analytical expressions were obtained. They describe the functions C0t and Г0t, included in the solutions, for the velocity components of such a flow. These expressions make it possible to relate the values of these functions to dynamic changes in the geometry of the flow channel in which the swirling flow evolves. The obtained expressions allow the reconstruction of the dynamic velocity field of an unsteady potential swirling flow in a flow channel of arbitrary geometry. The proposed approach can be used as a theoretical method for correct numerical modeling of the blood flow in the heart chambers and large arteries, as well as for developing a mathematical model of blood circulation, considering the swirling structure of the blood flow.

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