Fibroblast Behavior on Tunable Gels With Decreasing Elasticity

ASME 2011 Summer Bioengineering Conference, Parts A and B ◽

10.1115/sbc2011-53251 ◽

2011 ◽

Author(s):

Michelle L. Previtera ◽

Kevin Trout ◽

Uday Chippada ◽

Rene Schloss ◽

Noshir A. Langrana

Keyword(s):

Extracellular Matrix ◽

Elastic Properties ◽

Polyacrylamide Gel ◽

External Factors ◽

Culture Period ◽

Cell Behavior ◽

Mechanical Stiffness ◽

Dynamic Changes ◽

Physical Cues

Cells sense and react to various extracellular matrix (ECM) cues including chemical and physical cues. Previous studies in our laboratory and others have used static substrates, where the elastic properties remain unchanged throughout the culture period, to examine the effects of mechanical stiffness on neuron and fibroblast behavior [1–4]. However, in vivo, the ECM is dynamic and alters due to pathological, developmental, and external factors [5]. To study the effects of dynamic ECM changes on cell behavior, we developed a DNA-crosslinked, polyacrylamide gel (DNA gel) that allows us to study how dynamic changes in ECM stiffness affect cell behavior [6, 7].

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Effects of soluble factors and extracellular matrix components on vascular cell behavior in vitro and in vivo: Models of de-endothelialization and repair

Journal of Cellular Biochemistry ◽

10.1002/jcb.240450202 ◽

1991 ◽

Vol 45 (2) ◽

pp. 123-130 ◽

Cited By ~ 74

Author(s):

Joseph A. Madri ◽

Martin Marx ◽

June R. Merwin ◽

Craig Basson ◽

Christian Prinz ◽

...

Keyword(s):

Extracellular Matrix ◽

Cell Behavior ◽

Soluble Factors ◽

Vascular Cell ◽

In Vivo Models ◽

Extracellular Matrix Components ◽

Matrix Components

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Extracellular matrix: the gatekeeper of tumor angiogenesis

Biochemical Society Transactions ◽

10.1042/bst20190653 ◽

2019 ◽

Vol 47 (5) ◽

pp. 1543-1555 ◽

Cited By ~ 10

Author(s):

Maurizio Mongiat ◽

Simone Buraschi ◽

Eva Andreuzzi ◽

Thomas Neill ◽

Renato V. Iozzo

Keyword(s):

Extracellular Matrix ◽

Tumor Angiogenesis ◽

Signaling Cascades ◽

Cancer Growth ◽

Cell Behavior ◽

Metastatic Progression ◽

Surface Receptors ◽

The Matrix ◽

Multiple Signaling

Abstract The extracellular matrix is a network of secreted macromolecules that provides a harmonious meshwork for the growth and homeostatic development of organisms. It conveys multiple signaling cascades affecting specific surface receptors that impact cell behavior. During cancer growth, this bioactive meshwork is remodeled and enriched in newly formed blood vessels, which provide nutrients and oxygen to the growing tumor cells. Remodeling of the tumor microenvironment leads to the formation of bioactive fragments that may have a distinct function from their parent molecules, and the balance among these factors directly influence cell viability and metastatic progression. Indeed, the matrix acts as a gatekeeper by regulating the access of cancer cells to nutrients. Here, we will critically evaluate the role of selected matrix constituents in regulating tumor angiogenesis and provide up-to-date information concerning their primary mechanisms of action.

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Desmopressin (DDAVP) Enhances Platelet Adhesion to the Extracellular Matrix of Cultured Human Endothelial Cells through Increased Expression of Tissue Factor

Thrombosis and Haemostasis ◽

10.1055/s-0038-1656088 ◽

1997 ◽

Vol 77 (05) ◽

pp. 0975-0980 ◽

Cited By ~ 23

Author(s):

Angel Gálvez ◽

Goretti Gómez-Ortiz ◽

Maribel Díaz-Ricart ◽

Ginés Escolar ◽

Rogelio González-Sarmiento ◽

...

Keyword(s):

Extracellular Matrix ◽

Endothelial Cells ◽

Tissue Factor ◽

Platelet Adhesion ◽

Umbilical Vein ◽

Procoagulant Activity ◽

Experimental Conditions ◽

Chromogenic Assay

SummaryThe effect of desmopressin (DDAVP) on thrombogenicity, expression of tissue factor and procoagulant activity (PCA) of extracellular matrix (ECM) generated by human umbilical vein endothelial cells cultures (HUVEC), was studied under different experimental conditions. HUVEC were incubated with DDAVP (1, 5 and 30 ng/ml) and then detached from their ECM. The reactivity towards platelets of this ECM was tested in a perfusion system. Coverslips covered with DD A VP-treated ECMs were inserted in a parallel-plate chamber and exposed to normal blood anticoagulated with low molecular weight heparin (Fragmin®, 20 U/ml). Perfusions were run for 5 min at a shear rate of 800 s1. Deposition of platelets on ECMs was significantly increased with respect to control ECMs when DDAVP was used at 5 and 30 ng/ml (p <0.05 and p <0.01 respectively). The increase in platelet deposition was prevented by incubation of ECMs with an antibody against human tissue factor prior to perfusion. Immunofluorescence studies positively detected tissue factor antigen on DDAVP derived ECMs. A chromogenic assay performed under standardized conditions revealed a statistically significant increase in the procoagulant activity of the ECMs produced by ECs incubated with 30 ng/ml DDAVP (p <0.01 vs. control samples). Northern blot analysis revealed increased levels of tissue factor mRNA in extracts from ECs exposed to DDAVP. Our data indicate that DDAVP in vitro enhances platelet adhesion to the ECMs through increased expression of tissue factor. A similar increase in the expression of tissue factor might contribute to the in vivo hemostatic effect of DDAVP.

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