Agarose Concentration and TGF-B3 Supplementation Influence Matrix Deposition in Engineered Cartilage Constructs

2011 ◽  
Author(s):  
Linda M. Kock ◽  
Corrinus C. van Donkelaar ◽  
Keita Ito
Keyword(s):  
Articular Cartilage ◽  
Cartilage Repair ◽  
Structural Organization ◽  
Influence Matrix ◽  
Matrix Deposition ◽  
Engineer Cartilage ◽  
High Prevalence ◽  
Proteoglycan Content ◽  
Tissue Engineer Cartilage ◽  
Engineered Cartilage

High prevalence of osteoarthritis and poor intrinsic healing capacity of articular cartilage create a demand for cell-based strategies for cartilage repair. It is possible to tissue engineer cartilage with almost native proteoglycan content, but collagen reaches only 15% to 35% of the native content. Also its natural structural organization is not reproduced. These drawbacks contribute to its insufficient load-bearing properties.

Mechanical Stimulation Enhances Collagen Synthesis in Periosteum-Derived Cartilage

2009 ◽  
Author(s):  
Agnese Ravetto ◽  
Linda M. Kock ◽  
Corrinus C. van Donkelaar ◽  
Keita Ito
Keyword(s):  
Mechanical Properties ◽  
Articular Cartilage ◽  
Mechanical Loading ◽  
Cartilage Repair ◽  
Structural Organization ◽  
Collagen Synthesis ◽  
Engineer Cartilage ◽  
High Prevalence ◽  
Proteoglycan Content ◽  
Tissue Engineer Cartilage

High prevalence of osteoarthritis and poor intrinsic healing capacity of articular cartilage create a demand for cell-based strategies for cartilage repair. It is possible to tissue engineer cartilage with almost native proteoglycan content, but collagen reaches only 15% to 35% of the native content. Also its natural arcade-like structural organization is not reproduced. These drawbacks contribute to its insufficient load-bearing properties. It is generally believed that the application of mechanical loading during culturing will improve the mechanical properties. However, a suitable mechanical loading regime has not yet been established.

Influence of Interleukin Treatment on Engineered and Native Articular Cartilage

2007 ◽  
Author(s):  
Eric G. Lima ◽  
Liming Bian ◽  
Francis B. Gonzales ◽  
Gerard A. Ateshian ◽  
Clark T. Hung
Keyword(s):  
Articular Cartilage ◽  
Cartilage Repair ◽  
Biochemical Properties ◽  
Inflammatory Factors ◽  
Stages Of Development ◽  
Repair Tissue ◽  
Native Cartilage ◽  
Diarthrodial Joint ◽  
Inflammatory Molecules ◽  
Engineered Cartilage

Injury to the diarthrodial joint is often associated with elevated levels of cytokines and other inflammatory molecules. While the influence of interleukin on articular cartilage has been well-studied, its effects on engineered cartilage are not. The presence of inflammatory factors in the injured joint would be expected to affect the performance of implanted engineered cartilage repair tissue [1] and this effect may be especially pronounced in underdeveloped tissues [2]. The current study addresses this issue by examining the influence of interleukin (IL-1α and IL-1β) on engineered cartilage mechanical and biochemical properties at sequential stages of development. Furthermore, dexamethasone, an anti-inflammatory steroid that has been shown in some cases to suppress interleukin-induced degradation of native cartilage [3], was examined in the context of engineered constructs.

scholarly journals Articular cartilage repair using a tissue-engineered cartilage-like implant: an animal study

2001 ◽  
Vol 9 ◽  
pp. S6-S15 ◽  
Author(s):  
P. Mainil-Varlet ◽  
F. Rieser ◽  
S. Grogan ◽  
W. Mueller ◽  
C. Saager ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Animal Study ◽  
Cartilage Repair ◽  
Articular Cartilage Repair ◽  
Engineered Cartilage

scholarly journals Heterogeneity of mesenchymal stem cells in cartilage regeneration: from characterization to application

2021 ◽  
Vol 6 (1) ◽  
Author(s):  
Kangkang Zha ◽  
Xu Li ◽  
Zhen Yang ◽  
Guangzhao Tian ◽  
Zhiqiang Sun ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Articular Cartilage ◽  
Cartilage Repair ◽  
Cartilage Damage ◽  
Cartilage Regeneration ◽  
Great Promise ◽  
Traditional Treatment ◽  
Different Types ◽  
Selection Of

AbstractArticular cartilage is susceptible to damage but hard to self-repair due to its avascular nature. Traditional treatment methods are not able to produce satisfactory effects. Mesenchymal stem cells (MSCs) have shown great promise in cartilage repair. However, the therapeutic effect of MSCs is often unstable partly due to their heterogeneity. Understanding the heterogeneity of MSCs and the potential of different types of MSCs for cartilage regeneration will facilitate the selection of superior MSCs for treating cartilage damage. This review provides an overview of the heterogeneity of MSCs at the donor, tissue source and cell immunophenotype levels, including their cytological properties, such as their ability for proliferation, chondrogenic differentiation and immunoregulation, as well as their current applications in cartilage regeneration. This information will improve the precision of MSC-based therapeutic strategies, thus maximizing the efficiency of articular cartilage repair.

Finite Element Modeling of Osmotic Swelling in Tissue-Engineered Cartilage

2008 ◽  
Author(s):  
Liming Bian ◽  
Terri Ann N. Kelly ◽  
Eric G. Lima ◽  
Gerard A. Ateshian ◽  
Clark T. Hung
Keyword(s):  
Articular Cartilage ◽  
Potential Role ◽  
Swelling Pressure ◽  
Type Ii Collagen ◽  
Type Ii ◽  
Osmotic Swelling ◽  
Fixed Charges ◽  
Central Regions ◽  
Significant Research ◽  
Engineered Cartilage

Proteoglycans and Type II collagen represent the two major biochemical constituents of articular cartilage. Collagen fibrils in cartilage resist the swelling pressure that arises from the fixed charges of the glycosaminoglycans (GAGs), and together they give rise to the tissue’s unique load bearing properties. As articular cartilage exhibits a poor intrinsic healing capacity, there is significant research in the development of cell-based therapies for cartilage repair. In some of our tissue engineering studies, we have observed a phenomenon where chondrocyte-seeded hydrogel constructs display cracking in their central regions after significant GAG content has been elaborated in culture. A theoretical analysis was performed to gain greater insights into the potential role that the spatial distribution of proteoglycan and collagen may play in this observed response.

scholarly journals Loss of proteoglycan content primes articular cartilage for mechanically induced damage

2018 ◽  
Vol 26 ◽  
pp. S371 ◽  
Author(s):  
M.E. Cooke ◽  
B.M. Lawless ◽  
S.W. Jones ◽  
L.M. Grover
Keyword(s):  
Articular Cartilage ◽  
Proteoglycan Content
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